Study of the obp5 gene in Apis mellifera ligustica and Apis cerana cerana

Zhao, Huiting; Zeng, Xiaoyue; Liang, Qifu; Zhang, X F; Huang, Wenyan; Chen, H S; Luo, Yuexiong

doi:10.4238/2015.june.12.1

Cited by 14 publications

(11 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A. cerana cerana is the major honeybee species in China and plays an important role in ecological balance and the agricultural industry. A. cerana cerana has the advantages of a long period of collecting honey, low food cost and good resistance to disease [24,25]. However, in recent years, the survival of A. cerana cerana has been seriously threatened due to various environmental stresses including the indiscriminate use of pesticides, infectious diseases, and global warming [26].…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Wang

Cui

et al. 2018

Bioscience Reports

View full text Add to dashboard Cite

Mitogen-activated protein kinase kinases (MKKs) are important components of the MAPK signaling pathways, which play a key role in responding to stress and inflammatory stimuli. Here, a new MKK gene, AccMKK6, was identified and functionally analyzed in Apis cerana cerana. Real-time quantitative PCR (qPCR) and Western blot analysis demonstrated that the AccMKK6 expression level was up-regulated by several environmental stresses. Moreover, the knockdown of AccMKK6 by RNA interference technology altered the expression levels of some antioxidant genes. In addition, the knockdown of AccMKK6 resulted in increased malonyldialdehyde (MDA) concentration and decreased antioxidant-related enzymes activity in honeybees. To explore the MAPK signaling pathways involved in AccMKK6, we identified the transcription factor kayak in A. cerana cerana. We analyzed the interactions of AccMKK6, Accp38b, and Acckayak using the yeast two-hybrid system. AccMKK6 and Acckayak showed similar expression profiles after several stress treatments. In addition, the expression level of Acckayak was significantly increased when AccMKK6 was silenced. Therefore, we speculate that AccMKK6 may be involved in the MAPK cascades, which play a crucial role in counteracting oxidative stress caused by external stimuli.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Wang

Cui

et al. 2018

Bioscience Reports

View full text Add to dashboard Cite

show abstract

“…In particular, the affinity and selectivity of the OBP2 protein in plant odors has been demonstrated, showing its physiological role in the recognition of forage species, especially in those that present compounds such as 1-8 cineole in their aroma [58]. Additionally, Zhao et al [59] reported the crucial role of the OBP5 protein in the larval care processes within the hive. This protein has an affinity for volatile compounds released by larvae when they are affected by different pathogens, suggesting that the recognition of these volatile compounds by nurse bees could aid in recognizing diseased larvae and caring for health larvae, being key in the sanitary maintenance of the brood within the hive.…”

Section: Discussionmentioning

confidence: 98%

Variant A of the Deformed Wings Virus Alters the Olfactory Sensitivity and the Expression of Odorant Binding Proteins on Antennas of Apis mellifera

Silva

Ceballos

Arismendi

et al. 2021

Insects

View full text Add to dashboard Cite

Insects have a highly sensitive sense of smell, allowing them to perform complex behaviors, such as foraging and peer recognition. Their sense of smell is based on the recognition of ligands and is mainly coordinated by odorant-binding proteins (OBPs). In Apis mellifera, behavior can be affected by different pathogens, including deformed wing virus (DWV) and its variants. In particular, it has been shown that variant A of DWV (DWV-A) is capable of altering the ultra-cellular structure associated with olfactory activity. In this study was evaluated olfactory sensitivity and the expression of OBP genes in honey bees inoculated with DWV-A. Electroantennographic analyses (EAG) were carried out to determine the olfactory sensitivity to the essential oils Eucalyptus globulus and Mentha piperita. The expression of nine antenna-specific OBP genes and DWV-A load in inoculated bees was also quantified by qPCR. We observed an inverse relationship between viral load and olfactory sensitivity and the expression of some OBP proteins. Thus, high viral loads reduced olfactory sensitivity to essential oils and the gene expression of the OBP2, OBP5, OBP11, and OBP12 proteins on the antennas of middle- and forager-age bees. These results suggest that DWV-A could have negative effects on the processes of aroma perception by worker bees, affecting their performance in tasks carried out in and outside the colony.

show abstract

“…Such hygienic behavior can accordingly enable bee colonies to resist a range of potentially devastating diseases such as European foulbrood and chalkbrood disease [8][9][10]. In this regard, Eastern honeybees (Apis cerana) are considered to be somewhat better adapted than Western honeybees (Apis mellifera), in that they carry fewer pathogens and parasites [11][12][13][14][15][16][17] and produce a more diverse array of antimicrobial peptides when attacked or irritated by pathogens, thereby minimizing damage [18]. Compared with A. mellifera, A. cerana bees are thus considered to be more resistant to pathogens and more e cient with respect to their hygienic behavior, in terms of both uncapping and removal [19,20].…”

Section: Introductionmentioning

confidence: 99%

Difference between uncapping and removal behaviors in Apis cerana from the perspective of long non-coding RNAs

Li,

Yang,

You

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Hygienic behavior has been identified as a specific type of immune response that has evolved in social insects, in which it contributes to preventing the spread of diseases throughout insect colonies. In bee colonies, such hygienic behavior entails the dual steps of uncapping and removal of dead and diseased larvae and pupae. Although in recent years, numerous studies have examined the development of hygienic behavior among bees, the mechanisms underlying the division in the performance of uncapping and removal have yet to be sufficiently elucidated. In this regard, long non-coding RNAs (lncRNAs) have been evidenced to be engaged in regulating the physiological activities of honeybees; however, whether lncRNAs are similarly involved in the uncapping and removal behaviors of these bees has not been clarified. Results In this study, the strong hygienic Apis cerana worker bees were used and the processes of uncapping and removal behaviors in three colonies were assayed with freeze-killed brood in the field. We then sequenced the antennal RNAs of honeybees to identify differentially expressed lncRNAs and performed lncRNA–mRNA association analysis to establish the differences between uncapping and removal. We detected 1323 differentially expressed lncRNAs in the antennae, and the findings of lncRNA–mRNA association analyses revealed that the target genes of differentially expressed lncRNAs between uncapping and removal worker bees were mainly annotated to response to stimulus, receptor activity, and synapse. Among the lncRNAs enriched in cellular response to stimulus, XR_001766094.2 was identified to be uniquely expressed in the uncapping workers bees. We accordingly speculate that by responding to external stimuli, XR_001766094.2 may play a key role in the distinction between uncapping and removal, thereby indicating that the division of hygienic behaviors is determined by a differential response to environmental stimuli. Conclusion We characterized differences in the uncapping and removal behaviors of worker bees from perspectives of lncRNAs. The uncapping bees may equip with a more rapid stimulatory response and more acute olfactory sensitivity, contributing to the rapid hygienic behavior in honeybee colonies. Our study thus laid the foundation for the potential lncRNA-mediated expression regulation of genes in hygienic behavior.

show abstract

Study of the obp5 gene in Apis mellifera ligustica and Apis cerana cerana

Cited by 14 publications

References 27 publications

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Variant A of the Deformed Wings Virus Alters the Olfactory Sensitivity and the Expression of Odorant Binding Proteins on Antennas of Apis mellifera

Difference between uncapping and removal behaviors in Apis cerana from the perspective of long non-coding RNAs

Contact Info

Product

Resources

About