The aim of this work was to investigate the nanomechanical, adhesion and corrosion resistance of hydroxyapatite (HAP) coatings. The electrodeposition process was used to elaborate the HAP coatings on Ti6Al4V alloy. The effect of hydrogen peroxide concentration H2O2 on the electrolyte and the heat treatment was studied. Surface morphology of HAP coatings was assessed, before and after heat treatment, by scanning electron microscopy associated with X-ray microanalysis (SEM-EDXS). Moreover, X-ray diffraction (XRD) was performed to identify the coatings’ phases and composition. Nanoindentation and scratch tests were performed for nanomechanical and adhesion behavior analysis. The corrosion resistance of the uncoated, the as-deposited, and the heat-treated coatings was investigated by electrochemical test. The obtained results revealed that, with 9% of H2O2 and after heat treatment, the HAP film exhibited a compact and homogeneous microstructure. The film also showed a crystal growth: stoichiometric hydroxyapatite (HAP) and β-tricalcium phosphate (β-TCP). After heat treatment, the nanomechanical properties (H, E) were increased from 117 ± 7 MPa and 24 ± 1 GPa to 171 ± 10 MPa and 38 ± 1.5 GPa respectively. Critical loads (LC1, LC2, and LC3) were increased from 0.78 ± 0.04, 1.6 ± 0.01, and 4 ± 0.23 N to 1.45 ± 0.08, 2.46 ± 0.14, and 4.35 ± 0.25 N (respectively). Furthermore, the adhesion strength increased from 8 to 13 MPa after heat treatment. The HAP heat-treated samples showed higher corrosion resistance (Rp = 65.85 kΩ/cm2; Icorr = 0.63 µA/cm2; Ecorr = −167 mV/ECS) compared to as-deposited and uncoated samples.