Study of the osteoprotegerin/receptor activator of nuclear factor-kB ligand system association with inflammation and atherosclerosis in systemic sclerosis

Gamal, Rania M.; Gamal, Walid M; Ghandour, Abeer M.; Abozaid, Hanan S.; Mohamed, Mai; Emad, Yasser; Abdel-Galeel, Ahmed

doi:10.1080/08820139.2017.1423499

Cited by 18 publications

(10 citation statements)

References 35 publications

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“…18,33 Along with the ongoing process of atherosclerosis and in the late stages of lesion progress, OPG may cause some kind of injury to the vessels where it is incapable to reverse the process of vascular calcification. 18,34 It should be mentioned that OPG not only modulates RANKL but also acts in some RANKL-independent manners such as chemotaxis of monocytes. 35 In our study, there were no significant differences in serum levels as well as gene expression of OPG, RANK, and RANKL among different severity groups of UA according to TIMI and GRACE scores.…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Serum and Gene Expression Levels of RANK, RANKL and Osteoprotegerin Inflammatory Pathway with Unstable Angina: A Case-control Study

Farrokhian¹,

Miraftab²,

Chenari³

et al. 2021

IJAAI

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Osteoprotegerin (OPG), receptor activator of nuclear factor-kappa B (RANK) and receptor activator of nuclear factor-kappa B ligand (RANKL), the members of the tumor necrosis factor (TNF) family, have multiple effects on bone metabolism, endocrine functions and, as an inflammatory pathway, in the immune system. This study tried to determine the association of the OPG/RANKL/RANK axis with the severity of unstable angina (UA) as an inflammatory condition. Our study involved 50 patients with UA and 50 healthy people. Serum and peripheral blood mononuclear cells were isolated from all participants. Serum levels and gene expression of OPG, RANKL, and RANK in mononuclear cells were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR), respectively. For each patient with UA, the thrombolysis in myocardial infarction (TIMI) and the global registry of acute coronary events (GRACE) scores were determined to evaluate the severity of the disease. Then we analyzed the relation of OPG, RANKL, and RANK levels with TIMI and GRACE scores in patients with UA. Discriminate analysis was used to predict the combinational models of such factors on the prediction of UA. Serum levels of OPG and RANKL (p<0.001) and gene expression of RANKL (p<0.001) were significantly more in patients than those in healthy ones. No relation was seen between the OPG/RANKL/RANK axis and the severity of UA according to TIMI and GRACE scores. Our study shows that serum level, as well as gene expression of OPG/RANKL/RANK axis neither, predicts the occurrence of UA nor shows any relationship with its severity.

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Section: Discussionmentioning

confidence: 99%

The Relationship between Serum and Gene Expression Levels of RANK, RANKL and Osteoprotegerin Inflammatory Pathway with Unstable Angina: A Case-control Study

Farrokhian¹,

Miraftab²,

Chenari³

et al. 2021

IJAAI

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“…Одним из возможных механизмов формирования АС, учитывая результаты настоящей работы, является нарушение чувствительности OPG к RANKL, второй возможной причиной является повышение концентрации sRANKL у больных с АС. Gamal RM, et al анализируя активность системы OPG/RANKL сделал вывод, что повышение OPG является компенсаторным механизмом, направленным на нейтрализацию sRANKL, а соотношение OPG/ RANKL может рассматриваться как диагностический индикатор тяжести сосудистой кальцификации и атеросклероза [12]. Подобной гипотезой можно объяснить и данные, полученные в нашем исследовании.…”

Section: Discussionunclassified

The Role of Osteoprotegerin System /Rankl/Rank in Pathogenesis of Aortic Stenosis

Иртюга¹,

Жидулева²,

Murtazalieva³

et al. 2018

Russ J Cardiol

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Цель. Оценить концентрацию остеопротегерина (OPG) и растворимого лиганда рецептора активатора фактора транскрипции каппа-В (sRANKL) в сыворотке крови у больных с различной степенью тяжести аортального стеноза (АС). Материал и методы. Обследовано 247 больных с АС различной степени тяжести: 46 пациентов с легкой степенью тяжести, 53 пациента с умеренным АС и 149 с тяжелым АС. Из них 132 (53%) пациента с бикуспидальным аортальным клапаном (БАК) и 115 (47%) с трикуспидальным аортальным клапаном (ТАК). Контрольную группу составили 58 пациентов без клапанной патологии сердца и ишемической болезни сердца. У всех пациентов определяли липидный профиль, сывороточный уровень С-реактивного белка (СРБ), OPG и sRANKL. Результаты. Во всех исследуемых группах больных с АС выявлено повышение содержания sRANKL в сыворотке крови в сравнении с контрольной группой (БАК =0,37 [0,32;0,53] пмоль/л, ТАК =0,38 [0,33;0,50] пмоль/л, контрольная группа 0,30 [0,21;0,39] пмоль/л; р<0,0001. Концентрация OPG была повышена только у больных с ТАК: 6,99 [5,19;9,90] пмоль/л; по сравнению с 5,23 [4,30;7,09] пмоль/л у пациентов с БАК (р=0,0008). Высказана гипотеза, что рост концентрации OPG является компенсаторным и возникает в ответ на повышение уровня или нарушение чувствительности к sRANKL. Заключение. Развитие АС сопряжено с нарушениями в системе OPG/RANKL, а выявление этих изменений может иметь важное диагностическое и прогностическое значение, особенно у пациентов с ТАК. Российский кардиологический журнал 2018, 2 (154): 39-43 http://dx.Ключевые слова: аортальный стеноз, кальциноз, биомаркеры, остеопротегерин, sRANKL. Russ J Cardiol 2018, 2 (154): 39-43 http://dx.

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“…[8] RANKL is mainly secreted by osteoblast and activated T cells and binds to receptor activator of nuclear factor-kB (TNF Receptor Superfamily 11A, TNFRSF11A, RANK) to induce the maturation and activation of osteoclast precursors and motivate bone resorption. [9] Decoy receptor belonging to TNF receptor superfamily called osteoprotegerin (TNFRSF11B, OPG) inhibits the binding of RANKL to RANK then suppressing the osteoclastogenesis. [10] Imbalance of the RANKL/RANK/OPG concentration ratio is involved in bone remodeling and consequently loss of bone density in a large number of immune system diseases including osteoporosis [11] or RA.…”

Section: Introductionmentioning

confidence: 99%

“…Among cytokine families, receptor activator of nuclear factor‐κB ligand (RANKL), also known as tumor necrosis factor‐related activation‐induced cytokine (TRANCE), is a homotrimeric type II membrane protein member of the TNF‐α superfamily (TNF Superfamily 11, TNFSF11) . RANKL is mainly secreted by osteoblast and activated T cells and binds to receptor activator of nuclear factor‐κB (TNF Receptor Superfamily 11A, TNFRSF11A, RANK) to induce the maturation and activation of osteoclast precursors and motivate bone resorption . Decoy receptor belonging to TNF receptor superfamily called osteoprotegerin (TNFRSF11B, OPG) inhibits the binding of RANKL to RANK then suppressing the osteoclastogenesis .…”

Section: Introductionmentioning

confidence: 99%

Carbon/Inorganic Hybrid Nanoarchitectures as Carriers for Signaling Elements in Electrochemical Immunosensors: First Biosensor for the Determination of the Inflammatory and Metastatic Processes Biomarker RANK‐ligand

et al. 2020

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This work reports the development of the first integrated electrochemical immunosensor for the determination of the ligand receptor activator nuclear factor‐κB (RANKL), a biomarker that plays an important role in the regulation of bone resorption process and, as reported recently, in oncology by modulating the immune system response facilitating the tumor growth and metastatic process during cancer development. The developed platform involves a sandwich‐type immunoassay with covalent immobilization of biotinylated capture antibodies onto streptavidin/4‐aminobenzoic acid (p‐ABA) grafted screen‐printed carbon electrodes (bCAb‐Strep/p‐ABA‐SPCE) and the use of an hybrid nanomaterial composed of gold nanoparticles (AuNPs) and multi‐walled carbon nanotubes (MWCNTs) as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the H2O2/hydroquinone (HQ) system allowed the construction of a linear calibration plot for RANKL standards between 10.4 and 1,000 pg mL−1 with a LOD of 3.1 pg mL−1. The determination implied simple handling protocols and short assay times. The developed immunosensor was applied to the determination of RANKL in human serum of patients diagnosed with colorectal cancer (CRC) or rheumatoid arthritis (RA). Each determination required only 5 μL of undiluted serum and took less than 2 h counting since the preparation of blocked‐bCAb‐Strep/p‐ABA‐SPCE.

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Study of the osteoprotegerin/receptor activator of nuclear factor-kB ligand system association with inflammation and atherosclerosis in systemic sclerosis

Abstract: In cardiovascular risks, OPG serum level might increase as a preventive compensatory mechanism to neutralize the RANKL level increment. The determination of the OPG-RANKL system is a diagnostic indicator for the intensity of vascular calcification and atherosclerosis in SSc patients.

Cited by 18 publications

References 35 publications

The Relationship between Serum and Gene Expression Levels of RANK, RANKL and Osteoprotegerin Inflammatory Pathway with Unstable Angina: A Case-control Study

The Relationship between Serum and Gene Expression Levels of RANK, RANKL and Osteoprotegerin Inflammatory Pathway with Unstable Angina: A Case-control Study

The Role of Osteoprotegerin System /Rankl/Rank in Pathogenesis of Aortic Stenosis

Carbon/Inorganic Hybrid Nanoarchitectures as Carriers for Signaling Elements in Electrochemical Immunosensors: First Biosensor for the Determination of the Inflammatory and Metastatic Processes Biomarker RANK‐ligand

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