Study of the peptide length and amino acid specific substitution in the antigenic activity of the chimeric synthetic peptides, containing the p19 core and gp46 envelope proteins of the HTLV-I virus

Marín, Milenen Hernández; Rodríguez-Tanty, Chryslaine; Higginson-Clarke, David; Bocalandro, Yadaris Márquez; Peña, Lilliam Pozo

doi:10.1016/j.bbrc.2005.08.207

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Selection of antigenic regions and plasmid constructs. The selection of antigenic regions of HTLV-1/2 structural proteins was based on epitope mapping studies reported in the literature (Table 1) (42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59). The coding sequences of the genetic constructs were acquired from GenScript (Piscataway, NJ, TABLE 1 Immunogenic regions of HTLV-1/2 structural proteins reported in the literature *Regions are according to the cited reference(s).…”

Section: Methodsmentioning

confidence: 99%

Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients

et al. 2021

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HTLV-1 can cause life-threatening diseases for which there are no effective treatments. Prevention of HTLV-1 infection requires massive testing of pregnant women, blood for transfusion and, organs for transplantation as well as safe sex. In this context, serological assays are widely used for monitoring HTLV-1 infections. Despite the necessity of recombinant antigens to compose serological tests, there is little information available on procedures to produce recombinant HTLV1/2 antigens for serological diagnostic purposes. In this work, we tested a series of genetic constructions to select those more amenable for production in bacterial systems. To overcome the constraints to express sections of viral envelope proteins in bacteria, we have used the p24 segment of the gag protein as a scaffold to display the immunogenic regions of gp46 and gp21. Nine recombinant antigenic proteins derived from HTLV-1 and five derived from HTLV-2 were successfully purified. The HTLV-1 antigens showed high efficiency in discriminating HTLV-positive from HTLV-negative samples using ELISA. Interestingly, HTLV-1-positive samples showed a high level of cross-reaction with HTLV-2 antigens. This finding is explained by the high sequence conservation between the structural proteins of these two highly related viruses. In summary, the results presented in this work provide a detailed description of the methods used to produce recombinant HTLV-1 and HTLV-2 antigens and demonstrate that the HTLV-1 antigens show strong potential for serological diagnosis of HTLV1 infections.

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Section: Methodsmentioning

confidence: 99%

Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients

et al. 2021

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“…Diversas tentativas para a expressão de proteínas do HIV-1 e HTLV-1 já foram realizadas por outros grupos de pesquisa e muitos deles conseguiram expressar as proteínas p24, gp41, gp120 e p19 usando diferentes sistemas de expressão, linhagens de bactérias, vetores e estratégias de clonagem. Como por exemplo, a expressão das proteínas p19, p24 e gp41 individualmente (RAVANSHAD et al, 2006;SATO et al, 1992;SOUTSCHEK;HOFLACHER;MOTZ, 1990;MARIN et al, 2005) ou a fusão de fragmentos de gp41 e gp120 (SOHN et al,1996). Portanto, mais experimentos e tentativas ainda devem ser realizadas para obtenção da expressão dessas quatro proteínas.…”

Section: Discussionunclassified

“…A expressão das proteínas recombinantes gp21, p24 e gp46 já foi realizada por outros grupos de pesquisa, porém as metodologias e os insumos para desenvolvimento de kits diagnósticos mais sensíveis e específicos devem ser sempre aprimorados (ABBASZADEGAN et al, 2008;COATES et al, 1990;KIYOKAWA et al, 1984;LILLEHOJ et al, 1990;MARIN et al, 2005;VARMA et al, 1995). A expressão da proteína gp46 recombinante, por exemplo, ocorreu de forma bem sucedida e o seu reconhecimento de forma específica pelos soros de indivíduos com HTLV-1 é um resultado importante e pode ser interessante para o melhoramento de produtos que já estão no mercado.…”

Section: Discussionunclassified

Clonagem de fragmentos dos genes gag e env do HIV-1 e HTLV-1, expressão em Escherichia coli das proteínas gp21, p24 e gp46 do HTLV-1 e imunodetecção

Davì¹

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Chimeric synthetic peptides as antigens for detection of antibodies to Trypanosoma cruzi

Marín¹,

Spengler²,

Martínez³

et al. 2006

Biochemical and Biophysical Research Communications

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Study of the peptide length and amino acid specific substitution in the antigenic activity of the chimeric synthetic peptides, containing the p19 core and gp46 envelope proteins of the HTLV-I virus

Cited by 4 publications

References 9 publications

Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients

Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients

Clonagem de fragmentos dos genes gag e env do HIV-1 e HTLV-1, expressão em Escherichia coli das proteínas gp21, p24 e gp46 do HTLV-1 e imunodetecção

Chimeric synthetic peptides as antigens for detection of antibodies to Trypanosoma cruzi

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