Study of the Polymorphism of Leptin Gene and Its Association with some Growth Traits in Lori Bakhtiari and Lori Bakhtiari-Afshari Croessbreed Sheep

Abstract

‫س‬ ‫دسیبفز:‬ ‫بسیخ‬ 21 / 8 / 96 ‫دزیشؽ:‬ ‫سبسیخ‬ 26 / 4 / 97 ‫چکیذُ‬ ‫پصٍّص‬ ‫ّذف‬ ‫حاضر‬ ، ‫از‬ ‫استفادُ‬ ‫با‬ ‫لپتیي‬ ‫شى‬ ‫ضکلی‬ ‫چٌذ‬ ‫هطالعِ‬ ‫تکٌیک‬ PCR-SSCP ‫آهیختِ‬ ٍ ‫بختیاری‬ ‫لری‬ ‫ًصاد‬ ‫گَسفٌذ‬ ‫در‬ ‫بختیاری‬ ‫لری‬ -‫صفات‬ ‫با‬ ‫شًَتیپی‬ ‫الگَّای‬ ‫ارتباط‬ ‫بررسی‬ ٍ ‫افطاری‬ ‫ر‬ ‫گَس‬ ‫در‬ ‫ضذ‬ ‫بختیاری‬ ‫لری‬ ‫فٌذاى‬ ‫بَد‬ . ‫از‬ 58 ‫ًصاد‬ ‫گَسفٌذ‬ ‫راض‬ ‫بختیاری‬ ‫لری‬ ‫ًر‬ ٍ ‫هادُ‬ ٍ ‫ضْرکرد‬ ‫ضَلی‬ ‫ایستگاُ‬ ‫در‬ ‫هَخَد‬ 42 ‫بختیاری‬ ‫لری‬ ‫آهیختِ‬ ‫ًصاد‬ ‫گَسفٌذ‬ ‫راض‬ -‫افطاری‬ ‫از‬ ‫رٍستاّای‬ ‫آهذ.‬ ‫عول‬ ‫بِ‬ ‫خًَگیری‬ ‫ضْرکرد‬ ‫استخراج‬ DNA ‫گرفت‬ ‫صَرت‬ ‫سیٌاشى‬ ‫ضرکت‬ ‫کیت‬ ‫با‬ . ‫کویت‬ ٍ ‫کیفیت‬ ‫تعییي‬ DNA ‫آگا‬ ‫شل‬ ‫با‬ ٍ ‫رز‬ ‫قطعِ‬ ‫تکثیر‬ ‫ضذ.‬ ‫اًدام‬ ‫اسپکترٍفتَهتری‬ 275 ‫اگسٍى‬ ‫بازی‬ ‫خفت‬ 3 ‫صَرت‬ ‫پلیوراز‬ ‫ای‬ ‫زًدیرُ‬ ‫ٍاکٌص‬ ‫از‬ ‫استفادُ‬ ‫با‬ ‫لپتیي‬ ‫شى‬ ‫ای‬ ‫رضتِ‬ ‫تک‬ ‫فضایی‬ ‫چٌذضکلی‬ ‫گرفت.‬ SSCP ‫هحصَالت‬ PCR ‫بذست‬ ‫ًقرُ‬ ‫ًیترات‬ ‫آهیسی‬ ‫رًگ‬ ٍ ‫آهیذ‬ ‫اکریل‬ ‫پلی‬ ‫شل‬ ‫از‬ ‫استفادُ‬ ‫با‬ ‫گَسفٌذا‬ ‫برای‬ ‫باًذی‬ ‫الگَی‬ ‫ّطت‬ ‫آهذ.‬ ‫ػبسی‬ ‫طجیؼی‬ ‫ٔٙبثغ‬ ٚ ‫وـبٚسصی‬ ‫ػّْٛ‬ ‫دا٘ـٍبٜ‬ ‫دأی‬ ‫سِٛیذار‬ ‫دظٚٞـٟبی‬ ‫ثخشیبسی‬ ‫ِشی‬ ‫٘ظاد‬ ‫ٌٛػفٙذاٖ‬ ‫دس‬ ‫سؿذ‬ ‫كفبر‬ ‫ثشخی‬ ‫ثب‬ ‫آٖ‬ ‫اسسجبط‬ ٚ ‫ِذشیٗ‬ ‫طٖ‬ ‫ؿىّی‬ ‫چٙذ‬ ‫ثشسػی‬ . AbstractThe purpose of this study was evaluation of leptin gene polymorphism by PCR-SSCP and its relationship with some growth traits in Lori Bakhtiari and crossbred of Lori Bakhtiari-Afshari sheep. Blood samples were collected from sheep (male and female) of Lori-Bakhtiari in Shahr-e-Kord Sholi station and sheep (male and female) of Lori Bakhtiari-Afshari crossbreed from villages of Shahr-e-Kord. DNA was extracted, using extraction kit of Sinnagen co. Evaluation of quality and quantity of DNA was performed using agarose gel electrophoresis and spectrophotometry. Polymerase chain reaction (PCR) was conducted to amplify bp fragment of exon of leptin gene. Then single strand conformation polymorphism (SSCP) of PCR products was performed and genotypic patterns were obtained using acrylamid gel and silver staining. For leptin gene in Lori bakhtiari sheep, band patterns including L to L and for crossbreeds sheep, band patterns including L to L were obtained. There was a high polymorphism in exon of the leptin gene in both breeds. Also results of means comparison showed that the leptin gene was significantly associated with weight at and months.