Study of the presence of Rhizobium bacteria which were inserted into the cell tissue of soybean seed using vacuum technology

Abstract

Rhizobium bacteria was known well as the bacteria which has potential on fixing nitrogen from the air and its widely used as biofertilizer. So far, the inoculants normally consist of Rhizobium bacteria which was packed in carrier material such as peat soil and applied to crop seed by mixing the seed with Rhizobium inoculant. This technique Rhizobium inoculant was not efficient anymore since much of bacteria cell was falling down, it did not completely sticked on the surface of seed. So this condition, resulted of un-effective function of the bacteria on running the nitrogen fixing process. To solve the problem, effort has been made to insert the bacteria inside the seed tissue using simple technology. This technology was developed for the target of symbiosis living between the bacteria and plants through optimal nitrogen fixing process. The purpose of this study is to confirm the presence of Rhizobium bacteria. That has been inserted into the soy bean seed using simple vacuum technology. The present of Rhizobium bacteria inside the seed tissue was observed from the preserved slices of seed tissue under amicroscope. The preserved slices of soybean seed tissue shown that the Rhizobium bacteria cell were able to reach the inside of seed tissue and fulfill the air space of palisade tissue.

Consideraciones sobre el uso de biofertilizantes como alternativa agro- biotecnológica sostenible para la seguridad alimentaria en México

“…Las especies de Rhizobium, además de fijar nitrógeno atmosférico, incrementan el crecimiento, rendimiento y número de nódulos por raíz y movilizan fósforo (Saharan y Nehra, 2011). Las investigaciones recientes en este género bacteriano como promotor del crecimiento vegetal se enfocan en analizar su efecto sobre i) la estructura de las comunidades microbianas asociadas a la raíz (Jha et al, 2020); ii) desarrollar biofertlizantes para múltiples cultivos de leguminosas (Passricha et al, 2020); iii) introducir células de Rhizobium dentro de semillas mediante tecnología de vació para evitar pérdidas del inóculo (Lekatompessy et al, 2020); y iv) evaluar el efecto de la co-inoculación de Rhizobium y esporas de endomicorrizas (Kiuk et al, 2019).…”