Study of the probiotic potential and evaluation of the survival rate of Lactiplantibacillus plantarum lyophilized as a function of cryoprotectant

Abstract

The benefits of probiotics for the improvement of animal health status have been of great interest in recent years. For this reason, in this study was aimed at assessing a strain with probiotic potential to be added to the feed. Therefore, the objective of this trial is to use a strain with probiotic potential isolated from the intestinal microbiota of Helix aspersa Müller to subsequently add it to the feed of this species to improve its health status. So, the strain is characterized, and its probiotic potential is demonstrated. Finally, with the aim of preserving the probiotic strain by freeze-drying so that it can later be added to the feed, different cryoprotectants were studied that could give it a higher survival rate over time. The cryoprotectant that gives the best result with strain survival rate is trehalose 15%.

The Impact of Formulation and Freeze Drying on the Properties and Performance of Freeze-Dried Limosilactobacillus reuteri R2LC

“…Type of sugar was the only factor that had a significant effect on all three quality attributes (Tables 6-8) and sucrose performed better overall than trehalose (Figures 7-9). Contrary to this, there are several publications that describe that trehalose provides a better protection of freeze-dried organisms and proteins than sucrose [39][40][41][42][43]. The reason for this discrepancy is not known, but it may be an effect specific to L. reuteri or the strain used (R2LC).…”

Investigate the impact of probiotics of lactic acid bacteria obtained from various local sources on some pathogenic bacteria

“…With parental permission, feces from healthy infants also including artisanal yogurt and cheese were analyzed for Lactobacillus (13). provided a detailed account of the isolation procedure, which was followed with a few tweaks here and there.…”

Screening of Lactiplantibacillus plantarum 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying

“…The hydroxyl groups contained in trehalose and sucrose in the PAs can replace the lost water, form hydrogen bonds with the phosphate and protein polar groups in the LAB cell membrane, and wrap on the protein surface in the form of a "hydration membrane", thereby protecting the integrity of the LAB cell membrane and protein structure and function during the VFD [38,39]; and at the same time, a glassy layer is formed on the surface of the LAB cell to wrap the biological structure, limiting the intramolecular mobility of the protein and protecting the function of the surface proteins of LAB [40]. In addition, sodium glutamate in the PAs can also form hydrogen bonds with the polar groups of LAB cell surface proteins so as to maintain the structural and functional integrity of LAB surface proteins [30].…”