Study of the Relationship between Changes in Lactic Acid Bacterial Cell Components and Stimulation of IL-12 Production under Salt-Stressed Conditions

Abstract

One hundred and seventeen strains of plant origin lactic acid bacteria were observed to have interleukin (IL)-12 production-inducing activities using mouse peritoneal macrophages. Pediococcus pentosaceus (KKM122) was chosen for its stable and strong IL-12 production-inducing activity. There was no significant difference in IL-12 activity induced by the KKM122 strain grown in culture conditions of 0% and 6% NaCl. The cell wall components of cells grown in 6% salt condition, however, significantly induced lower IL-12 production as compared with those of cells grown in 0% salt condition. Cell wall components enhanced IL-12 activity by removing cytoplasmic components when KKM122 strain was cultured in 0% salt condition. The immunoenhancing factor was mainly present in the cell wall components. IL-12 production-inducing activities were dependent on both the amount of bacterial cytoplasmic components and the structure of the cell wall components under the NaCl concentration in the culture medium.

Immuno-Modulatory Effects of Bacteriocin-Producing Pediococcus pentosaceus JWS 939 in Mice

“…P. pentosaceus also has immunomodulatory effects. Earlier studies reported that P. pentosaceus induces interferon-γ and interleukin (IL)-12, and suppresses IL-4 in mouse spleen cells or peritoneal macrophages (Igarashi, 2010;Jonganurakkun et al, 2008).…”