Study of tissue culture and in vitro organogenesis of Scutellaria bornmuelleri using benzylaminopurine, lsopentenyl adenine and thidiazuron

High-frequency adventitious shoot organogenesis from in vitro stem explants of Scutellaria araxensis Grossh

“…Conversely, a recent study conducted by Gharari et al (2021b) revealed that the use of BAP combined with TDZ in half-strength MS medium has a stronger effect on the percentage (100%) of Scutellaria bornmuelleri shoot organogenesis than TDZ when applied alone (93.3%) (Gharari et al, 2021b); thus, these findings support the result of the present study. A synergistic effect between TDZ and BAP was observed in S. bornmuelleri organogenesis (Gharari et al, 2021b).…”

“…Scutellaria is a widely distributed, subcosmopolitan genus comprising around 360 species with several therapeutic properties such as antitumor, hepatoprotective, antioxidant, antiinflammatory, anticonvulsant, antibacterial, and antiviral activities (Shang et al, 2010). Although there are a few reports on in vitro organogenesis and micro-propagation of Scutellaria species (Trivedi et al, 2011;Zakaria et al, 2020;Gharari et al, 2021b;Irvin et al, 2021), there is no report on the in vitro regeneration and propagation of S. araxensis . Accordingly, the present study was conducted to establish an efficient in vitro shoot organogenesis and plant regeneration protocol through multiple shoot induction from three types of explants, namely stem, petiole, and leaf of S. araxensis, by using different concentrations and combinations of BAP with TDZ, NAA, and IBA.…”

“…Half-strength MS medium supplemented with 0.5 mg/l gibberellic acid (GA 3 ) was used for shoot elongation and multiple shoot production. Root induction was performed in half-strength MS medium enriched with 0.5 mg/l IBA (Gharari et al, 2021b ). The pH of the medium was adjusted to 5.7–5.8 before the addition of agar (0.8% w/v), and the medium was then sterilized for 15 min at 121°C.…”

“…The obtained induced shoots were excised and transferred into half-strength solid MS medium supplemented with 0.5 mg/l IBA in a growth chamber maintained at 25± 2°C, 85% relative humidity, and 16 h photoperiod (Gharari et al, 2021b ). After 4 weeks, the rooted plantlets were removed from the medium and gently washed with sterile distilled water to remove traces of the adhered medium.…”

Shoot organogenesis and plant regeneration from leaf and petiole explants of Corydalis saxicola Bunting

“…Ultimately, while shoot organogenesis was possible from both leaf and petiole explants via an indirect callus-mediated stage, the most effective explant was in vitro leaves. This was also shown to be effective explants for the micropropagation of other rare or medicinal plants, such as Metabriggsia ovalifolia (Ma et al 2011), Scutellaria bornmuelleri (Gharari et al 2021), and Heliotropium foertherianum (Yu et al 2022).…”