Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients

Brugnon, Florence; Assche, E. Van; Verheyen, Greta; Sion, Benoı̂t; Boucher, D; Pouly, Jean Luc; Janny, L.; Devroey, Paul; Liebaers, Ingeborg; Steirteghem, André Van

doi:10.1093/humrep/dei401

Cited by 70 publications

(66 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Negative correlation has been associated between fertilization results with the presence of high levels of sperm DNA fragmentation [9,[36][37][38][39]. However, if the type and extent of DNA damage can be balanced by the reparative ability of the oocyte, it is possible to achieve fertilization even in the presence of elevated sperm DNA fragmentation rates [10,40,41].…”

Section: Discussionmentioning

confidence: 99%

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

Ferreyra¹

2014

JFIV Reprod Med Genet

View full text Add to dashboard Cite

Purpose: DNA fragmentation in spermatozoa has been associated with poor semen quality, low fertilization rates, impaired preimplantation development, and high miscarriage rates. The objective of this study was to evaluate the effects of the use of non-apoptotic MACS-selected spermatozoa in ICSI cycles, compared to those ICSI cycles in patients with normal sperm DNA fragmentation.Methods: A total of 134 cycles of ICSI were included in the study. The study group consisted of patients with high DNA fragmentation and their spermatozoa were selected by MACS (n=57); and the control group, of patients with normal DNA fragmentation and their spermatozoa were selected by classic morphological characteristics. The fertilization rate, cleavage rate, embryo quality, pregnancy rate (PR), implantation rate (IR) and miscarriage rate (MR) were compared between groups.Results: There was no difference in the fertilization rate (74.5 and 76.5%), cleavage rate and good embryo quality at Day 3 (98.3 and 89.1%; 88.4 and 83.7%) or blastocyst formation rate (50.8 and 41.1%) for the study and control groups, respectively. PRs, IRs and MRs were similar for the study group compared to the control group (PR: 63.2 versus 45.5%; IR: 37.4 versus 28.1%; MR: 8.3 versus 17.1). The women were distributed into three groups: <35 years, 35-39 years and ≥ 40 years. Pregnancy, implantation and miscarriage rates were similar in the three evaluated groups (P: NS). Conclusions:By selecting non-apoptotic sperm by MACS, we can achieve very acceptable pregnancy and implantation rates; being a good option for couples with high sperm DNA fragmentation and repeated assisted reproduction failures. Citation: García-Ferreyra J, Villegas L, Romero R, Zavala P, Hilario R, et al. (2014) High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation. JFIV Reprod Med Genet 3: 133. doi:10.4172/2375-4508.1000133 Several studies reported an improvement in fertilization rates [15,16] and embryo quality [17][18][19] because the best sperms were selected using MACS compared to standard selection methods. On the other hand, Gil et al. [20] showed that the implantation and miscarriage rated did not vary between MACS or standard sperm selection methods; however, they did observe an improvement in pregnancy rates. High Pregnancy and Implantation Rates CanIn this study, we evaluated the effects of using MACS as a sperm selection technique in patients with high DNA fragmentation compared with patients with normal DNA fragmentation and determine the efficacy of such technique in the hopes of improving ART success rates. Materials and Methods PatientsIn this study, we analyzed 134 cycles of ICSI that were done between March 2012 and April 2014 at FERTILAB Laboratory of Assisted Reproduction (Lima, Peru), all approved by the Institutional Review Board (IRB) and the corresponding Ethics Committee. Written consent was obtained from all patients and their...

show abstract

Section: Discussionmentioning

confidence: 99%

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

Ferreyra¹

2014

JFIV Reprod Med Genet

View full text Add to dashboard Cite

show abstract

“…Regarding sperm apoptosis, Brugnon et al [18] found phosphatidylserine externalization, a typical marker of apoptosis, and significantly higher DNA frag mentation rates in ejaculated spermatozoa from translo cation carriers than in spermatozoa from donors.…”

Section: Discussionmentioning

confidence: 99%

18, X, Y aneuploidies and transmission electron microscopy studies in spermatozoa from five carriers of different reciprocal translocations

Moretti¹,

Pascarelli²,

Giannerini³

et al. 2009

Asian J Androl

View full text Add to dashboard Cite

We analysed ejaculated spermatozoa from five infertile men with different balanced reciprocal translocations to contribute to the study of meiotic segregation of chromosomes 18, X and Y and also to evaluate sperm morphology by transmission electron microscopy (TEM) analysis. Conventional lymphocyte karyotype analyses highlighted dif ferent reciprocal balanced translocations: t(12;13), t(4;9), t(X;8), t(8;10) and t(3;16). Semen analysis was performed by light and TEM. Fluorescence in situ hybridization was performed directly on sperm nuclei using centromeric probes for chromosomes 18, X and Y. The carriers of the balanced reciprocal translocations considered in the pres ent study showed a very similar pattern of sperm pathologies: diffused presence of apoptosis and immaturity. All patients showed meiotic segregation derangements, highlighted by the presence of sperm diploidies and sex chro mosome disomies particularly related to the failure of the first meiotic division. However, an increased incidence of chromosome 18 aneuploidy was detected in spermatozoa from t(X;8) and t(8;10) carriers. We have also reported values from sex chromosomes such as t(X;8), although the X chromosome was involved in translocation. Since pa tients with reciprocal translocations and spermatogenetic impairment are candidates for intracytoplasmic sperm in jection cycles, the study of sperm parameters, and particularly of the level of aneuploidy rates, would provide better information for couples at risk and would contribute to the data in the literature for a better understanding of the ef fects of chromosomal rearrangement on the whole meiotic process and, in particular, on chromosomes not involved in translocation.

show abstract

“…It can be argued that this association between chromosomal imbalance and DNA fragmentation has only been shown in ten patients so far (four by Perrin et al in [18], and six in the present study). However, since the association between DNA fragmentation, apoptosis markers, and chromosomal rearrangements has been evidenced in multiple studies [6,7,17], it is possible than in most if not all of the patients analyzed in those studies, DNA fragmentation is more often found among unbalanced spermatozoa. Therefore, it is likely that our population of six patients is representative of the chromosomal events in sperm from translocations carriers.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on the subject stated that chromosomal rearrangements were associated with elevated global levels of DNA fragmentation and apoptosis [6,7,17]. However those studies were carried out in reproductive medicine centers, and most subjects initially consulted for infertility.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that patients carrying a chromosomal rearrangement have significantly higher global sperm DNA fragmentation levels and expression of apoptosis markers than subjects with a normal karyotype [6,7,18]. However, in these studies, spermatozoa have been analyzed as a whole and no relationship between the level of DNA fragmentation and a particular nuclear content was evidenced.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous cell by cell study of both DNA fragmentation and chromosomal segregation in spermatozoa from chromosomal rearrangement carriers

Pyram

Pollet-Villard

Hyon

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Balanced chromosomal translocations are found in one out of 500 subjects in the general population. They usually do not carry any phenotypic consequences, except for possible infertility and for the production of unbalanced gametes leading to spontaneous abortions or chromosomal syndromes in the offspring. An association between chromosomal rearrangements and increased apoptosis markers has been demonstrated on a global scale in sperm samples of translocation and inversion carriers. In order to specify which kind of sperm cells is subject to an increased apoptosis process, this present study was aimed to analyse both chromosomal segregation and DNA fragmentation, sperm cell by sperm cell. Methods Six patients carrying a chromosomal rearrangement (three reciprocal translocations, two Robertsonian translocations, and one chromosomal pericentric inversion) were included in a retrospective manner. Both DNA fragmentation and chromosomal segregation in spermatozoa were evaluated simultaneously using a modified TUNEL assay associated with FISH. Two thousand spermatozoa were analysed for each patient. ResultsWe showed a higher proportion of spermatozoa with fragmented DNA among the unbalanced sperm cells, compared to the balanced ones, in all six patients. Conclusions These results suggest an increased fragility of unbalanced spermatozoa to exogenous fragmentation factors. The exact mechanisms of those processes remain to be elucidated.

show abstract

Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients

Cited by 70 publications

References 50 publications

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

18, X, Y aneuploidies and transmission electron microscopy studies in spermatozoa from five carriers of different reciprocal translocations

Simultaneous cell by cell study of both DNA fragmentation and chromosomal segregation in spermatozoa from chromosomal rearrangement carriers

Contact Info

Product

Resources

About