Study of vaginal microbiota of Holstein cows submitted to an estrus synchronization protocol with the use of intravaginal progesterone device

Quadros, Daniel Lazzari; Zanella, Ricardo; Bondan, Carlos; Zanella, Giovana Ciacci; Facioli, Fernanda Luiza; Silva, Arthur Nery da; Zanella, Eraldo Lourenso

doi:10.1016/j.rvsc.2020.03.027

Cited by 18 publications

(8 citation statements)

References 28 publications

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“…In this way, we can investigate not only if the success of AI is affected by microbial composition in the vagina but also the effect of the progestogen (and associated treatments) on the vaginal microbiota. Although recently a body of knowledge has been developed to elucidate the relationship between vaginal microbiome and fertility, all the studies have been carried out through the analysis of the hypervariable regions of the 16S RNA gene (e.g., Swartz et al, 2014 and Serrano et al, 2020 in sheep; Laguardia-Nascimento et al, 2015 in cattle; Mahalingam et al, 2019 in buffalo; Giannatasio-Ferraz et al, 2019 and Quadros et al, 2020 in cows; Rhoades et al, 2021 in macaques).…”

Section: Discussionmentioning

confidence: 99%

Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome

et al. 2023

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IntroductionThe low pregnancy rate by artificial insemination in sheep represents a fundamental challenge for breeding programs. In this species, oestrus synchronization is carried out by manipulating hormonal regimens through the insertion of progestogen intravaginal devices. This reproductive strategy may alter the vaginal microbiota affecting the artificial insemination outcome.MethodsIn this study, we analyzed the vaginal microbiome of 94 vaginal swabs collected from 47 ewes with alternative treatments applied to the progesterone-releasing intravaginal devices (probiotic, maltodextrin, antibiotic and control), in two sample periods (before placing and after removing the devices). To our knowledge, this is the first study using nanopore-based metagenome sequencing for vaginal microbiome characterization in livestock.ResultsOur results revealed a significant lower abundance of the genera Oenococcus (Firmicutes) and Neisseria (Proteobacteria) in pregnant compared to non-pregnant ewes. We also detected a significant lower abundance of Campylobacter in the group of samples treated with the probiotic.DiscussionAlthough the use of probiotics represents a promising practice to improve insemination results, the election of the suitable species and concentration requires further investigation. In addition, the use of progestogen in the synchronization devices seemed to increase the alpha-diversity and decrease the abundance of harmful microorganisms belonging to Gammaproteobacteria and Fusobacteriia classes, suggesting a beneficial effect of their use.

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Section: Discussionmentioning

confidence: 99%

Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome

et al. 2023

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“…In Holstein Friesian cattle, the most ubiquitous dairy breed in North Africa, Europe, and the USA, the vaginal microbiome was predominately composed of Firmicutes , Tenericutes , Proteobacteria , and Bacteriodetes phyla. Other bacteria were detected in smaller quantities such as Actinobacteria and Spirochaetae [ 48 ].…”

Section: Factors Affecting Genital Microbiome Diversitymentioning

confidence: 99%

A Review of the Diversity of the Genital Tract Microbiome and Implications for Fertility of Cattle

Adnane

Chapwanya

2022

Animals

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Cattle have a genital microbiome that is established early in life, even before calving. Microbial influx into the reproductive system of cows, during calving or mating, is unavoidable and is likely to alter the commensal microflora composition. It is now well established that a commensal endometrial flora is largely responsible for the overall fertility of cows. These microbes are important for maintenance of structural integrity of the genital mucosal barrier, immunomodulation, and protection against pathogens. Further, the genital microbiome functions in the semiochemical communication between a male and female. An optimal balance between the abundance and diversity of the microbiome is essential to promote female genital tract health. Disruption of this balance leads to dysbiosis and genital diseases and perturbed fertility. As part of the global strategy of One World, One Health, there is a need to reduce antibiotic use in animals. This area of research has the potential to expand the knowledge about the nexus between the endometrial microbiome and fertility including being probiotic in different species.

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“…Several authors reported Firmicutes as the most abundant phylum in ewes ( Serrano et al, 2020 ; Koester et al, 2021 ; Greenwood et al, 2022 ; Reinoso-Peláez et al, 2023 ) and cattle ( Laguardia-Nascimento et al, 2015 ; Bicalho et al, 2017 ; Giannattasio-Ferraz et al, 2019 ; Miranda-CasoLuengo et al, 2019 ; Chen et al, 2020 ; Quadros et al, 2020 ). The results of our study are consistent with these previous studies as Firmicutes was always the predominant phylum except in Tpreg_NP.…”

Section: Discussionmentioning

confidence: 99%

Description of the vaginal microbiota in nulliparous ewes during natural mating and pregnancy: preliminary signs of the male preputial microbiota modulation

Barba,

Toquet,

García-Roselló

et al. 2024

Front. Microbiol.

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The vaginal microbiota plays a key role in animals’ health. Understanding its diversity and composition and associated changes occurring through the reproductive cycle represents valuable knowledge to disclose the mechanisms leading to dysbiosis and eventually to infection. Even if the human vaginal microbiota has been thoroughly studied, scarce research has been conducted on the vaginal microbiota of livestock. In this study, 16S rRNA gene-based sequencing was performed on vaginal samples of ten nulliparous ewes at three different sampling points: before the estrus synchronization protocol (T0), at the time of estrus before mating (Testrus), and the day of the pregnancy diagnosis (Tpreg). Preputial samples from the three males collected pre and post-mating were also analyzed. Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria were the most abundant phyla in vaginal samples. The most abundant genera were Porphyromonas, Anaerococcus, and Peptinophilius. Vaginal microbiota biodiversity decreased during pregnancy. Tenericutes (Ureaplasma spp.) increased significantly at Tpreg in both pregnant and non-pregnant ewes. Differences were observed between pregnant and non-pregnant ewes at Tpreg where pregnant ewes had a significantly higher abundance of Actinobacillus spp. and Ureaplasma spp. Ewes that were diagnosed with pregnancy at Tpreg showed a decreased abundance of gram-negative bacteria such as Bacteroidales, Campylobacterales, and Enterobacteriales. In addition, a significant decrease in the relative abundances of genera within Firmicutes, such as Alloicoccus (Lactobacillales), Atopostipes (Lactobacillales), and an uncultured bacteria W5053 from Family XI (Firmicutes, Clostridiales) was observed in non-pregnant ewes at Tpreg. The four most abundant phyla in the rams’ prepuce were the same as in the ewes’ vagina. The most abundant genus was Corynebacterium. No major differences were observed in the ram’s preputial microbiota between pre and post-mating samples. Nevertheless, the differences in the taxonomic composition of ewes’ vaginal microbiota between Testrus and Tpreg could be explained by the exposure to the preputial microbiota. This study offers new insights into the effects of several key steps of the ewe’s reproductive cycle such as estrus-synchronization protocol, mating, and pregnancy on ovine vaginal microbiota. The knowledge of the microbiota dynamics during the reproductive cycle can help improve the reproductive outcomes of dams by identifying biomarkers and putative probiotics.

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Study of vaginal microbiota of Holstein cows submitted to an estrus synchronization protocol with the use of intravaginal progesterone device

Cited by 18 publications

References 28 publications

Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome

Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome

A Review of the Diversity of the Genital Tract Microbiome and Implications for Fertility of Cattle

Description of the vaginal microbiota in nulliparous ewes during natural mating and pregnancy: preliminary signs of the male preputial microbiota modulation

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