Study on antioxidant activity of wheat bran extract microcapsules in vitro and in vivo

Abstract

Wheat bran extracts (WBE, mainly composed of feruloyl oligosaccharides (FOs)) have been approved by FDA in GRAS Notice. WBE was utilised in octenyl succinate anhydride-modified starch (OSA-starch)based microcapsules as wall materials to protect β-carotene (BC) from oxygen. The storage retention and antioxidant indexes in vivo and in vitro of microcapsules were determined. The results showed that these characteristics were positively related to the proportion of WBE in the wall material. The in vivo antioxidant indexes in mouse serum and liver were positively regulated at gavage dose around content in wheat grain (normal dose), while at a higher dosage, the in vivo free oxidative stress fell into disorder. The results indicated that WBE was a good component of wall material, which can improve the antioxidant environment during both storage and consumption. This experiment provides a theoretical basis for the industrial application of WBE in the field of microcapsules.

Antioxidant properties of wheat bran FOs prepared by Bacillus amyloliquefaciensIT‐45 fermentation

“…Sibhatu et al employed the same approach for ferulic acid production and got 46.17 mg FA per 100 g of brewery spent grains (Sibhatu et al, 2021). Gong et al and Yu et al adopted the same method for the quantification of FA content in wheat bran FOs prepared by enzyme-assisted extraction and the FA content were 39.24 and 41.25 mg/g, respectively (Gong et al, 2019;Yu et al, 2022). Therefore, it demonstrated that HPLC could be used to quantify FA content in FOs at first.…”

“…The FTIR findings revealed the formation of ester bonds between ferulic acid and saccharides. The distinctive FTIR profile of wheat bran FOs, achieved through B. amyloliquefaciens IT‐45 fermentation, exhibited similarities to the profile of wheat bran‐derived FOs by Aspergillus niger fermentation (Yu et al ., 2022).…”

“…and Yu et al . adopted the same method for the quantification of FA content in wheat bran FOs prepared by enzyme‐assisted extraction and the FA content were 39.24 and 41.25 mg/g, respectively (Gong et al ., 2019; Yu et al ., 2022). Therefore, it demonstrated that HPLC could be used to quantify FA content in FOs at first.…”

“…The ester bonds or ether bonds are formed between the carboxylic acid groups of ferulic acid and the side chain of α‐L‐arabinose xylan or the lignin monomers in the cell walls of Gramineae plants. Normally, FOs are obtained from hydrolyzing the cell walls of wheat bran, including microwave‐assisted extraction (Rose & Inglett, 2010), acid/alkali extraction (Lin et al ., 2014b), enzyme‐assisted extraction (Yuan et al ., 2006) and microbial fermentation assistance extraction (Yu et al ., 2022; Chen et al ., 2023). Physical technique required strict reaction time and temperature, and high energy consumption.…”