Study on cerebroprotective actions of Clerodendron glandulosum leaves extract against long term bilateral common carotid artery occlusion in rats

Surapaneni, Saritha; Prakash, T.; Ansari, M. A.; Manjunath, Pramod; Kotresha, D.; Goli, Divakar

doi:10.1016/j.biopha.2016.02.029

Cited by 16 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vehicle‐treated animals showed considerably ( P < 0.05) higher ELT on day 6 and lower TSTQ on day 7 when compared to the sham group, which indicated the detrimental effects of I‐R on cognitive functions of mice (Table 3 and Figure 2A). These effects are in harmony with earlier studies showing the cognitive impairment in animals after BCCAO‐induced cerebral injury (Singh et al., 2017; Surapaneni et al., 2016). Among the groups treated with EF and AF, EF‐treated group (85 and 170 mg/kg) showed a marked ( P < 0.05) decrease in ELT on day 6 and an increase in TSTQ in comparison to the vehicle‐treated animals, which indicated its cognitive enhancement effects against BCCAO.…”

Section: Resultssupporting

confidence: 91%

Isolation and characterization of components responsible for neuroprotective effects ofAllium cepaouter scale extract against ischemia reperfusion induced cerebral injury in mice

Singh

Shri

Krishan

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

The antioxidant‐mediated neuroprotective effect of Allium cepa outer scale extract (ACE) in mice with cerebral ischemia‐reperfusion (I‐R) injury was demonstrated in our earlier work. The current investigation aimed at establishing the bioactive component(s) responsible for this activity. Thus ACE was fractionated into ethyl acetate (EF) and aqueous (AF) fractions. These fractions were evaluated against cerebral I‐R injury in mice. I‐R injury in mice was induced by bilateral common carotid artery occlusion followed by 24 hr reperfusion. Memory, sensorimotor functions, cerebral infarct size, and oxidative stress were measured to address the neuroprotective mechanism of test substances. EF showed marked improvement of memory and sensorimotor functions by reducing brain oxidative stress and infarct size in mice after I‐R injury. The bioactive EF was subjected to chromatographic (HPLC‐PDA, HPLC‐MS, preparative HPLC) and spectroscopic studies to isolate and identify the neuroprotective compounds. This lead to separation of three components, namely quercetin, quercetin 4′‐O‐glucoside, and the remaining fraction, from EF. The separated components were biologically evaluated. These components showed improvement in mice with I‐R injury. But, EF displayed more marked neuroprotective effects as compared to the isolated components. The distinct neuroprotective outcome of EF may be credited to the synergistic action of compounds present in EF. Further studies such as evaluation of neurotoxic effects and other possible neuroprotective mechanisms are required to develop EF as a neuroprotective drug.

show abstract

Section: Resultssupporting

confidence: 91%

Isolation and characterization of components responsible for neuroprotective effects ofAllium cepaouter scale extract against ischemia reperfusion induced cerebral injury in mice

Singh

Shri

Krishan

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…In a vehicle-treated group, significantly higher day 6 ELT and lower TSTQ was observed compared with sham animals indicating cognitive impairment effects of BCCAO in animals. These results are supported by previous reports showing BCCAO induced cognitive impairment in animals [35,54]. Treatment of animals with EF-L by IN route after BCCAO exhibited significantly lower day 6 ELT and higher TSTQ in comparison with the vehicle-treated group showing beneficial effects of EF-L against BCCAO in mice.…”

Section: Effect Of Ef-l Treatment On Cognitive Functionssupporting

confidence: 90%

“…The pathophysiology of I-R-induced neuronal injury involves a series of complex processes of which oxidative stress has been identified as a major cause of cerebral damage [57]. I-R injury is well documented to produce oxidative stress-mediated neurodegeneration by depleting endogenous antioxidants like reduced glutathione (GSH) and superoxide dismutase (SOD) activity and increasing lipid peroxidation [45,54,58]. In line with these reports, the present study showed a significant decrease in GSH levels and SOD activity and increase in TBARS levels (index of lipid peroxidation) in the vehicle-treated group in comparison with the sham group.…”

Section: Effect Of Ef-l Treatment On Brain Biochemical Parametersmentioning

confidence: 99%

Amelioration of ischaemia reperfusion-induced cerebral injury in mice by liposomes containing Allium cepa fraction administered intranasally

Singh

Krishan

Shri

2018

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Neuroprotection in ischaemic stroke prevents neuronal injury and subsequent death. Our earlier work revealed the neuroprotective effect of ethylacetate fraction (EF) obtained from Allium cepa outer scales in a mouse model of cerebral ischaemia-reperfusion (I-R) injury. The present study was designed to develop and optimize a liposomal delivery system for EF, along with its biological assessment. Thin film hydration method was used for the preparation of liposomal formulation. The prepared liposomes were optimized with respect to particle size, size distribution and encapsulation efficiency (EE) and characterised on the basis of zeta potential, in vitro release, morphology (TEM) and physical stability. The biological activity of the optimized liposomal formulation (EF-L; 8.5 mg/kg, intra-nasal) was evaluated after induction of cerebral injury in the experimental animals. Neuroprotective effects were assessed in terms of improvement of cognitive/sensorimotor functions and reduction of cerebral infarct size and brain oxidative stress. EF-L (particle size of 204.93 ± 7.96 nm; EE of 88.02 ± 2.09%; zeta potential of -20.8 ± 1.24 mV) showed controlled release pattern; spherical shape and were physically stable for 60 days at 4 C. Intra-nasal administration of EF-L produced significant neuroprotection in mice at 1/10th the oral dose. Thus, EF-L may be developed as a neuroprotective formulation.

show abstract

“…In vitro [215] Neuronal death prevention In vivo [216] Anti-convulsant In vivo [217] Inhibition of monoamine oxidase (MAO), AChE, and BChE activities In vitro [218] Antioxidative insult In vivo [219] Cerebroprotective action In vivo [220] Prevention of okadaic-acid-induced injury by MAPK and PI3K/Akt/GSK3β signaling pathways In vitro [221] Reduction of oxaliplatin-induced oxidative stress in brain In vitro and in vivo [222] Table 2. Cont.…”

Section: Antioxidant and Ache Inhibitory Activitymentioning

confidence: 99%

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

Bhat

2021

Biology

View full text Add to dashboard Cite

Quercetin, a bioactive secondary metabolite, holds incredible importance in terms of bioactivities, which has been proved by in vivo and in vitro studies. The treatment of cardiovascular and neurological diseases by quercetin has been extensively investigated over the past decade. Quercetin is present naturally in appreciable amounts in fresh produce (fruits and vegetables). However, today, corresponding to the growing population and global demand for fresh fruits and vegetables, a paradigm shift and focus is laid towards exploring industrial food wastes and/or byproducts as a new resource to obtain bioactive compounds such as quercetin. Based on the available research reports over the last decade, quercetin has been suggested as a reliable therapeutic candidate for either treating or alleviating health issues, mainly those of cardiovascular and neurological diseases. In the present review, we have summarized some of the critical findings and hypotheses of quercetin from the available databases foreseeing its future use as a potential therapeutic agent to treat cardiovascular and neurological diseases. It is anticipated that this review will be a potential reference material for future research activities to be undertaken on quercetin obtained from fresh produce as well as their respective processing wastes/byproducts that rely on the circular concept.

show abstract

Study on cerebroprotective actions of Clerodendron glandulosum leaves extract against long term bilateral common carotid artery occlusion in rats

Cited by 16 publications

References 21 publications

Isolation and characterization of components responsible for neuroprotective effects ofAllium cepaouter scale extract against ischemia reperfusion induced cerebral injury in mice

Isolation and characterization of components responsible for neuroprotective effects ofAllium cepaouter scale extract against ischemia reperfusion induced cerebral injury in mice

Amelioration of ischaemia reperfusion-induced cerebral injury in mice by liposomes containing Allium cepa fraction administered intranasally

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

Contact Info

Product

Resources

About