Study on differential expression of 1-aminocyclopropane-1-carboxylic acid oxidase genes in table grape cv. Thompson Seedless

Abstract

International audienceAs a consequence of the non-climacteric status of grapes (Vitis vinifera), ethylene biosynthesis and signal transduction have scarcely been studied in this fruit. In spite this drawback, the available information suggests a role for ethylene in ripening grape berries. In this work, we report the identification of three homologous genes that encode 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), a key component of ethylene biosynthesis. A comparison of protein sequences revealed that all three VvACOs harbor a 2OG-Fe(II) oxygenase domain, which is typical of the ACO gene family; however, VvACO1 showed a higher amino acid sequence homology with VvACO2 than with VvACO3. The expression pattern of VvACOs and the effect of exogenous ethylene on their transcript accumulation were evaluated during table grape berry development in the "Thompson Seedless" cultivar. A peak in VvACO1 transcript accumulation levels was registered around veraison that was 4-fold higher than at harvest, and this peak was confirmed during a second season in grapes that were harvested from three different vineyards. An enhancement in ethylene production and VvACO genes transcript levels was observed in grapes sprayed with ethephon during berry development. However, VvACO1 transcripts reached the highest accumulation earlier than VvACO2 and VvACO3. Altogether, these data confirmed that ethylene may have a role in some aspects of the grape ripening process, and they also highlighted the potential use of some VvACO genes as molecular markers for identifying grape veraison stages in grapes

Expression Profiles and DNA-Binding Affinity of Five ERF Genes in Bunches of Vitis vinifera cv. Cardinal Treated with High Levels of CO2 at Low Temperature

“…Likewise, their results of the evolution of both ACC (1-aminocyclopropane-1-carboxylic acid) content and ACO (ACC oxidase) activity strongly support the increase in ethylene production in grapevines during the early stage of cold stress. Results obtained by Muñoz-Robredo et al (2013), confirmed that ethylene may have a role in some aspects of the grape ripening process. They also highlighted the potential use of VvACO1 , as a molecular marker for identifying grape veraison stages.…”

Proteome Profile of American Hybrid Grape cv. Blanc du Bois during Ripening Reveals Proteins Associated with Flavor Volatiles and Ethylene Production

“…Synthesis of ethylene biosynthesis begins with the conversion of methionine to S‐adenosylmethionine (SAM) by S‐adenosylmethionine synthase (SAMS) . SAM is then converted to 1‐amino syclopropane‐1‐carboxylic acid (ACC) by ACC synthase.…”

“…SAM is then converted to 1‐amino syclopropane‐1‐carboxylic acid (ACC) by ACC synthase. Further, ACC is converted to ethylene in the presence of oxygen by ACC oxidase which is a rate limiting step . SAM can also be demethylated to form S ‐adenosyl‐ l ‐homocysteine and catabolized to homocysteine and adenosine by action of SAHH.…”

Expression Patterns of ACS and ACO Gene Families and Ethylene Production in Rachis and Berry of Grapes

“…For instance, EjACS1 and EjACO1 are specifically expressed in loquat fruit (Jiang et al, 2011), and LeACO1 is specifically expressed in tomato fruit (Barry et al, 1996). In grapes, there are at least 10 VvACS and 3 VvACO genes (Munoz-Robredo et al, 2013;Xu and Wang, 2012). The highest expression of VvACO1 gene was concomitant with an ethylene peak around veraison (Chervin et al, 2008;Munoz-Robredo et al, 2013;Pech et al, 2008).…”

“…In grapes, there are at least 10 VvACS and 3 VvACO genes (Munoz-Robredo et al, 2013;Xu and Wang, 2012). The highest expression of VvACO1 gene was concomitant with an ethylene peak around veraison (Chervin et al, 2008;Munoz-Robredo et al, 2013;Pech et al, 2008). Moreover, there is little published data about the temporal and spatial expression of ACS and ACO genes in grapes.…”