Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics

Zhao, Rui; Farooq, Muhammad Umer; Wang, Li; Yang, Shuxu; Zheng, Ting; Ye, Xiaoying; Jia, Xiaomei; Zhu, Jianqing

doi:10.3390/biom9040130

Cited by 16 publications

(5 citation statements)

References 42 publications

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“…The association of methylated compounds (methyl salicylate and methyl heptanone) with Se-treated fruit may show how selenium affects tomato methyltransferases. In fact, in a proteomics study, Zeng et al [ 36 ] demonstrated that methyl transferases are among the differentially expressed proteins in naturally selenium-enriched rice.…”

Section: Resultsmentioning

confidence: 99%

Se-Enrichment Pattern, Composition, and Aroma Profile of Ripe Tomatoes after Sodium Selenate Foliar Spraying Performed at Different Plant Developmental Stages

Meucci¹,

Ширяев²,

Rosellini³

et al. 2021

Plants

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Foliar spray with selenium salts can be used to fortify tomatoes, but the results vary in relation to the Se concentration and the plant developmental stage. The effects of foliar spraying with sodium selenate at concentrations of 0, 1, and 1.5 mg Se L-1 at flowering and fruit immature green stage on Se accumulation and quality traits of tomatoes at ripening were investigated. Selenium accumulated up to 0.95 µg 100 g FW-1, with no significant difference between the two concentrations used in fruit of the first truss. The treatment performed at the flowering stage resulted in a higher selenium concentration compared to the immature green treatment in the fruit of the second truss. Cu, Zn, K, and Ca content was slightly modified by Se application, with no decrease in fruit quality. When applied at the immature green stage, Se reduced the incidence of blossom-end rot. A group of volatile organic compounds (2-phenylethyl alcohol, guaiacol, (E)-2-heptenal, 1-penten-3-one and (E)-2-pentenal), positively correlated with consumer liking and flavor intensity, increased following Se treatment. These findings indicate that foliar spraying, particularly if performed at flowering stage, is an efficient method to enrich tomatoes with Se, also resulting in positive changes in fruit aroma profile.

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Section: Resultsmentioning

confidence: 99%

Se-Enrichment Pattern, Composition, and Aroma Profile of Ripe Tomatoes after Sodium Selenate Foliar Spraying Performed at Different Plant Developmental Stages

Meucci¹,

Ширяев²,

Rosellini³

et al. 2021

Plants

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“…However, expression of the Aldh2 gene was not consistent with that of the protein. Many studies have reported that proteome and transcriptome results are not related or negatively correlated (Zeng et al 2019). Post‐transcriptional regulatory processes, such as alternative splicing, RNA processing and other processes, may affect the efficiency of translation (Lan et al 2011).…”

Section: Results and Discusionmentioning

confidence: 99%

“…The liquid chromatography‐tandem mass spectrometry data were optimized by series and then compared with the database to score the protein for protein identification (Zeng et al . 2019). The quantitative protein ratios, which require that a protein contains at least one unique peptide, were weighted and normalized by the median ratio in Mascot (Ma et al .…”

Section: Methodsmentioning

confidence: 99%

Responses of Phragmites australis to copper stress: A combined analysis of plant morphology, physiology and proteomics

Wang

et al. 2020

Plant Biol J

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Few relevant research attempts have been made to determine heavy metal resistance mechanisms of rhizomatous perennial plants. Thus, it is pertinent to investigate the physiological and biochemical changes in Phragmites australis under metal‐stressed conditions to facilitate the development of strategies to enhance copper (Cu) tolerance. We measured parameters related to plant growth and development, metal translocation and physiological responses of P. australis subjected to Cu stress. In addition, the differentially expressed proteins (DEP) were evaluated using the isobaric tag for relative and absolute quantification (iTRAQ) system. A large amount of copper accumulates in the roots of P.australis, but the growth parameters were not sensitive to Cu. However, the high concentration of Cu reduced the content of chlorophyll a and chlorophyll b, and the expression of important photosynthesis proteins PsbD, PsbO and PsaA were all down‐regulated, so photosynthesis was inhibited. In contrast, the content of ascorbic acid and proline both increased with the increase of copper stress. P.australis fixed a large amount of Cu in its roots, limiting the migration of Cu to other parts of the plant. Moreover, Cu stress can affect photosynthesis by inhibiting the activity of PSI, PSII and LHCII. In addition, P.australis synthesizes ascorbic acid through the D‐mannose/L‐galactose pathway, and synthesizes proline through the ornithine pathway. Ascorbic acid and proline can increase Cu tolerance and protect photosynthesis. These results provide a theoretical basis for understanding the tolerance and repair mechanisms of plants in response to heavy metal pollution.

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“…Interestingly, 77 targeted significant DAPs were screened further and classified into 10 sets comprising of actin, synthetases, hydrolases, tubulin, ligases, lyases, isomerases, heat shock proteins, oxidoreductases, and transferases. These findings indicated that active oxygen metabolism, anti-stress, anti-oxidation, amino acid, and carbohydrate metabolism of Se-enriched rice seedlings was higher as compared to non-Se-enriched plants ( Zeng et al, 2019 ).…”

Section: Seven-(omics)-based Approaches To Improve Toxic Metals/metalloids Tolerance In Plantsmentioning

confidence: 94%

“…Furthermore, various heat shock proteins (HSPs) were also identified as DAPs, which helped to cope with metal toxicity ( Zhang et al, 2019a ). Zeng et al (2019) performed quantitative proteomics (iTRAQ) to study differential protein expression in Se-enriched and non-Se-enriched rice seedlings. Overall, 3235 proteins were detected, of which 3,161 proteins were quantified from 401 DAPs.…”

Section: Seven-(omics)-based Approaches To Improve Toxic Metals/metalloids Tolerance In Plantsmentioning

confidence: 99%

Advances in “Omics” Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants

et al. 2022

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Food safety has emerged as a high-urgency matter for sustainable agricultural production. Toxic metal contamination of soil and water significantly affects agricultural productivity, which is further aggravated by extreme anthropogenic activities and modern agricultural practices, leaving food safety and human health at risk. In addition to reducing crop production, increased metals/metalloids toxicity also disturbs plants’ demand and supply equilibrium. Counterbalancing toxic metals/metalloids toxicity demands a better understanding of the complex mechanisms at physiological, biochemical, molecular, cellular, and plant level that may result in increased crop productivity. Consequently, plants have established different internal defense mechanisms to cope with the adverse effects of toxic metals/metalloids. Nevertheless, these internal defense mechanisms are not adequate to overwhelm the metals/metalloids toxicity. Plants produce several secondary messengers to trigger cell signaling, activating the numerous transcriptional responses correlated with plant defense. Therefore, the recent advances in omics approaches such as genomics, transcriptomics, proteomics, metabolomics, ionomics, miRNAomics, and phenomics have enabled the characterization of molecular regulators associated with toxic metal tolerance, which can be deployed for developing toxic metal tolerant plants. This review highlights various response strategies adopted by plants to tolerate toxic metals/metalloids toxicity, including physiological, biochemical, and molecular responses. A seven-(omics)-based design is summarized with scientific clues to reveal the stress-responsive genes, proteins, metabolites, miRNAs, trace elements, stress-inducible phenotypes, and metabolic pathways that could potentially help plants to cope up with metals/metalloids toxicity in the face of fluctuating environmental conditions. Finally, some bottlenecks and future directions have also been highlighted, which could enable sustainable agricultural production.

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Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics

Cited by 16 publications

References 42 publications

Se-Enrichment Pattern, Composition, and Aroma Profile of Ripe Tomatoes after Sodium Selenate Foliar Spraying Performed at Different Plant Developmental Stages

Se-Enrichment Pattern, Composition, and Aroma Profile of Ripe Tomatoes after Sodium Selenate Foliar Spraying Performed at Different Plant Developmental Stages

Responses of Phragmites australis to copper stress: A combined analysis of plant morphology, physiology and proteomics

Advances in “Omics” Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants

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