Study on Diversity of Sapodilla (Manilkara zapota) by Molecular Marker in the Special Region of Yogyakarta

Abstract

The objective of this research was to determine the diversity and relationship of sapodilla accession derived from districts in DIY based on DNA profiles. From the screening of 20 total primers, five primers (OPA 20, OPB 5, OPB 6, OPB 8, and OPC 19) producing polymorphic bands in RAPD analysis were selected. Different-shaped samples of sapodilla randomly collected from Bantul, Gunungkidul, Kulonprogo, Sleman and Yogyakarta city were used in this research. The analysis of molecular data was performed by using NTSys pc-2.02 and GenAlex 6.1 program. The clustering indicated that the accessions tend to group by their locations. However, there was no corelation between DNA marker and the fruit shape. The first group comprised samples from Yogyakarta city (Keraton Yogyakarta), Bantul and Kulonprogo while the second group consisted of samples from Sleman and Gunungkidul with genetic similarity of 53% and 37% respectively. The genetic diversity was high (PLP = 98.85% of 87 loci and H=0.280±0.14) comprising the genetic diversity within population (53%) which was greater than genetic diversity between populations (47%). Having the highest genetic diversity, Gunungkidul should be potential for conservation and selection of sapodilla in DIY.

Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand

“…The present study is first reported to have shown significant genetic polymorphism amongst various Manilkara accessions using SCoT markers (Heaton et al 1999;Meghala et al 2005;Thompson et al 2015;Martínez-Natarén et al 2017;Sari et al 2018). This examination, a set of 30 SCoT primers were preliminary verified in MZ01 sample from Sukhothai province to examine genetic polymorphisms, out of total, 30 SCoT primers produced unambiguous and reproducible banding profile with 120-1200 bp product size but only three SCoT primers (SCoT12, SCoT20, SCoT29) failed to amplify the Manilkara genomic DNA (Figure 2).…”

Review Artikel: Metode Ekstraksi DNA Genom untuk Tanaman Tinggi Kandungan Polisakarida dan Metabolit Sekunder

“…DNA yang berkualitas tinggi hasil ekstraksi harus dipenuhi dalam analisis molekuler. Berbagai teknik analisis dalam pemuliaan tanaman seperti identifikasi genotipe, keragaman genetik (Aboul-maaty & Oraby, 2019;Sari et al, 2018) dan biologi molekuler yang berdasarkan pada hibridisasi molekuler atau Polymerase Chain Reaction (PCR) membutuhkan DNA dalam jumlah yang cukup dan kualitas yang baik (Restu, 2012) sehingga isolasi dan pemurnian DNA menjadi langkah penting yang turut diperhatikan. Oleh karena kandungan metabolit sekunder dalam sel tanaman berbeda-beda, maka setiap tanaman membutuhkan prosedur isolasi yang optimum agar diperoleh DNA genom yang sesuai dengan syarat kebutuhan dalam analisis molekuler.…”

Studi Karakterisasi dan Keragaman Sifat Kualitatif Tanaman Rukam (Flacourtia rukam Zoll. & Mor.)

“…Kemajuan program pemuliaan akan sangat ditentukan oleh materi genetik yang tersedia, dimana semakin luas keragaman materi genetik yang dilibatkan dalam program pemuliaan suatu jenis, semakin besar peluang untuk mendapatkan peningkatan perolehan genetik (genetic rain) dari sifat yang diinginkan. Sedangkan Sari et al (2018), menyebutkan keberhasilan program pemuliaan tidak dapat terlepas dari keragaman genetik plasma nutfah yang digunakan dan hubungan antara aksesi yang digunakan sebagai tetua.…”