This article presents the procedure of designing and testing the virtual prototype of the one-track vehicle using the Autodesk Inventor Professional software. A multi-stage process of preparing the virtual prototype and the results of selected simulation tests were presented. During the first stage of work, a digital geometric model of the vehicle was created. The second stage includes placing the model in the dynamic simulation environment and defining the testing conditions corresponding to the actual vehicle operation conditions. The next phase relates to the realization of virtual model tests in the Autodesk Inventor dynamic simulation environment. The model was subjected to tests using the multibody systems method (MBS) to determine the forces and displacements in selected structural nodes of the vehicle, in motion on uneven ground conditions. The obtained simulation results in the form of force values over time and displacements were analyzed and conclusions were formulated. The test results can be used to conduct a more detailed endurance analysis of individual construction elements, model optimization and impact on the driver’s body. It was stated that the used method allows for a more efficient process of creating a new product and more efficient evaluation of the impact of the conditions in which it will be used.