In this work, the dynamic signal analyzer (DSA) based on Labview+Arduino has been developed. The DSA is validated to acquire and analyze vibration of two degrees of freedom (2-DOF) structure as the test rig. Vibration signal generated by bump test method to the test rig. Vibrations of the test rig are measured by using micro-electro-mechanical system (MEMS)-based accelerometers. The measured vibrations are acquired and analyzed by using the DSA. In the DSA is used fast Fourier transform (FFT) method to obtain natural frequencies of the structure and half power point (HPP) method to estimate damping ratio of structure. From the testing was conducted can be concluded that the DSA capable to determine natural frequency and damping ratio of the 2-DOF structure. If the obtained results are compared to theoretically results, there are different results about less than 10%.