Study on Endophytic Fungi Associated with Moringa oleifera Lam. Collected from Lombok Island, West Nusa Tenggara

Ramadhani, Indriati; Rohadi, Hasnadiazahra; Yuliani, Yeni; Ilyas, Muhammad

doi:10.14203/ann.bogor.2020.v24.n2.66-73

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Carbungco et al [ 17 ] isolated 24 fungal species from the leaves of M. oleifera that belonged to genera Fusarium, Xylaria, Pestalotiopsis, Aspergillus, Nigrospora, Stachybotrys, Rhizoctonia, and Macrophomina . Similarly, a number of endophytes belongs to Alternaria, Cladosporium, Colletotrichum, Corynespora, Curvularia, Fusarium, Mucor, Ochrocladosporium, Phomopsis and Trametes have been isolated from the M. oleifera gathered from Lombok Island, West Nusa Tenggara [ 31 ]. In fact, most of the endophytes that belong to the above-mentioned genera are already known for the production of bioactive compounds.…”

Section: Resultsmentioning

confidence: 99%

Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Rehman

Khan

Hamayun

et al. 2022

BioMed Research International

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Plant species are known to harbor large number of endophytes, which stays in plant tissues as symbionts. These endophytes secrete large array of bioactive compounds that have potency against certain diseases with no side effects. We have collected leaf samples of the Moringa oleifera plant from the Pakistan Forest Institute, Khyber Pakhtunkhwa, Pakistan for the isolation of beneficial endophytes. The strains isolated from the leaves of M. oleifera were coded with MOL and tested for antimicrobial, antifungal, germicidal, phytotoxic, insecticidal, cytotoxic, and anti-inflammatory activities. The isolates, MOL1, MOL16, MOL19, and MOL21, possessed antibacterial activity against Staphylococcus aureus, whereas MOL7 inhibited 55% of the growth of Escherichia coli. MOL3 inhibited the growth of E. coli, S. aureus, and Pseudomonas aeruginosa. The strains, MOL1 and MOL7, showed antifungal activity against Candida albicans and Saccharomyces cerevisiae, while the strains, MOL11 and MOL17, showed activity against Verticillium chlamydosporium. The isolates, MOL3, MOL7, MOL9, MOL15, MOL17, MOL18, and MOL19, inhibited the growth of Lemna minor (duckweed) at 100 μg/ml. MOL2 exhibited strong activity in the brine shrimp assay, while MOL1, MOL2, MOL5, MOL6, MOL12, MOL17, MOL19, and MOL20 showed insecticidal, and MOL3 demonstrated larvicidal and antileishmanial activity. The isolated potent endophytes were identified as Aspergillus, Penicillium, Fusarium, Tricoderma, Rhizoctonia, Mucor, Alternaria, Pestalotiopsis, Acremonium, and Cladosporium through morphological and microscopic characteristics of the colonies.

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Section: Resultsmentioning

confidence: 99%

Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Rehman

Khan

Hamayun

et al. 2022

BioMed Research International

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Secondary metabolites from endophytic fungi of moringa oleifera: antimicrobial and antioxidant properties

F¹,

O²

2022

JMEN

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The exponential increase in antimicrobial resistance among several microbes, now more than ever, poses a tremendous challenge to the health system. This has created an urgency to look outside the “norm” for antimicrobial agents that would be useful in tackling the various disease-causing microorganisms. Endophytes are microorganisms that not only have a niche within the plant tissues but also produce bioactive compounds which could be a significant breakthrough in the pharmaceutical industry, especially with the manufacture of novel drugs having an impactful effect on these disease-causing microbes. The study examined the secondary metabolites produced by endophytic fungi associated with Moringa oleifera as well as the antioxidant and antimicrobial activities of the crude extracts of these secondary metabolites against microbes of medical importance. To achieve this, the fresh leaves of moringa (Moringa oleifera) were collected from Ngwo in Enugu’s north local government area, Enugu State. Isolation of endophytic fungi was done using the protocol described by.13 The fungus was cultured by placing agar blocks of actively growing pure culture (3 mm in diameter) in a 500 ml Erlenmeyer flask containing 100 g of rice medium. Incubation was done at room temperature for 3 weeks. Afterward, the fermentation was stopped by introducing 500 ml of ethyl acetate into the flask. Whatman No. 1 filter paper was used to separate the fermentation mixture. Cultures of the bacteria and fungi were maintained on nutrient and Sabouraud dextrose agar respectively at 4 ºC. After the extraction had been done, antimicrobial activity was determined by agar well diffusion assay by Kirby-Bauer’s method. The result of the DPPH antioxidant assay of the endophytic fungal extract revealed that at a concentration of 100 µg/ml, Two Endophytic fungi, MA and MB showed antioxidant activity with % Inhibition of 55.1 and 48.3% respectively. The result of HPLC analysis of the fungal endophytic fungal metabolites revealed the presence of active compounds in the extracts. It was concluded that Moringa oleifera leaves harbor endophytic fungi. These endophytes could be exploited in medicine as novel metabolites.

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Study on Endophytic Fungi Associated with Moringa oleifera Lam. Collected from Lombok Island, West Nusa Tenggara

Cited by 2 publications

References 19 publications

Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Secondary metabolites from endophytic fungi of moringa oleifera: antimicrobial and antioxidant properties

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