Study on enzymes of industrial interest in digestive viscera: Greater amberjack (Seriola dumerili)

Oliveira, Vagne de Melo; Nascimento, Thiago Pajeú; Assis, Caio Rodrigo Dias; Bezerra, Ranilson de Souza; Porto, Ana Lúcia Figueiredo

doi:10.12980/jclm.5.2017j6-300

Cited by 5 publications

(8 citation statements)

References 31 publications

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“…Although the activity of digestive enzymes of various fish species such as Sardinops sagax caerulea (Castillo-Yañez et al, 2004), Sardinella aurita (Khaled et al, 2011), Salaria basilisca (Ktari et al, 2012), Pangasianodon gigas (Vannabun et al, 2014), Raja Clavata (Lassoued et al, 2015), Liza aurata (Bkhairia et al, 2016), Scomberomorus guttatus (Rengasamy et al, 2016), Seriola dumerili (Oliveira et al, 2017), Prochilodus lineatus (Gomez et al, 2018), Sardinella longiceps (Ramkumar et al, 2018) and Bagre panamensis (Rios-Herrera et al, 2019) have been extensively reported, a small number of researchers have focused on the digestive enzymes of Argentine marine fish species. In this sense, only trypsin from the viscera and heads of M. hubbsi (Lamas et al, 2015) and Engraulis anchoita (Lamas et al, 2017) have been characterized.…”

Section: P Brasiliensis C Guatucupa and Brazilian Codling Urophycis Brasiliensismentioning

confidence: 99%

“…Although a fraction of this waste is used in the elaboration of fishmeal (Mo et al, 2018), almost all of it is not used and is directly discarded on open-air dumps or water sources (Pereira & Fernandez Gimenez, 2016). Fishery by-products and wastes extracted from inexpensive marine feedstock may have an even higher value than the starting material, therefore it is necessary to identify technoeconomic viability to recycle these wastes (Oliveira et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison

Friedman

Behrens

Pereira

et al. 2021

Journal of Fish Biology

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Fish processing generates plenty of waste that is directly discarded in open-air dumps and water sources, or treated in the same way as urban solid waste, causing serious pollution problems. The waste represents a significant source of high-value bioproducts with potential applications in different industrial processes such as the production of feed, fertilizers, biodiesel and biogas, detergent additives and cosmetics. The objective of this study was to characterize and compare specific activities under different pH values and temperature conditions of acid and alkaline proteinases and viscera yield from the following fish species: Argentine hake Merluccius hubbsi, Brazilian flathead Percophis brasiliensis, Brazilian codling Urophycis brasiliensis and Stripped weakfish Cynoscion guatucupa. Individuals were fished off the coast of Mar del Plata (Argentina) by a commercial fleet and the viscera were immediately extracted and kept on ice until use. Stomach proteinases from four species had the highest activity at pH 2, with stability in the range of pH 2-4. The optimum pH was 11.5 from intestinal enzymes of C. guatucupa, M. hubbsi and P. brasiliensis and 9.5 from intestinal enzymes of U. brasiliensis. Alkaline proteinases from all species were highly stable in the range of 7-11.5. The optimum temperature of stomach proteinases from the four species studied were 30 and 50 C, with stability at 10 and 30 C during 150 min. The optimum temperature of intestinal enzymes from the tested species were 50 C with high stability at 10 and 30 C during 150 min. Alkaline proteinase from all species and acid proteinases from C. guatucupa were inactive at 70 C after 150 min, while there was a residual activity lower than 5% at 80 C on preincubated stomach enzymes of M.hubbsi, P. brasiliensis and U. brasiliensis after 5, 10 and 20 min, respectively. Digestive proteinases recovered in this study could be appropriate for technological usage, reducing manufacturing costs, obtaining revenue from fishery wastes, and contributing to the reduction of environmental pollution.

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Section: P Brasiliensis C Guatucupa and Brazilian Codling Urophycis Brasiliensismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison

Friedman

Behrens

Pereira

et al. 2021

Journal of Fish Biology

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“…From digestive viscera of aquatic organisms, it is possible to extract various proteases (pepsin, trypsin, chymotrypsin and collagenases and/or enzymes with similar catalytic functions) (Oliveira et al, 2017a;Gurumallesh et al, 2019;Silva et al, 2019). Enzymes with collagenolytic properties of aquatic organisms have already been recovered and partially purified from digestive residues of Neotropical fish species (Silva et al, 2019;Oliveira et al, 2017b;Liu et al, 2019). The collagenolytic property (collagen cleavage) is quite required in industry, mainly biomedical and pharmaceutical, being applied in surgical procedures (Zhao et al, 2019), intracerebral therapies (Chen-Roetling et al, 2019), wound healing (Abood et al, 2018), extraction of collagen and production of bioactive peptides (Bhagwat and Dandge, 2018), fibrosis treatment (Villegas et al, 2018), and treatment of Dupuytren (Zhang et al, 2019), thus making these enzymes fall into the category of high commercial value proteases (Oliveira et al, 2017c).…”

Section: Introductionmentioning

confidence: 99%

Extraction of Collagenolytic Enzyme From Fish Viscera by Phase Partitioning (Peg/Citrate) and Its Potential for Industrial Application

Silva¹,

Lino²,

Cunha³

et al. 2021

Bol. Inst. Pesca

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Internal viscera fish are potential sources of protein biomolecules of biopharmaceutical interest. However, this residue is frequently discarded inappropriately. The possibility to obtain by products of higher added value is a reality. Inside this view attention must be given to processes for the recovery and extraction of target molecules. However, the high cost of processing these residues is one of the obstacles to their reuse; techniques that facilitate their handling and make the process cheaper are desirable, such as extraction in a two-phase aqueous system. Thus, the aim of this study was to extract collagenolytic enzymes from common snook (Centropomus undecimalis) using a two-phase aqueous system (polyethylene glycol/citrate), according to the 24 factorial design, using as variables: molar mass of PEG (MPEG), PEG concentration (CPEG), citrate concentration (CCIT), pH, still, considering purification factor (FP), partition coefficient (K), and yield (Y). The collagenolytic activity of the crude extract was 102.41 U mg-1, after partitioning, was purified 3.91 times (MPEG: 8000; CPEG: 20.0%; CCIT: 20.0% and pH 6.0). Inhibition (U mg-1) was observed in benzamidine (22.51), TLCK (21.05), TPCK (21.29), and PMSF (23.05), signaling to be a serine-protease. The results showed the advantage of this semipurification technique as concerns to the low cost of extraction and purification, adding value to the fishing source material and allocating the residues from its processing to the industry.

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“…Although a fraction of this waste is used to the elaboration of shmeal (Mo et al 2018), large amounts of this generated solid waste is not used and is directly discarded on open-air dumps or water sources (Pereira and Fernández-Gimenez 2016). Fishery by-products and wastes extracted from inexpensive marine feedstock may have even higher in value than the starting material, so, it is necessary to identify techno-economic viability for recycling of these wastes (Oliveira et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Some of their distinctive features include greater stability over a wide range of pH, higher catalytic e ciency, and thermostability at low temperatures (Aspevik et al 2017). These characteristics have become suitable for different applications in many food-processing operations (Oliveira et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Digestive Proteases From Fish Processing Wastes: Their Partial Characterization and Comparison

Friedman¹,

Behrens²,

Pereira³

et al. 2020

Preprint

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Fish processing generates a lot of wastes which are discarded resulting in environmental problems. However, this material represents a significant source of high-value bioproducts with potential biotechnological applications. The objective of this study was to characterize and to compare specific activities of acid and alkaline proteases recovered from the viscera of Merluccius hubbsi (Mh), Percophis brasiliensis (Pb), Urophyis brasiliensis (Ub), and Cynoscion guatucupa (Cg) under different pH and temperature conditions. Stomach proteinases from four species had a higher activity at pH 2, with stability in the range of pH 2-4. Optimum pH from intestinal enzymes of Cg was 11.5, while for the crude extract of Mh, Pb, and Ub catalytic activity was registered over a wide pH range range from 7 to 11.5. Stomach proteinases from four studied species had a higher activity at 30 °C and 50 °C, with stability at 10 °C and 30 °C. Optimum temperature from intestinal enzymes of the four tested species was 50 °C with high stability at 10 °C and 30 °C. Alkaline proteinase from all species and acid proteinases from Cg was inactivated at 70ºC, while stomach enzymes of Mh, Pb, and Ub had a residual activity lower than 5% at 80 °C after 5, 10 y 20 minutes of pre-incubation, respectively. Digestive proteinases recovered in this study could be used as biocatalysts in industrial processes, reducing costs, adding value to the fishery waste, and contributing to the reduction of environmental pollution.

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Study on enzymes of industrial interest in digestive viscera: Greater amberjack (Seriola dumerili)

Cited by 5 publications

References 31 publications

Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison

Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison

Extraction of Collagenolytic Enzyme From Fish Viscera by Phase Partitioning (Peg/Citrate) and Its Potential for Industrial Application

Digestive Proteases From Fish Processing Wastes: Their Partial Characterization and Comparison

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