Study on Estimation and Antioxidant activity of Gloriosa superba L. Whole Plant Extract

Jebamalar, J. Angelin; Sivakumar, T.

doi:10.26438/ijsrbs/v6i3.5055

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…DPPH radical scavenging activity was determined by the standard protocol (15). 2 ml of DPPH methanol solution was then incorporated to the different sample solution (1mg/ml) at different volumes (20,40,60,80 and 100 µl).…”

Section: Assessment Of Free Radicals Scavenging Activity (Dpph)mentioning

confidence: 99%

In vitro antioxidant potential and antimicrobial activity of leaves and stem extracts of Anogeissus pendula Edgew.

2021

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Anogeissus pendula Edgew. is commonly used in the conventional Indian medicinal system and is reported to contain phenolic compounds which have antioxidant and antimicrobial potential. The goal of our study is to look at the antioxidant function and antibacterial activity of A. pendula leaf and stem extracts. The total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) were determined using a spectrophotometric technique (TTC). In vitro techniques such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide radical scavenging tests (H2O2) and Ferric reducing antioxidant power (FRAP) assay were used in the study. The disc diffusion technique was used to assess antibacterial activity and the minimum inhibitory concentration (MIC) was investigated against four bacterial strains. The TTC of leaf and stem methanol extract was considerably higher which ranged from 15.07 ± 0.506 to 38.77 ± 1.253 mg gallic acid equivalent (GAE) /g in leaves and 19.83 ± 0.084 to 28.56 ± 0.437 mg GAE/g in the stem. The content of flavonoid in the leaf and stem methanol extract varied from 12.53 ± 0.603 to 37.28 ± 0.466 mg rutin equivalent (RE) /g in leaves and 10.01 ± 0.177 to 37.28 ± 0.466 mg RE/g in stems. Hydroalcoholic extract of leaf and stem showed the highest tannin content and ranged from 23.73 ± 0.091 to 34.08 ± 0.261 mg tannic acid equivalent (TAE) /g. In order of efficacy (IC50) of the plant extracts, the effective inhibitor was the methanol extract of leaf and stem in the DPPH and H2O2 assay. FRAP value was higher in the hydroalcoholic extract of both leaf and stem. Antimicrobial activity tests revealed that all extracts limit the development of diverse microbial strains such as Escherichia coli, Bacillus subtilis, Pseudomonas putida and Streptococcus aureus with a mean zone of inhibition ranging from 0 to 15.67 mm. The MIC of A. pendula leaf and stem solvent extracts against bacterial strains ranged from 0.195 to 50 mg/ml. The findings revealed that A. pendula has a variety of phytochemicals with substantial antioxidant and antibacterial properties, confirming its usage in traditional medicine.

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Section: Assessment Of Free Radicals Scavenging Activity (Dpph)mentioning

confidence: 99%

In vitro antioxidant potential and antimicrobial activity of leaves and stem extracts of Anogeissus pendula Edgew.

2021

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“…Secondary metabolites or phytochemicals from plants have excellent pharmacological functions such as antioxidant, antimicrobial, anti-hypoglycemic and anticancer drugs. These bioactive compounds protect cells from the damage caused by unstable molecules called free radicals (Sivakumar and Panneerselvam, 2011;Anburaj et al, 2012;Sivakumar and Gajalakshmi, 2013;Sivakumar and Gajalakshmi, 2104;Senthilkumar and Sivakumar, 2014;Sivakumar et al, 2015;Senthil Kumar et al, 2016;Senthil Kumar et al, 2017;Harini and Nithyalakshmi, 2017;Jothi et al, 2019a;Jothi et al, 2019b;Sivakumar, 2019;Angelin et al, 2019). There is growing interests in preserving foods containing natural antimicrobial compounds, specifically extracted from plants.…”

Section: A Review On Some Folk Medicinal Plants and Their Common Usesmentioning

confidence: 99%

“…Whole plant (leaf, flower, seed, root) extract of G. superba otherwise called as flame lily, Calchicaceae containing colchicine and gloriosine are used externally and small quantity internally to Skin disease, Spines, Omitting, Indigestion, stomach-ache, Snake-bite and scorpion sting and antioxidant, antimicrobial activity (Jothi et al, 2019a;Jothi et al, 2019b).…”

Section: Gloriosa Supera Lmentioning

confidence: 99%

A Review on Some Folk Medicinal Plants and Their Common Uses

Sivakumar¹,

Balasubramanian²

2020

Research Biotica

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Current study aimed to Botanical features, applications in traditional medicinal plants, used to treat and prevent many types of diseases and systematically review publications indicating the viability of traditional medicinal plants. The leaves, fruits, seeds and bark of medicinal plants are used in traditional medicine to treat disorders affecting many systems, including blood circulation, the immune system, the heart, and digestive, respiratory, genetic, musculoskeletal, and conjunctive systems. Some herbal plants are used as pleasant spices, flavors, dyes, and preservatives food etc. Virtually every part of the plant has its own medicinal properties. Various types of phytochemicals found in medicinal plants play a key role in many types of diseases and are also used to produce medicines. The plant has various functions such as antimicrobial, antioxidant, anti-inflammatory, ant-diabetic, antibiotic, hemolytic properties that are widely used by indigenous peoples around the world. The folk medicinal plant uses of 12 herbal species belonging to various families are reported in this review article.

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Non-Alkaloidal Compounds from Tubers of Gloriosa superba and their In vitro and In silico Antibacterial and Antioxidant Activities

Dekebo

Melaku

Kiros

et al. 2023

CBC

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Background: The tubers of Gloriosa superba L. (Colchicaceae) are previously well known for their alkaloidal constituents. The present work aimed to investigate the non-alkaloidal constituents and evaluate their in vitro and in silico antibacterial and antioxidant activities from the tuber. background: The tubers part of Gloriosa superba L. (Colchicaceae) is previously well known by its alkaloidal constituents. The present work aimed to investigate the non-alkaloidal constituents and evaluate their in vitro and in silico antibacterial and antioxidant activities from the tuber part. Methods: The chloroform: methanol (1:1) tuber extract was fractionated over silica gel column chromatography, and isolated compounds were characterized by NMR spectroscopy. Various extracts and isolated compounds were evaluated for their antibacterial and antioxidant activities against certain pathogenic strains and oxidants. The in silico drug-likeness properties of isolated compounds were also studied against 6F86 antibacterial and 1HD2 antioxidant protein models. Results: Three non-alkaloidal compounds, β-sitosterol (1), 3-(cyclopenta-2,4-dienyloxy) β-sitosterol (2) and 1,2-n-dipropyl phthalate (3), were reported herein. The n-hexane and chloroform extracts displayed better antibacterial activity against E. coli (9.83 ± 0.28 mm) and P. aeruginosa (10.65 ± 0.79 mm), and S. aureus (10.33 ± 0.3 mm), respectively, at 100,000 µg/mL concentration. Compound 3 established a better activity against all bacterial strains (9.78 ± 0.63-11.07 ± 0.09 mm) at 1000 µg/mL. All the extracts exhibited a DPPH free radical scavenging activity comparable to ascorbic acid, whereas isolated compounds were found with negligible DPPH scavenging activity and weak ferric ion reduction power up to 500 µg/mL dose. The docking study revealed that all the compounds fulfilled Lipinski’s rule of five by contravening no more than one rule with strong binding affinity shown by compound 1 (-8.2 kcal/mol) and compound 3 (-4.5 kcal/mol) to the 6F86 bacterial and 1HD2 antioxidant protein models, respectively, which are comparable to the ciprofloxacin (-7.2 kcal/mol) and ascorbic acid (-4.5 kcal/mol) drugs. All the compounds also did not show any cytotoxicity properties. Conclusion: The promising antioxidant activity result of the various tubers extracts may highlight the potential use of Gloriosa superba as a source of foods by conducting further phytochemical investigation and additional bioassay evaluation, including the cytotoxicity effect of the whole part of the plant. result: Three non-alkaloidal compounds, β-sitosterol (1), 3-(cyclopenta-2,4-dienyloxy) β-sitosterol (2) and 1,2-n-dipropyl phthalate (3), were reported herein. The n-hexane and chloroform extracts displayed better antibacterial activity against E. coli (9.83 ± 0.28 mm) and P. aeruginosa (10.65 ± 0.79 mm), and S. aureus (10.33 ± 0.3 mm), respectively, at 100,000 µg/mL concentration. Compound 3 established a better activity against all bacterial strains (9.78 ± 0.63-11.07 ± 0.09 mm) at 1000 µg/mL. All the extracts exhibited a DPPH free radical scavenging activity comparable to ascorbic acid whereas isolated compounds were found with negligible DPPH scavenging activity and weak ferric ion reduction power up to 500 µg/mL dose. The docking study revealed that all the compounds fulfilled the Lipinski’s rule of five by contravening no more than one rule and did not show any cytotoxicity property. conclusion: Further phytochemical investigation may lead to isolation of additional non-alkaloidal bioactive constituents.

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Study on Estimation and Antioxidant activity of Gloriosa superba L. Whole Plant Extract

Cited by 4 publications

References 14 publications

In vitro antioxidant potential and antimicrobial activity of leaves and stem extracts of Anogeissus pendula Edgew.

In vitro antioxidant potential and antimicrobial activity of leaves and stem extracts of Anogeissus pendula Edgew.

A Review on Some Folk Medicinal Plants and Their Common Uses

Non-Alkaloidal Compounds from Tubers of Gloriosa superba and their In vitro and In silico Antibacterial and Antioxidant Activities

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