Study on Extractiing of Heme from Tilapias Blood by Enzymatic Method

Abstract

To make sure the abundance of Tilapias blood resources in China can be utilized more scientificly and effectively, the extraction conditions of heme from Tilapias blood by enzymatic hydrolysis were studied in this paper. The optimal hydrolysis conditions of protamex have been fixed by the single factor and the orthogonal experiments: The optimum temperature of 40°C, optimum pH of 8.0, substrate concentration (S) was 6%, enzyme/substrate (E/S) was 8000U/g, hydrolysis time (T) was 2 hours. Followed by the heat treatment, acid precipitation and vacuum freeze drying could get the heme at last, which yield was 80.9%, purity was 28.2%. This technology is simple and practical, low production cost and suitable for industrialized production, which is not only reduce environmental pollution, but also increase the economic efficiency of enterprises effectively. This research could provide theoretical evidence for making the best use of Tilapias blood resources.