Study on hepatotoxicity of aqueous extracts of Polygonum multiflorum in rats after 28-day oral administration-analysis on correlation of cholestasis

T, Wang; Wang, J; Jiang, Zhilan; Zhou, Zhong-Bo; Y, Li; Zhang, L

doi:10.4268/cjcmm20121021

Cited by 9 publications

(3 citation statements)

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“…Synthesis, metabolism, clearance and intestinal absorption of BAs has been reported to enrolled in hepatic diseases (Bobeldijk et al, 2008; Alnouti, 2009), and total serum bile acid has been suggested to be sensitive than some traditional assays for liver disease (Bouchier and Pennington, 1978). Our previous study has showed that DBIL and TBIL were more sensitive than ALT, AST, and ALP to liver injury induced by HSW (Tu et al, 2015), and aqueous extracts of HSW with high dose has reported to decrease the content of TBIL in serum (Wang et al, 2012). Therefore, detection of BAs seems to be an advisable method to detect toxicity of HSW.…”

Section: Discussionmentioning

confidence: 99%

“…Second, we need to further extend the coverage of metabolites by the untargeted metabolomic platform to assess if more sensitive DILI biomarkers can be discovered. Third, the dose of HSW used this study referred to HSW used in the assay of subacute toxicity (Wu et al, 2012), equivalent to 100 times of the upper dose of human stipulated in Chinese Pharmacopoeia (Wang et al, 2012), and histopathology of these rats has been studied to confirm that liver injury was appeared in raw HSW and chlorpromazine group, however the condition was similar but not equal to clinical condition. For the idiosyncratic hepatotoxins, more doses and time points at a clinical stage need to be detected to establish the clinical relevance to humans.…”

Section: Discussionmentioning

confidence: 99%

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Screening for biomarkers of liver injury induced by Polygonum multiflorum: a targeted metabolomic study

Qin

et al. 2015

Front. Pharmacol.

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Heshouwu (HSW), the dry roots of Polygonum multiflorum, a classical traditional Chinese medicine is used as a tonic for a wide range of conditions, particularly those associated with aging. However, it tends to be taken overdose or long term in these years, which has resulted in liver damage reported in many countries. In this study, the indicative roles of nine bile acids (BAs) were evaluated to offer potential biomarkers for HSW induced liver injury. Nine BAs including cholic acid (CA) and chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), ursodeoxycholic acid (UDCA), and hyodeoxycholic acid (HDCA) in rat bile and serum were detected by a developed LC-MS method after 42 days treatment. Partial least square-discriminate analysis (PLS-DA) was applied to evaluate the indicative roles of the nine BAs, and metabolism of the nine BAs was summarized. Significant change was observed for the concentrations of nine BAs in treatment groups compared with normal control; In the PLS-DA plots of nine BAs in bile, normal control and raw HSW groups were separately clustered and could be clearly distinguished, GDCA was selected as the distinguished components for raw HSW overdose treatment group. In the PLS-DA plots of nine BAs in serum, the normal control and raw HSW overdose treatment group were separately clustered and could be clearly distinguished, and HDCA was selected as the distinguished components for raw HSW overdose treatment group. The results indicated the perturbation of nine BAs was associated with HSW induced liver injury; GDCA in bile, as well as HDCA in serum could be selected as potential biomarkers for HSW induced liver injury; it also laid the foundation for the further search on the mechanisms of liver injury induced by HSW.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Screening for biomarkers of liver injury induced by Polygonum multiflorum: a targeted metabolomic study

Qin

et al. 2015

Front. Pharmacol.

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“…In addition, the oral administration of the acetone extract of raw PMR is much more toxic than that of the acetone extract of processed PMR [31]. In addition to these reports, many studies have described the hepatotoxicity of PMR in China [32–34] and Korea [35–38]. Most of these reports were related to individuals who were taking PMR without a doctor's prescription or for a long time or those who overdosed.…”

Section: Toxicologymentioning

confidence: 99%

Discrimination and Proper Use of Polygoni Multiflori Radix, Cynanchi Wilfordii Radix, and Cynanchi Auriculati Radix in Korea: A Descriptive Review

Lee

2015

Evidence-Based Complementary and Alternative Medicine

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Polygoni Multiflori Radix (PMR), Cynanchi Wilfordii Radix (CWR), and Cynanchi Auriculati Radix (CAR) are very popular herbal medicines in Traditional Korean Medicine, Traditional Chinese Medicine, and Kampo Medicine. However, the plant origins, efficacies, and traditional uses of these herbal medicines differ. In Korea, PMR is called Ha Su O (He Shou Wu in China), and CWR is called Baek Ha Su O or Baek Su O (Bai Shou Wu in China). Baek Su O refers to CWR in Korea and CAR in China. CAR has not been used as a traditional herbal medicine, and it cannot be legally used as a food or food ingredient in Korea. However, CAR is cultivated in Korea and imported from China. Because the morphology of CWR and CAR is very similar, they are often confused and misused in Korea. This review discusses the reasons for the confusion and misuse of these substances in Korea and provides the exact plant origins, efficacies, uses, components, and toxicities of PMR, CWR, and CAR so that they can be correctly understood and used.

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2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside exacerbates acetaminophen-induced hepatotoxicity by inducing hepatic expression of CYP2E1, CYP3A4 and CYP1A2

Liu

Shi

et al. 2017

Sci Rep

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Hepatotoxicity induced by medicinal herb Polygonum multiflorum Thunb. attracts wide attention in the world recently. 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is a main active compound in Polygonum multiflorum Thunb. This study aims to observe TSG-provided the aggravation on acetaminophen (APAP)-induced hepatotoxicity in mice by inducing hepatic expression of cytochrome P450 (CYP450) enzymes. Serum alanine/aspartate aminotransferase (ALT/AST) analysis and liver histological evaluation showed that TSG (200, 400, 800 mg/kg) exacerbated the hepatotoxicity induced by sub-toxic dose of APAP (200 mg/kg) in mice, but TSG alone had no hepatotoxicity. TSG aggravated hepatic reduced glutathione (GSH) depletion and APAP-cysteine adducts (APAP-CYS) formation induced by APAP in mice. TSG increased the expression of CYP2E1, CYP3A4 and CYP1A2 both in mice and in human normal liver L-02 hepatocytes. TSG also enhanced liver catalytic activity of CYP2E1, CYP3A4 and CYP1A2 in mice. TSG induced the nuclear translocation of aryl hydrocarbon receptor (AHR) and pregnane X receptor (PXR), and TSG-provided the aggravation on APAP-induced hepatotoxicity in mice was reversed by PXR or AHR inhibitors. In summary, our results demonstrate that TSG enhances hepatic expression of CYP3A4, CYP2E1 and CYP1A2, and thus exacerbates the hepatotoxicity induced by APAP in mice. PXR and AHR both play some important roles in this process.

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Study on hepatotoxicity of aqueous extracts of Polygonum multiflorum in rats after 28-day oral administration-analysis on correlation of cholestasis

Cited by 9 publications

References 0 publications

Screening for biomarkers of liver injury induced by Polygonum multiflorum: a targeted metabolomic study

Screening for biomarkers of liver injury induced by Polygonum multiflorum: a targeted metabolomic study

Discrimination and Proper Use of Polygoni Multiflori Radix, Cynanchi Wilfordii Radix, and Cynanchi Auriculati Radix in Korea: A Descriptive Review

2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside exacerbates acetaminophen-induced hepatotoxicity by inducing hepatic expression of CYP2E1, CYP3A4 and CYP1A2

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