STUDY ON Hibiscus tiliaceus LEAVES AS ANTIBACTERIAL AND ANTIOXIDANT AGENTS

Abstract

This study aimed to evaluate antioxidant and antibacterial activities of methanol, hexane, dichloromethane, ethyl acetate, and water crude sample fractions of H. tiliaceus leaves. Various type of samples play an important role to determining which compound that will give the positive results in antioxidant test (inhibiting free radical scavenging activity) which they probably have potency for preventing the cancer disease. Furthermore, those types of samples were also tested to the antibacterial test as their possibilities to be created as new antibiotics in the future. This research also focused on what compound groups that actually present in H. tiliaceus leaves by using the phytochemistry test. Antioxidant potency of H. tiliaceus leaves extracts for the radical scavenging activity for quantitative assay was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antibacterial potency was measured by the Muller Hinton Agar (MHA) by applied the sample chemical crude in the plate. The result showed that all chemical crude of H. tiliaceus leaves has high antioxidant property as methanol crude (MC) 308.416%; hexane crude (HC) 232.837%; dichloromethane crude (DC) 150.837%; ethyl acetate crude (EC) 73.623% and water crude (WC) 71.777% with respected to the readings of 100% from quercetin (Q) as a positive control. From antibacterial result, entire samples had shown the positive results towards both gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). The ranges of the inhibition zone were both at 7-10 mm and 10-15 mm. The phytochemistry test determine that the samples actually contained a lot of compounds which were proteins, carbohydrates, phenols/tannins, flavonoid, terpenoids, glycosides and also steroid. It is clearly indicated that H. tiliaceus leaves have potential to be used against the antioxidant and also as new antibacterial agents

Kadar Flavonoid dan Fenolik Ekstrak Daun Waru (Hibiscus tiliaceus) Serta Aktivitasnya Sebagai Antioksidan

“…Flavonoid yang tergolong dalam senyawa fenolik, dikenal sebagai komponen bioaktif pada makanan, mengandung sumber antioksidan eksogen yang bersifat alami. Kandungan yang dimiliki flavonoid memiliki efek farmakologis sebagai bahan baku pembuatan obat-obat tradisional karena memiliki beberapa khasiat baik sebagai antifungi, antihistamin, antihipertensi, antibakteri, antivirus (Emelda, 2020), anti inflamasi, dan antikanker (Lumbessy, dkk., 2013;Samsudin et al, 2019) Tanaman waru (Hibiscus tiliaceus) merupakan salah satu Genus dalam kelompok Familia Malvaceae yang memiliki kurang lebih 2.000 spesies dan tersebar di kawasan tropis maupun sub tropis. Kandungan utama dalam waru yaitu senyawa fenolik atau polifenolik yang juga sangat bermanfaat sebagai antioksidan yang berguna melawan radikal bebas (Kumar dkk., 2008).…”

“…Ekstrak etanol daun waru dilaporkan mengandung komponen fenolik, yang memiliki aktivitas antioksidan. Ekstrak metanol daun waru (Hibiscus tiliaceus) juga telah diteliti memiliki aktivitas antioksidan (Hossain et al, 2015;Samsudin et al, 2019;Andriani et al, 2020).…”

“…Ekstrak kasar masih mengandung senyawa lain yang bukan merupakan senyawa antioksidan yang larut dalam proses ekstraksi (Badriyah, Achmadi and Nuswantara, 2017). Akan tetapi, hasil ini memberikan hasil yang sama dengan penelitian sebelumnya bahwa ekstrak metanol daun waru menunjukkan nilai aktivitas antioksidan yang paling tinggi yaitu 120,941 µg/mL dibandingkan pelarut lainnya yang sama-sama bersifat polar (air) yaitu 218,876 µg/mL, semi polar (etil asetat dan diklorometana) yaitu 363,248 µg/mL dan 770,835 µg/mL, bahkan pelarut non polar (heksana) dengan nilai antioksidan yaitu 320,575 µg/mL (Samsudin et al, 2019).…”

Antibacterial and Antioxidant Activity of Endophytic Bacteria Isolated from Hibiscus tilaceus Leaves

“…Besides that, in the waru tree, there are saponins in the leaves and roots. Samsudin et al, 2019 reported that waru leaf extract is antibacterial and antioxidant. Ramposhad et al, 2012 also reported that waru leaf extract could inhibit the growth of 3 strains: Escherichia coli, Salmonella parathyphi, and Staphylococcus aureus.…”

AKTIVITAS SITOTOKSIK DAN PENGHAMBATAN MIGRASI SEL KANKER 4T1 DARI EKSTRAK DAUN WARU (Hibiscus tiliaceus Linn.)

“…Ekstrak daun waru memiliki efek sitotoksik pada sel kanker payudara jenis MCF-7 (Andriani et al, 2020). Kandungan metabolit sekunder ekstrak daun waru yaitu polifenol, flavonoid, terpenoid, steroid dan glikosida (Samsudin et al, 2019). Penelitian ini dilakukan untuk mengetahui aktivitas sitotoksik dan penghambatan migrasi dari ekstrak daun waru terhadap sel kanker 4T1.…”