Study on<i>RIZ1</i>gene promoter methylation status in human esophageal squamous cell carcinoma

Dong, Siming; Zhang, Peng; Liu, Yimei; Cui, Yuantao; Wang, Shuo; Liang, Shao-Jie; He, Zhun; Sun, Pei; Wang, Yuan-Guo

doi:10.3748/wjg.v18.i6.576

Cited by 17 publications

(24 citation statements)

References 17 publications

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“…Lal et al (27) demonstrated that RIZ1 expression is lost in thyroid tumor cell lines and is also significantly reduced in thyroid carcinomas, when compared with normal thyroid tissues (p<0.0001) and benign tumors (p=0.0003). Wen dong et al (28) found that RIZ1 gene is silenced in human esophageal squamous cell carcinoma and it is concentration is down regulated. Finaly Khaenam et al (29) found that (RIZ1) inactivated by promoter hypermethylation in patients with liver fluke-related cholangiocarcinoma (CCA).…”

Section: Discussion:-mentioning

confidence: 99%

“…It is the 2 nd most common malignancy related mortality worldwide with 745,000 deaths per year (2). Its incidence is continuously increasing throughout the world (3)(4)(5). In Egypt, between 1993 and 2002, there was an almost two fold increase in HCC among patients with chronic liver disease (6).…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

“…Epigenetic events are considered key mechanisms in the regulation of gene activity, and abnormal expression of a large number of tumor suppressor genes and cancer-associated genes has been observed in a wide range of human cancer (28). DNA methylation has been primarily studied in the context of gene transcription and aberrant gene silencing, although it has been implicated in the silencing of transposable elements (29).…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

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Diagnostic Value of Riz1 Gene Expression as a Potential Marker for Patients With Hepatocellular Carcinoma.

Metwaly¹,

Abassy²,

Zakareya³

et al. 2016

IJAR

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Section: Discussion:-mentioning

confidence: 99%

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic Value of Riz1 Gene Expression as a Potential Marker for Patients With Hepatocellular Carcinoma.

Metwaly¹,

Abassy²,

Zakareya³

et al. 2016

IJAR

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“…The results suggested that RIZ1 was decreased (t = -57.738, p = 0.000) while RIZ2 was uniformly present in ESCC carcinoma (t = -1.180, p = 0.249) compared with the corresponding normal tissue specimens. Promoter region high methylation status serves as an important mechanism in RIZ1 down regulation or deletion [8,22]. In this study RIZ1 promoter region aberrant methylation was detected by MSP in ESCC cell line EC109.…”

Section: Discussionmentioning

confidence: 99%

“…RIZ1 was proved to be a tumor suppressor gene in ESCC in our previous studies, whereby RIZ1 was lowly expressed or deleted in ESCC tumor tissues and cell lines. Promoter region aberrant methylation serves as an important role [8,22]. In this paper Real-time PCR was used to examine both the expression levels of RIZ1 and RIZ1 + RIZ2 mRNA in ESCC carcinoma and corresponding normal tissue specimens.…”

Section: Discussionmentioning

confidence: 99%

The unusual yin-yang fashion of RIZ1/RIZ2 contributes to the progression of esophageal squamous cell carcinoma

et al. 2016

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Retinoblastoma protein-interacting zinc finger gene RIZ encodes two different protein products, RIZ1 and RIZ2. Observations suggest that RIZ1 is a tumor suppressor, while RIZ2 acts as a negative regulator of RIZ1 and may play a positive role in oncogenesis. The imbalance amount of RIZ1 and RIZ2 may be involved in cancer development. In this study we detected the expression levels of RIZ1 and RIZ2 mRNA in human esophageal squamous cell carcinoma (ESCC) tissue specimens, reexpressed RIZ1 in the human ESCC cell line EC109 in which RIZ1 mRNA level was not detected, examined the changes of RIZ1 and RIZ2 mRNA expression, investigated the changes of proliferation, and apoptosis of the cells. We found that RIZ1 mRNA expression is commonly decreased or at undetectable level in human esophageal squamous cancer tissue specimens compared to the normal tissue specimens, while RIZ2 is usually expressed. With the forced expression of RIZ1, RIZ2 mRNA expression did not change, The ESCC cell proliferation was inhibited and apoptosis was induced. This unusual yinyang fashion of RIZ1/RIZ2 may contribute to the progression of ESCC.

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Methylation Status of the RIZ1 Gene Promoter in Human Glioma Tissues and Cell Lines

Zhang

Wang

et al. 2016

Cell Mol Neurobiol

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Retinoblastoma protein-interacting zinc-finger gene 1 (RIZ1), a strong tumor suppressor, is silenced in many human cancers. Our previous studies showed that RIZ1 expression was negatively correlated with the grade of glioma and was a key predictor of patient survival. Therefore, RIZ1 could be a potential tumor suppressor during glioma pathogenesis, although the mechanism underlying RIZ1 gene inactivation in gliomas is unknown. We investigated the methylation status of the RIZ1 promoter in human glioma tissues and four glioblastoma (GBM) cell lines, and verified the effect of the methyltransferase inhibitor 5-aza-2-deoxycytidine (5-aza-CdR) on RIZ1 transcription and cell proliferation. Methylation-specific PCR (MSP) was performed to determine RIZ1 promoter methylation in human glioma specimens. The correlation between RIZ1 hypermethylation in tumors and clinicopathological features also was analyzed. 5-Aza-CdR treatment was used to reactivate gene expression silenced by hypermethylation in the U87 glioblastoma cell line, and real-time PCR was then used to measure RIZ1 expression. The ability of 5-aza-CdR to inhibit the proliferation of glioma cell lines whose RIZ1 promoters were hypermethylated was measured by bromodeoxyuridine (BrdU) incorporation. Among 51 human glioma specimens, RIZ1 promoter methylation was detected in 23 cases. Clinicopathological evaluation suggested that RIZ1 hypermethylation was negatively associated with tumor grade and patient age (P < 0.05). Hypermethylation of the RIZ1 promoter was detected in the U87 and U251 cell lines. RIZ1 mRNA expression in U87 cells was upregulated after treatment with 5-aza-Cdr, which correlated with inhibition of cell proliferation in a time- and concentration-dependent manner. Promoter hypermethylation may play an important role in the epigenetic silencing of RIZ1 expression in human glioma tissues and GBM cell lines.

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Study onRIZ1gene promoter methylation status in human esophageal squamous cell carcinoma

Abstract: Promoter methylation may play an important role in the epigenetic silencing of RIZ1 gene expression in human ESCC. RIZ1 is considered to be a potential tumor suppressor gene and may be a biological parameter for testing early stage human ESCC.

Cited by 17 publications

References 17 publications

Diagnostic Value of Riz1 Gene Expression as a Potential Marker for Patients With Hepatocellular Carcinoma.

Diagnostic Value of Riz1 Gene Expression as a Potential Marker for Patients With Hepatocellular Carcinoma.

The unusual yin-yang fashion of RIZ1/RIZ2 contributes to the progression of esophageal squamous cell carcinoma

Methylation Status of the RIZ1 Gene Promoter in Human Glioma Tissues and Cell Lines

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