“…The metabolic molecules associated with symbiotic bacteria living in deep-sea cold seeps are of high research value. − Benefiting from the development of deep-sea in situ detection technology, various spectral instruments have been successfully applied to the detection of deep-sea methane, carbonate, carbon dioxide and other molecules. − However, due to the low concentration of organic molecules in the deep-sea environment, the traditional detection technology is difficult to achieve the in situ detection of deep-sea organic molecules. , Surface-enhanced Raman scattering (SERS) technology became an effective means to solve this problem due to its advantages of high sensitivity, no need for sample pretreatment and fast real-time detection. − However, the high pressure, high salt, and complex fluid environment in the deep sea posed a serious challenge to the application of SERS technology in the deep sea. , Solid SERS substrate is the first choice for deep sea in situ detection because of its strong stability and pressure resistance. , The SERS substrates were successfully applied to cold seep vents in our previous work . Although Raman spectral information on various biological macromolecules was obtained, the defects of traditional solid SERS substrates applied in deep sea were also exposed.…”