Study on LOC426217 as a candidate gene for beak deformity in chicken

Bai, Hao; Sun, Yanyan; Zhu, Jing; Liu, Nian; Li, Dongli; Xue, Fuguang; Li, Yunlei; Chen, Jilan

doi:10.1186/s12863-016-0353-x

Cited by 9 publications

(13 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was estimated that 0.32% of all wild birds have crossed beaks [ 11 ]. In a recent study, prevalence ranging from 1 to 3% was documented for local Chinese chicken breeds [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Most of the identified genes belonged to the keratin family, as well as genes coding for proteins important in the biosynthesis of unsaturated fatty acids and the metabolism of glycerolipids. A follow-up study on the most promising gene LOC426217 found five variants that differed significantly between chickens with crossed beaks and normal controls [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Crossed beaks in a local Swiss chicken breed

Joller

Bertschinger

Kump³

et al. 2018

BMC Vet Res

View full text Add to dashboard Cite

BackgroundCrossed beaks have been reported to occur in Appenzeller Barthuhn, a local Swiss chicken breed. The assumed causes for this beak deformity which are also seen in other bird species including domestic chickens, range from environmental influences to genetic factors. The aim of this project was to characterize the prevalence, the phenotype, and the underlying genetics of crossed beaks in Appenzeller Barthuhn chickens.ResultsThe estimated prevalence of 7% crossed beaks in Appenzeller Barthuhn was significantly higher compared to two other local Swiss chicken breeds. A breeding trial showed significantly higher prevalence of offspring with deformed beaks from mating of affected parents compared to mating of non-affected parents.Examination of 77 Appenzeller Barthuhn chickens with crossed beaks showed a variable phenotype presentation. The deviation of the beak from the median plane through the head ranged from 1° to 61°. In more than 60% of the cases, the upper and lower beak were bent in the same direction, whereas the remaining cases showed different forms of crossed beaks. Computed tomographic scans and bone maceration of the head of two chickens with crossed beaks revealed that the maxilla and the mandibula were affected, while other parts of the skull appeared to be normal.The gene LOC426217, a member of the keratin family, was postulated as a candidate gene for beak deformity in domestic chickens. Sequencing of the coding region revealed two significantly associated synonymous variants for crossed beaks in Appenzeller Barthuhn chickens. A genome-wide association study and a comparative analysis of runs of homozygosity based on high-density SNP array genotyping data of 53 cases and 102 controls showed no evidence of association.ConclusionsThe findings suggest a hereditary cause of crossed beaks in Appenzeller Barthuhn chickens. However, the observed variation in the phenotype, together with the inconclusive molecular genetic results indicates the need for additional research to unravel the genetic architecture of this beak deformity.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1398-z) contains supplementary material, which is available to authorized users.

show abstract

“…It was estimated that 0.32% of all wild birds have crossed beaks [ 11 ]. In a recent study, prevalence ranging from 1 to 3% was documented for local Chinese chicken breeds [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Crossed beaks in a local Swiss chicken breed

Joller

Bertschinger

Kump³

et al. 2018

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…Cross-beaks are a disease resulting in economic loss for the poultry industry and comprise 1% to 3% in Chinese local chickens [2]. A beak consists of multiple facial prominences.…”

Section: Discussionmentioning

confidence: 99%

“…A cross-beak is a dysmorphology relating to deformed beaks and has been suggested as a threat to animal welfare breeding. There are 1%-3% of Chinese native chickens that have beak deformities, leading to great economic loss and animal welfare issues in the poultry industry [1][2][3]. Typically, cross-beaks refer to the misalignment of upper and lower beaks.…”

Section: Introductionmentioning

confidence: 99%

The Morphology of Cross-Beaks and BMP4 Gene Expression in Huiyang Bearded Chickens

Hong

Pang

Zhao

et al. 2019

Animals

View full text Add to dashboard Cite

Bird beaks are important for biological purposes such as food intake, removing parasites, and defining phenotypic attributes. Cross-beaks are a threat to poultry health and are harmful to productivity, wasting some units in the poultry industry. However, there is still limited research on subtypes of cross-beaks and the genetic basis of cross-beaks as well. Here, we described the subtypes of cross-beaks in terms of left or right and upper or lower jaw bones. We evaluated the impact of cross-beaks on craniofacial bones and figured out the relationship between bone morphogenetic protein 4 (BMP4) and the development of craniofacial bones in Huiyang bearded chickens. We identified five typical subtypes of cross-beaks by morphological assessment and X-ray scanning. We found that cross-beaks caused certain changes in the facial bone morphology, including changes to the length and width of the bone around the ocular area (p < 0.05). The relative expressions of BMP4 in lacrimal, mandible, premaxilla, frontal, and parietal bones were significantly higher in the severe cross-beak group, followed by that of the medium cross-beak group, weak cross-beak group, and control group (p < 0.05). Overall, we constructed a generally applicable method to classify cross-beaks in term of the angle. The skeleton around the ocular area was affected by the cross-beak. The expression levels of BMP4 in craniofacial bones may provide insight to potential role of BMP4 in the development of cross-beaks.

show abstract

“…unsaturated fatty acid biosynthesis and glycerolipid metabolism) that are associated with crossed beaks in chickens (Bai et al . , ).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide detection ofCNVs associated with beak deformity in chickens using high‐density 600KSNParrays

et al. 2018

View full text Add to dashboard Cite

Beak deformity (crossed beaks) is found in several indigenous chicken breeds including Beijing-You studied here. Birds with deformed beaks have reduced feed intake and poor production performance. Recently, copy number variation (CNV) has been examined in many species and is recognized as a source of genetic variation, especially for disease phenotypes. In this study, to unravel the genetic mechanisms underlying beak deformity, we performed genome-wide CNV detection using Affymetrix chicken high-density 600K data on 48 deformed-beak and 48 normal birds using penncnv. As a result, two and eight CNV regions (CNVRs) covering 0.32 and 2.45 Mb respectively on autosomes were identified in deformed-beak and normal birds respectively. Further RT-qPCR studies validated nine of the 10 CNVRs. The ratios of six CNVRs were significantly different between deformed-beak and normal birds (P < 0.01). Within these six regions, three and 21 known genes were identified in deformed-beak and normal birds respectively. Bioinformatics analysis showed that these genes were enriched in six GO terms and one KEGG pathway. Five candidate genes in the CNVRs were further validated using RT-qPCR. The expression of LRIG2 (leucine rich repeats and immunoglobulin like domains 2) was lower in birds with deformed beaks (P < 0.01). Therefore, the LRIG2 gene could be considered a key factor in view of its known functions and its potential roles in beak deformity. Overall, our results will be helpful for future investigations of the genomic structural variations underlying beak deformity in chickens.

show abstract

Study on LOC426217 as a candidate gene for beak deformity in chicken

Cited by 9 publications

References 37 publications

Crossed beaks in a local Swiss chicken breed

Crossed beaks in a local Swiss chicken breed

The Morphology of Cross-Beaks and BMP4 Gene Expression in Huiyang Bearded Chickens

Genome‐wide detection ofCNVs associated with beak deformity in chickens using high‐density 600KSNParrays

Contact Info

Product

Resources

About