Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris

Lee, Jong Seok; Kwon, Jeong Seok; Won, Dong Pil; Lee, Keun Eok; Shin, Wan Seon; Hong, Eock Kee

doi:10.1016/j.carbpol.2010.06.025

Cited by 59 publications

(15 citation statements)

References 34 publications

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“…The results of the monosaccharide‐composition analysis through GC are shown in Figure . Compared with some polysaccharides which have antioxidant and immunological activity (Kim, Park, Nam, Lee, & Lee, ; Lee et al, ; Liu et al, ), SGP I has a similar molecular mass and SGP II has a lower molecular mass. SGP I‐b comprised four monosaccharides, namely, fucose, mannose, glucose, and galactose at the molar ratios of 1.86:1.61:1:4.14.…”

Section: Resultsmentioning

confidence: 99%

Structural characterization, antioxidant activity, and immunological activity in vitro of polysaccharides from fruiting bodies of Suillus granulatus

Chen

Wang

2018

J Food Biochem

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Two polysaccharide fractions, SGP I-b and SGP II-b, were purified from the fruiting bodies of Suillus granulatus. The assayed relative molecular masses of SGP I-b and SGP II-b were approximately 17.31 and 10.16 kDa, respectively. SGP I-b consisted of fucose, mannose, glucose, and galactose, whereas SGP II-b consisted of rhamnose, fucose, mannose, glucose, and galactose. The structures of SGP I-b and SGP II-b were preliminarily analyzed through methylation analysis and nuclear magnetic resonance spectroscopy. The results of in vitro antioxidant assays revealed that SGP II-b demonstrated good scavenging ability for hydroxyl and DPPH radicals but poor reduction capacity and scavenging for superoxide anions. SGP I-b did not exhibit any significant antioxidant activities. SGP II-b could also significantly increase lymphocyte proliferation in vitro, however, SGP I-b had no significant effect. Practical applicationsSuillus granulatus is a wild, edible medicinal fungus distributed in Northeastern China. S. granulatus polysaccharides (SGP) already be prove have special bioactivities. In the current study, SGP were purified and characterized, and the antioxidant activities and immunomodulatory effects were also assayed. Our work will contribute to the utilization of SGP as natural antioxidants and/or immunity-enhancing agents in food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Structural characterization, antioxidant activity, and immunological activity in vitro of polysaccharides from fruiting bodies of Suillus granulatus

Chen

Wang

2018

J Food Biochem

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“…In this field, calcofluor has been found to be a useful and rapid chemofluorescent stain for detection of β configuration in polysaccharides. Calcofluor white, namely cellufluor, binds nonspecifically to β-linked polysaccharides and the sample fluoresces an intense green whereas α configurations do not exhibit fluorescence [38].…”

Section: Configuration Of the Glycosidic Bondmentioning

confidence: 99%

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

2012

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Abstract:Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide.

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“…Together with reported results, the antitumor effect mechanism of C. militaris polysaccharides could be immuno-enhancing activity. Polysaccharides exert their antitumor effects primarily by activating various immune system responses in the host, such as complement system activation (Dennert and Tucker, 1973), macrophage-dependent immune system responses Lee et al, 2010), and upregulation of interferon expression (Hamuro and Chihara, 1985).…”

Section: Discussionmentioning

confidence: 99%

Cordyceps militaris polysaccharide triggers apoptosis and G0/G1 cell arrest in cancer cells

Cheng

Wang

Jin

et al. 2015

Journal of Asia-Pacific Entomology

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Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris

Cited by 59 publications

References 34 publications

Structural characterization, antioxidant activity, and immunological activity in vitro of polysaccharides from fruiting bodies of Suillus granulatus

Structural characterization, antioxidant activity, and immunological activity in vitro of polysaccharides from fruiting bodies of Suillus granulatus

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

Cordyceps militaris polysaccharide triggers apoptosis and G0/G1 cell arrest in cancer cells

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