Study on Measurement of Reduction Current of Oxidant Mediator by Electrochemical Array Sensor Using Internal Photoelectric Effect

Kosugi, Keita; Uchida, Hidekazu

doi:10.1541/ieejsmas.139.400

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…This study aimed at a method of AChE inhibitor screening with the two-dimensional electrochemical sensor LAAS 10,11 developed in our laboratory. In the LAAS used in this study, multiple gold electrodes are arranged on the sensor surface, but only portions irradiated with an excitation laser are measured; thus, there is no need for individual electrode wiring, and parallel unlabeled measurements can be realized easily.…”

Section: Research Backgroundmentioning

confidence: 99%

Measurement of acetylcholinesterase using a two‐dimensional electrochemical sensor LAAS

Miyairi,

Hasegawa,

Uchida

2023

Elect Comm in Japan

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In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples. Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by AChE and releases electrons, making it possible to measure current values in accordance with the amount of substrate using LAAS. When KCl solution was used as the supporting electrolyte and an Ag/AgCl reference electrode as the counter electrode, a correlation between substrate concentration and reaction was confirmed in the range of 1 μM–10 mM. Malathion was then used as an inhibitor of AChE. Malathion phosphorylates and inactivates AChE, which prevents substrate hydrolysis and is expected to decrease the current value. Experimental results showed a decrease in sensor response correlated with inhibitor concentration.

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Section: Research Backgroundmentioning

confidence: 99%