Different methods were used to repair concrete cracks. Recently, microbial induced calcium carbonate precipitation is presented as a self-healing technique for concrete manufacture, which is proposed as an eco-friendly method. It is a bio-mineralization process. Microbial activities induced precipitation that can heal cracks. Effect of bio-mineralization in concrete by incorporation of ureolytic species such as Bacillus Megaterium is investigated. Fresh and hardened tests were carried out on concrete at various ages. "Bacillus Megaterium" (Bm) with a cell density of 2× CFU/mL was used when added to mixtures with two ratios of 0.5% and 0.25% of cement weight. Calcium lactate, Calcium acetate, and Calcium format were added as nutrition to bacteria by 0.25% and 0.125% of cement weight. Test results indicated that the inclusion of Bm in concrete enhanced properties. Bacteria addition and all nutritions used revealed varied decreases in the rate of water absorption and capillary permeability coefficient. Bm 0.5% with acetate nutrition concrete mix had a maximum increase in compressive strength at all ages. It had compressive strength value 152.99% of that of the compressive strength of the control concrete mixture at 28days. Bm 0.5% with lactate nutrition concrete mix had flexural strength value 162.74% of that of control concrete flexural strength at 28days. Enhancement of properties was due to calcite deposition on the bacteria cell surfaces within the pores. SEM imaging indicated the formation of calcite crystals in different shapes according to the added nutrients of bacterial concrete specimens.