Study on Melting Behavior of Iron Tailings Particles in the Blast Furnace Slag

A “click” chemistry constructed affinity system for 2-oxoglutaric acid receptors and binding proteins

“…2A, the hydrolysis of the ester groups in 7 to give the corres-ponding carboxylate functions was highlighted by the signal of the carbonyl group shifting from 1707 cm −1 (characteristic of the ester groups in 7) to 1658 cm −1 (characteristic of the carboxylate functions in 1). This was further confirmed with the results obtained by 13 C CPMAS NMR. As clearly depicted in Fig.…”

“…2A), the peak at 3289 cm −1 corresponding to the vibration of the C-H bond in the terminal alkynyl -CuC-H moiety of 6 disappeared following the "click" reaction, and a very strong signal was generated around 1707 cm −1 , relating to the carbonyl group of the ester functions of 7. The successful conversion of 6 to 7 was further affirmed by 13 C cross polarization magic angle spinning (CPMAS) NMR, an excellent non-destructive method for the in situ analysis of the solid phase reaction. [22][23][24] As illustrated in Fig.…”

“…Silica gel (200-300 mesh) used for flash chromatography was purchased from Qing Dao Hai Yang Chemical Industry Co. (China). 1 H NMR and 13 C NMR spectra were recorded at 300 MHz and 75 or 150 MHz respectively on Varian Mercury-VX300 and VX600 spectrometers. Chemical shifts are reported in parts per million ( ppm) with TMS as an internal reference.…”

“…Based on this rationale, we report here the synthesis, characterization and biological validation of the so-devised affinity resin 1 that can selectively and specifically recognize, retain and separate the 2-OG binding protein NtcA, a known 2-OG receptor involved in regulating the carbon/nitrogen balance and heterocyst differentiation in diazotrophic cyanobacteria. 4,[12][13][14][15]17 To our knowledge, this is the first example of an affinity resin using a 2-OG mimic tag validated with a known 2-OG receptor. 18 This affinity system hence holds great promise in searching for 2-OG receptors or 2-OG binding proteins involved in signal transduction and metabolic regulation.…”

“…Furthermore, 2-OG is a small molecule, and any structural modification with relatively large functional moieties will inevitably modify binding characteristics towards 2-OG receptors. 4,[12][13][14][15] Taking all these into consideration, we opted for the nonmetabolisable 2-OG analogue, 2,4-dimethylenepentanedioic acid (DMPA), as the 2-OG tag to construct the affinity resin 1 (Scheme 1) for its structural resemblance to, and resulting ability to mimic, 2-OG in signaling. 14 In addition, it is practical for chemical modification and conjugation of DMPA onto the solid support.…”