Study on morphology, pathogenicity, and genetic variability of Beauveria bassiana isolates obtained from Boophilus microplus tick

Abstract

Fifty isolates of Beauveria bassiana (Balsamo) Vuillemin, 1912 (Ascomycota: Clavicipitaceae) were analyzed by morphology, for their pathogenic potential to Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae) larvae, and by Random Amplified Polymorphic DNA-Polymerase Chain Reaction technique. Morphological analysis demonstrated that isolates present characteristics compatible to those described for B. bassiana in the literature. Virulence test demonstrated that all isolates present lethal effect on larvae and that the lethal concentration varies among isolates. The most virulent isolate was the only one obtained from human infection, which was also the only isolate presenting synnemata. The study on genetic variability among the isolates allowed the identification of 23 electrophoretic profiles. The established groupings suggest that most of the isolates obtained from B. microplus of the same locality present low genetic variation. In this way, the data in the present study will contribute to a meticulous characterization of these B. bassiana isolates.

Characterization and activity of entomopathogenic fungi isolates against “paraguay tea ampul” (Gyropsylla spegazziniana) (Lizer & Trelles) (Hemiptera: Psyllidae)

“…In line with this suggestion, high genetic variability has been demonstrated among entomopathogenic fungi, and the level of polymorphism can be highly variable even within the same species (FERNANDES et al, 2006). Thus, the isolates of genus Beauveria presented higher activity against the ampul, and it can be concluded that the Unioeste 44 isolate show great potential for the control of G. spegazziniana.…”

Variabilidade genética de isolados de Beauveria spp. e virulência ao cascudinho Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae)

“…Tais divergências podem ser explicadas pelo elevado grau de variabilidade genética exibida pelos fungos entomopatogênicos, pois mesmo dentro de uma determinada população, o nível de polimorfismo entre os isolados pode ser altamente variável (SOSA-GÓMEZ; TIGANO; ARANTES, 1998;FERNANDES et al, 2006). Além disso, os isolados utilizados no referido trabalho não foram os mesmos utilizados no presente estudo.…”

Antimicrobial activity of Stomoxys calcitransagainst Beauveria bassiana sensu lato isolates

“…Different isolates have been used to control arthropods that affect livestock (FERNANDES et al, 2006) and crops (ALVES, 1998). Other studies have assessed this species' genetic variability and geographic distribution (FERNANDES et al, 2006;FERNANDES et al, 2009), interaction with chemical insecticides (McCOY & TIGANO-MILANI, 1992), tolerance to stress conditions and performance (FERNANDES et al, 2007), enzymatic activity, production of toxins and action on beneficial arthropods, animals and humans (ZIMMERMANN, 2007).…”