Study on phosphate uptake of the marine cyanophyte Synechococcus sp. NIBB 1071 in relation to oligotrophic environments in the open ocean

Ikeya, Tomohiko; Ohki, K.; Takahashi, Masayuki; Fujita, Yoshihiko

doi:10.1007/s002270050160

Cited by 34 publications

(36 citation statements)

References 37 publications

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“…Our ATP uptake half saturation constants are likewise much lower than the values they measured for dCTP uptake. In contrast, our DIP uptake K s values are $1.7-and 4-10-fold higher than those presented in Ikeya et al (1997) for DIP-replete and DIP-limited cells, respectively.…”

contrasting

confidence: 97%

“…Half-saturation constants for P uptake from Donald et al (1997) and Ikeya et al (1997) are compared with the results of our study in Table 3. The DIP uptake K s values in our study for DIP-limited and DIP-replete cultures are one to two orders of magnitude lower than the comparable values obtained by Donald et al (1997) for the same strain.…”

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confidence: 99%

“…Direct comparisons between these studies are problematic due to unknown differences in the degree of P limitation and different culturing methods; Donald et al (1997) used batch cultures, Ikeya et al (1997) used chemostats, and our study used semi-continuous methods. Ikeya et al (1997) also used a different Synechococcus isolate.…”

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confidence: 99%

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Phosphate and ATP uptake and growth kinetics in axenic cultures of the cyanobacteriumSynechococcusCCMP 1334

Zhang

Feng

et al. 2006

European Journal of Phycology

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Phosphorus potentially limits the growth and productivity of the unicellular cyanobacterium Synechococcus in many oligotrophic oceans. Both dissolved inorganic phosphorus (DIP) and dissolved organic phosphorus (DOP) could be important P sources in these regimes. We compared DIP and adenosine tri-phosphate (ATP) uptake and growth kinetics using axenic semi-continuous cultures of Synechococcus CCMP 1334 grown on both P substrates, under both P-limited and P-replete conditions. Molar C:total P ratios and N:total P ratios were 417-431 and 65-68, respectively, under severely P-limited growth conditions for both P sources, but were near Redfield values at >1.0 mM P as either DIP or ATP, and were inversely proportional to growth rates. Synechococcus grown on ATP as the sole P-source have the same maximum growth rates, lower half-saturation constants for growth, and similar cellular P quotas compared with cultures grown on DIP. DI 33 P and 33 P-ATP uptake experiments confirmed that both can be used by Synechococcus, with relative uptake rates depending on prior growth history. DIP-limited cultures assimilated ATP faster than DIP, while DIP-replete cultures took up DIP more rapidly. In contrast, for Synechococcus grown under ATP-limited conditions, there is no priority for the utilization of ATP or DIP, but ATP-replete cultures have a greater ability to use DIP. Like other phytoplankton, kinetic constants for Synechococcus DIP uptake and cellular P quotas, C:P and N:P ratios of DIP-grown cells differ when total cellular and intracellular P pools are compared, suggesting a need to re-evaluate these parameters to account for abiotic phosphate scavenging onto cell surfaces. DIP half-saturation constants for growth of this Synechococcus strain would not support observed natural growth rates at the nanomolar DIP concentrations found in most oligotrophic regimes, and hence populations in these areas probably rely primarily on DOP.

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Phosphate and ATP uptake and growth kinetics in axenic cultures of the cyanobacteriumSynechococcusCCMP 1334

Zhang

Feng

et al. 2006

European Journal of Phycology

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“…A chemostat-type continuous-culture device equipped with a cylindrical culture vessel (500 ml) was used for all experiments (Ikeya et al, 1997). The cells from the seed culture were inoculated into the culture vessel of the continuous-culture device filled with fresh medium.…”

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confidence: 99%

Diazotrophy under continuous light in a marine unicellular diazotrophic cyanobacterium, Gloeothece sp. 68DGA

et al. 2008

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Nitrogenase is extremely sensitive to molecular oxygen (O 2 ), and unicellular diazotrophic cyanobacteria separate nitrogen (N 2 )-fixation and photosynthesis to protect nitrogenase from O 2 produced by photosynthesis. When grown under 12 h light/12 h dark cycles (LD), the marine unicellular diazotrophic cyanobacterium Gloeothece sp. 68DGA expressed the nitrogenase protein and its activity (acetylene reduction activity) only during the dark phase. However, this strain was able to grow diazotrophically under continuous light (CL). To determine whether nitrogenase synthesis and N 2 -fixation are temporally separated from photosynthesis in the Gloeothece cells that have fully acclimated to CL, the proportion of cells containing nitrogenase (the Fe-protein of nitrogenase) in the culture was measured using an immunocytochemical technique. Cells were grown in a continuous-culture device to maintain constant cell density. Under LD, the cells showed diurnal oscillation of nitrogenase activity, photosynthesis, respiration and the expression and the abundance of the Fe-protein. The oscillation was gradually reduced after the transfer of the cells to CL, and was lost after 23-25 days of cultivation under CL. In CL-acclimated cultures, the Fe-protein was always detected in about 94 % of the cells, although the nitrogenase activity was about one-third of the maximum activity in LD-acclimated cultures. These results suggest that synthesis of nitrogenase proceeds without diurnal oscillation in the CL-acclimated cells of Gloeothece sp. 68DGA. As the respiration rate in CL-acclimated culture was as high as the maximum rate observed in LD-acclimated culture, O 2 -uptake mechanism(s) may have been upregulated to maintain low intracellular pO 2 .

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“…With respect to phosphorus limitation, experiments conducted with a marine Synechococcus indicated that the ambient inorganic phosphorus concentration in the surface ocean of 0.5 Â 10 À9 M would be sufficient to maintain a growth rate of 1.0 d À1 (Ikeya et al, 1997). It has been demonstrated that in addition to inorganic N and P, a variety of organically bound N (Wafar et al, 1995) and P (Bjorkman and Karl, 1994;Li, 1998) is present.…”

Section: àmentioning

confidence: 99%

Physiological responses of three species of marine pico-phytoplankton to ammonium, phosphate, iron and light limitation

Timmermans

Wagt

Veldhuis

et al. 2005

Journal of Sea Research

106

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Physiological responses of three species of marine pico-phytoplankton to ammonium, phosphate, iron and light limitation Timmermans, K.R.; Wagt, B. van der; Veldhuis, M.J.W.; Maatman, A.; de Baar, Henricus Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. AbstractExperiments were conducted with three species of marine pico-phytoplankton: Synechococcus sp. (CCMP 839), Pelagomonas calceolata (CCMP 1756) and Prasinomonas capsulatus (CCMP 1617) in order to collect physiological parameters for pico-phytoplankton to be utilised in Ocean Biogeochemical Climate Models. The main parameters to follow the effects of ammonium, phosphate, iron and light limitation were cell growth rates (A), half saturation constants for growth (K m ), N, P and Fe quota (per cell or per mol C), and photochemical quantum efficiency (F v /F m ).The nitrate and phosphate limitation experiments demonstrated that the small phytoplankton species could grow at low N and P concentrations. K m values were in the micro-molar (NH 4 + ) and sub-micro-molar (PO 4 3À ) range. N and P quota were in the femto-molar range per cell and varied from nutrient-deplete to nutrient-replete conditions. F v /F m values were only adversely affected at the lowest N and P concentrations in these experiments. In the Fe limitation experiments, it was shown that all three species were adversely affected only at extremely low Fe concentrations. Iron chelating agents had to be added to force the species in Fe limitation till ultimately growth stopped. K m values with respect to dissolved Fe were in the femto-molar range. Fe quota were in the low zepto-molar (10 À21 M) range per cell, and varied considerably from Fe limiting to Fe replete growth conditions. F v /F m values diminished only at the lowest iron concentrations. In the light limitation experiments, growth rates and photochemical quantum efficiencies were adversely affected only at irradiance levels below 10 Amol photons m À2 s À1 . These results indicate that the pico-phytoplankton species will hardly ever be completely stopped in their growth by NH 4 + , PO 4 3À, Fe or light (separately) under natural conditions. D

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Study on phosphate uptake of the marine cyanophyte Synechococcus sp. NIBB 1071 in relation to oligotrophic environments in the open ocean

Cited by 34 publications

References 37 publications

Phosphate and ATP uptake and growth kinetics in axenic cultures of the cyanobacteriumSynechococcusCCMP 1334

Phosphate and ATP uptake and growth kinetics in axenic cultures of the cyanobacteriumSynechococcusCCMP 1334

Diazotrophy under continuous light in a marine unicellular diazotrophic cyanobacterium, Gloeothece sp. 68DGA

Physiological responses of three species of marine pico-phytoplankton to ammonium, phosphate, iron and light limitation

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