Study on Physio-chemical Properties of plasma polymerization in C2H2/N2 plasma and Their Impact on COL X

Abstract

Nitrogen-containing plasma polymerization is of considerable interest for tissue engineering due to their properties on cell adhesion and mesenchymal stem cells (MSCs) response. In this study, low-pressure RF plasma of acetylene and nitrogen was used to deposit nitrogen-containing plasma polymerized coatings on several substrates. Deposition kinetics and surface characteristics of coatings were investigated in terms of RF power and gas flow ratio. OES was used to monitor the plasma process and investigate the relation between the film structure and plasma species. Presence of several bonds and low concentration of amine functional groups were determined using FTIR and Colorimetric methods. Contact angle goniometry results indicated about 30% increase in surface hydrophilicity. Stability of coatings in air and two different liquid environments was examined by repeating surface free energy measurements. Deposited films exhibited acceptable stability during the storage duration. Surface roughness measured by AFM was found to decrease with growing concentration of nitrogen. The deposition rate increased with increasing RF power and decreased with growing concentration of nitrogen. Zeta potential measurements of coatings revealed the negative potential on the surface of the thin films. Temporary suppression of collagen X in the presence of plasma coatings was confirmed by RT-PCR results.

Effect of Functional Groups of Self-Assembled Monolayers on Protein Adsorption and Initial Cell Adhesion

“…Surface modifications using various physical, chemical, and biological processes had been reported to circumvent such unwanted processes. − Surfaces of ceramics, polymers, and metals have been modified via physical (for example, plasma treatment) and chemical methods (for example, functionalization via self-assembled monolayers [SAMs], immobilized ligands, etc.) to carefully modulate protein adsorption, cell adhesion, and differentiation. − …”

Protein ion yield enhancement in matrix‐assisted laser desorption/ionization mass spectrometry after sample and matrix low‐pressure glow discharge plasma irradiation

“…Only powder samples were irradiated in the system. The aforementioned plasma system had previously been used in different fields of study 22–24 …”

“…The aforementioned plasma system had previously been used in different fields of study. [22][23][24]…”

Análisis reológico y estructural de líquidos iónicos de alto peso molecular

“…Con los resultados se observa que a menor tiempo de reacción hay un menor espesor. También, el cambio de espesor disminuye cuando la potencia aumenta, puede ser por el aumento en el flujo [58]. [11,59].…”

“…Aquellas películas que contienen este grupo funcional, en presencia con agua y oxígeno atmosférico, se oxidan para formar grupos funcionales como amidas [62]. Además, altas concentraciones de nitrógeno en las películas aumentan la solubilidad en disolventes polares (ejemplo en agua) o en otros solventes [58,60], y se ven influenciadas por pH ácidos al protonarse parcialmente las aminas primarias. Las películas pp-VIm muestran una concentración similar de aminas primarias en ambos métodos con la derivatizacion con TFBA, mientas que la concentración de aminas secundarias con TFAA muestran una pérdida de grupos en ambos métodos.…”