Study on plasma exosome biomarkers of pregnant women with intrahepatic cholestasis of pregnancy

Abstract

Background Elevated serum total bile acid level is currently the main index for clinical diagnosis of intrahepatic cholestasis of pregnancy, but the use of TBA as a detection index has certain limitations. The early diagnosis of ICP and new treatment options still need to be further strengthened.Methods: Plasma samples were collected, and exosomes were isolated. Key differential proteins were screened by bioinformatics methods. ELISA method was used to detect the concentration of the key differential protein cluster in plasma samples, and the ROC curve was drawn to nd out the best critical value.Results: There were 138 differentially expressed proteins between the ICP group and the normal group by quantitative analysis. Cluster protein was screened as a clinical validation index. The cluster protein concentration of plasma exosomes in the ICP group was signi cantly higher than that in the normal group (P<0.0001). ROC curve analysis showed that the best critical point for diagnosing ICP according to the plasma exosome cluster protein concentration of pregnant women was 255.28 ng/ml. In the ICP group, the best crucial point for predicting ICP with premature delivery is 286.72 ng/ml. Conclusion: the plasma exosome cluster protein concentration of ICP pregnant women is signi cantly higher than that of normal pregnant women. When the plasma cluster protein concentration of pregnant women is more remarkable than 255.28ng/ml, ICP can be diagnosed. When the plasma cluster protein concentration of pregnant women is higher than 286.72ng/ml, ICP pregnant women are more likely to have a premature birth.

Unique microRNA expression profiles in plasmic exosomes from intrahepatic cholestasis of pregnancy

“…Recent research has shown that the expression of microRNA is closely associated with the occurrence and progression of several pregnancy-related diseases, such as recurrent spontaneous abortion, gestational diabetes mellitus, gestational hypertension, ICP, etc. [ 12 – 14 ]. In addition, with the continuous accuracy of detection technology and the accessibility of serum microRNAs, circulating microRNAs have emerged as a kind of disease marker with diagnostic and prognostic potential [ 15 ].…”