Study on pre-fertilization barriers in the interspecific hybridization between Cucurbita pepo L. and C. moschata duchesne

Kaur, Barinder; Garcha, Karmvir Singh; Chahal, Gagandeep Kaur; Dhatt, Ajmer Singh

doi:10.5958/2395-146x.2021.00084.3

Cited by 3 publications

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“…Conventional pollination was successful, when C. pepo was used as a female parent, but with genotype specificity. Differences in pollen tube growth and style length of genotypes described variable fertilization observed between C. moschata and C. pepo crosses 9 .…”

Section: Discussionmentioning

confidence: 92%

“…Both of these species belongs to same family and have identical chromosome number (2n = 2x = 40), but hybridization among them is rarely successful due to cross-compatibility issues caused by both pre and post fertilization barriers 7 – 9 . Pre-fertilization barriers comprises low pollen viability, failure of pollen germination, variations in pollen tube and flower style length among the genotypes while post fertilization barriers include embryo or endosperm degeneration 9 , 10 . Attempts have been made to develop interspecific hybrids in the genus Cucurbita using conventional pollinations involving large number of diverse genotypes and/or embryo rescue approach with limited success 10 – 12 .…”

Section: Introductionmentioning

confidence: 99%

“…C. pepo has the limitations of short growing season (February to May), narrow adaptability, high temperature sensitivity and virus susceptibility (zucchini yellow mosaic virus, cucumber mosaic virus and watermelon mosaic virus), whereas, a related species C. moschata has broad window of cultivation (February to November), wider adaptability, high temperature tolerance, virus resistance and better seed production potential 5,6 . Hence, transfer of hull-less seed trait from C. pepo to C. moschata can pave the way to solve above-mentioned issues being faced in sub-tropical and tropical regions.Both of these species belongs to same family and have identical chromosome number (2n = 2x = 40), but hybridization among them is rarely successful due to cross-compatibility issues caused by both pre and post fertilization barriers [7][8][9] . Pre-fertilization barriers comprises low pollen viability, failure of pollen germination, variations in pollen tube and flower style length among the genotypes while post fertilization barriers include embryo or endosperm degeneration 9,10 .…”

mentioning

confidence: 99%

“…Hence, transfer of hull-less seed trait from C. pepo to C. moschata can pave the way to solve above-mentioned issues being faced in sub-tropical and tropical regions.Both of these species belongs to same family and have identical chromosome number (2n = 2x = 40), but hybridization among them is rarely successful due to cross-compatibility issues caused by both pre and post fertilization barriers [7][8][9] . Pre-fertilization barriers comprises low pollen viability, failure of pollen germination, variations in pollen tube and flower style length among the genotypes while post fertilization barriers include embryo or endosperm degeneration 9,10 . Attempts have been made to develop interspecific hybrids in the genus…”

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confidence: 99%

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Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Kaur

Garcha

Sandhu

et al. 2023

Sci Rep

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Hull-less seed trait is preferred by nut and oil industries worldwide for snacking and oil extraction as it evades the expensive decorticating (dehulling) process. This seed trait is available in C. pepo only, which has small seed cavity, sensitive to various biotic and abiotic stresses, and restricted to temperate regions for cultivation. Contrarily, the related species C. moschata has wider adaptability, disease tolerance and high seed yield. Therefore, attempt was made to transfer this trait into C. moschata through conventional pollination and ovule culture using four parents of hull-less C. pepo and six of hulled C. moschata. Through conventional approach, few viable F1 seeds (12–23) were obtained by using C. pepo as female parent, but in three crosses (HLP36 × HM1343, HLP36 × HM1022 and HLP44 × HM1022) only, whereas, its use as male parent was not successful. This incompatibility issue of reciprocals was resolved through ovule culture of C. moschata genotypes HM1343 and HM6711 after 17 to 19 days of pollination with C. pepo genotypes HLP53 and HLP72, respectively. The hybridity of interspecific crosses was confirmed through SSR markers (alleles inherited from both the parents), morphological characters and micromorphological leaf traits (differed from both the parents). The successful transfer through interspecific hybridization was further established with the presence of hull-less seed in fruits of F2 populations. Outcome of this study would pave the way for enhancing the productivity and multi-season cultivation of snack-seeded pumpkin even in subtropical and tropical regions.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Kaur

Garcha

Sandhu

et al. 2023

Sci Rep

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“…However, mutagenesis is random rather than directed breeding, and the induced mutations are frequently unstable, where the mutant traits disappear after several generations of reproduction [23,24]. Interspecific hybridization is a common method for breeding new cultivars [25,26]. With different hybrid combinations, new cultivars with stable excellent traits can be quickly Unlike many plants, the genus Curcuma lacks a genetic transformation system, which makes it difficult to achieve feasible goals in molecular breeding [18,19].…”

Section: Introductionmentioning

confidence: 99%

Cross-Compatibility in Interspecific Hybridization of Different Curcuma Accessions

Zhou

Tan

et al. 2023

Plants

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Curcuma is extensively cultivated as a medicinal and ornamental plant in tropical and subtropical regions. Due to the bright bract color, distinctive inflorescence and long blooming period, it has become a new favorite in terms of the urban landscape, potted flowers and cut flowers. However, little research on breeding new cultivars using traditional plant breeding methods is available on the genus Curcuma. In the present study, pollen viability and stigma receptivity evaluation were performed, and the genetic relationship of 38 Curcuma accessions was evaluated, then 5 C. alismatifolia Gagnep. (Ca), 2 C. hybrid (Ch), 2 C. sparganiifolia Gagnep. cultivars and 4 Curcuma native species were selected as parents for subsequent interspecific cross-breeding. A total of 132 reciprocal crosses were carried out for interspecific hybridization, including 70 obverse and 62 inverse crosses. Obvious discrepancies among fruit-setting rates were manifested in different combinations and in reciprocal crosses. Results showed that the highest fruit-setting rate (87.5%) was observed in the Ca combinations. There were 87 combinations with a fruit-setting rate of 0%, which meant nearly 65.9% was incompatible. We concluded that C. alismatifolia ‘Siam Shadow’ (Ch34) was suitable as a male parent and C. petiolata Roxb. (Cpet) was suitable as a female parent to improve the fruit-setting rates. The maximum number of seeds per fruit (45.4) was obtained when C. alismatifolia ‘Chiang Mai Pink’ (Ca01) was used as a female parent followed by C. attenuata Wall. ex Baker (Catt) (42.8) and C. alismatifolia ‘Splash’ (Ca63) (39.6) as male parents. The highest germination rate was observed for the Ca group followed by Catt and C. sparganiifolia ‘Maetang Sunrise’ (Csms). The germination rates of Ca accessions ranged from 58.2% (C. alismatifolia ‘Siam Scarlet’ (Ca06) as a male parent) to 89.3% (C. alismatifolia ‘Sitone’ (Ca10) as a male parent) with an average value of 74.0%. Based on the results of hybrid identification, all the individuals from the four combinations exhibited paternal-specific bands, indicating that the true hybrid rates of crossings were 100%. Our results would facilitate the interspecific hybridization and introduction of genetic variation from wild species into the cultivars in Curcuma in the future, which could be helpful in realizing the sustainable application in urban green areas.

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Characterization of rice straw lignin phenolics and evaluation of their role in pollen tube growth in Cucurbita pepo L.

Kaur

Chahal

2023

Natural Product Research

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Rice straw lignin was extracted via alkaline hydrolysis and structurally characterized using FT-IR and 1 H NMR spectra. Ethyl acetate extract of acid solubilized lignin was found to contain p-coumaric acid, ferulic acid and caffeic acid as major phenolic acids which were isolated and characterized using spectral data. Amides of isolated phenolic acids were synthesized by their reaction with propyl and butyl amines using microwave irradiation and analysed using spectral studies. Phenolic acids and amides were evaluated for their effect on pollen germination and tube growth in pumpkin. Pollen tube length was significantly increased with N-butyl-3-(3, 4-dihydroxyphenyl) acrylamide and N-butyl-3-(4hydroxyphenyl) acrylamide at 5 ppm concentration than the control. These results could be utilised in increasing pollen tube length of Cucurbita pepo while making interspecific cross between C. moschata and C. pepo in order to transfer hull-less character of C. pepo to virus resistant C. moschata genotypes. ExperimentalRice straw (var. Pusa Basmati 1121 (PB 1121)) was collected from the fields of Punjab Agricultural University, Ludhiana. The straw was dried under sun in order to remove any moisture. It was then milled and sieved (1mm) to get homogeneous powder of the straw which was then stored in air-tight container at room temperature. Determination of Biochemical Composition of Rice StrawDetergent System method given by Goering and Van Soest (1970) was used to determine the percent composition of biochemical contents i.e., cellulose, hemicellulose, lignin, acid insoluble ash (AIA), mainly silica. Extraction of lignin from rice strawFinely powdered rice straw was given dilute acid pre-treatment using 1% H 2 SO 4 (w/v) with solid: liquid ratio of 1: 25 (110°C, 20 min, 120 rpm) followed by its cooling to room temperature and filtering via vacuum filtration forming a solubilized fraction. Further, alkaline hydrolysis (5% NaOH, 110°C, 45 min) was performed on solid residue to extract lignin. The black liquor was allowed to undergo acidification using 5% H 2 SO 4 till a pH range of 6-7 followed by its centrifugation for 20 min at 5000 rpm after which the precipitates were separated byvacuum filtration in order to obtain silica. The filtrate was

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Study on pre-fertilization barriers in the interspecific hybridization between Cucurbita pepo L. and C. moschata duchesne

Cited by 3 publications

References 0 publications

Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Cross-Compatibility in Interspecific Hybridization of Different Curcuma Accessions

Characterization of rice straw lignin phenolics and evaluation of their role in pollen tube growth in Cucurbita pepo L.

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