Study on Salmonella Isolates from Fresh Milk of Dairy Cows in Selected Districts of Wolaita Zone, Southern Ethiopia

Asefa, Isayas; Legabo, Ermias; Wolde, Tsegaye; Fesseha, Haben

doi:10.1155/2023/6837797

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…Unlike the ndings of our study, a higher incidence of Salmonella spp were shows up in Wolaita, SNNPR, Ethiopia that revealed 9.3% among 151 raw milk samples 44 , in this region also another study showed that 8.3% occurrence of Salmonella species from 384 raw milk samples, they use also 45 .…”

Section: Discussioncontrasting

confidence: 92%

Bacteriological quality and antimicrobial resistance patterns of isolates from raw milk in dairy farms in Ethiopia

Sebsibe,

Mulugeta,

Legesse

et al. 2023

Preprint

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Background Food-borne illness has a significant impact on public health and the economy, affecting nearly 30% of the population in developing countries annually. Infections transmitted through milk can lead to acute and severe diseases. However, there is inadequate information available regarding the bacteriological profile and antimicrobial resistance of organisms present in raw milk. This study aimed to assess the bacteriological quality and antimicrobial resistance patterns of isolates from raw milk at different dairy farms found in Ethiopia. Methods A cross-sectional study was conducted, selecting 176 raw milk samples through a multi-stage sampling technique from August 2022- April 2023. Bacteriological tests, including Aerobic colony count, total coliform count, thermotolerant coliform count, Salmonella and Shigella detection, as well as Staphylococcus enumerations, were performed. Further bacterial identification was carried out using VITEK-2 compact, while the antimicrobial susceptibility test was determined by the Kirby-Bauer disc-diffusion technique. Data analysis was conducted using SPSS V.27, employing descriptive statistics and appropriate statistical tests. Resut The mean aerobic colony count, total coliform, thermo-tolerant coliform, E. coli, and Staphylococcus count was,3.2, 2.70, 2.69, 2.9, and 3.1 log10 cfu/ml respectively. E. coli (77%), Klebsiella spp. (5%), Salmonella spp. (1.4%), S. aureus (13.6%), S. saprophyticus (9.8%) were also isolated. Gram-negative isolates showed sensitivity for Meropenem (96.8%) and Ciprofloxacin (89.9%) but resistant against Tetracycline (62.2%) and Streptomycin (62.2%). Staphylococcus isolates showed 95.1% Sensitivity for Levofloxacin and Gentamycin but 92.6% of isolates were resistant against Oxacillin. Extended-spectrum beta-lactamase production was detected in 16.6% of gram-negative isolates, and Methicillin resistance were observed in 30% of S. aureus isolates. Conclusion The results indicate that the overall quality of raw milk is poor, containing pathogenic and antibiotic-resistant organisms. This highlights the necessity for effective control measures to enhance production, storage, and processing practices.

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Section: Discussioncontrasting

confidence: 92%

Bacteriological quality and antimicrobial resistance patterns of isolates from raw milk in dairy farms in Ethiopia

Sebsibe,

Mulugeta,

Legesse

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Based on the parent’s response to the risk exposures, boiled or pasteurized milk reported to have been used by the household members including the ill child, two weeks prior to the onset of the diarrhea. Milk is reported among the potential source of Salmonella in many settings ( Maichin et al, 2013 ; Lubote et al, 2014 ; Silva et al, 2014 ; ElBaz et al, 2017 ; Asefa et al, 2023 ). Additionally, ST166 was detected in tap water from the street adjacent to the residence of the case linked with ST166.…”

Section: Discussionmentioning

confidence: 99%

High diversity of Salmonella spp. from children with diarrhea, food, and environmental sources in Kilimanjaro – Tanzania: one health approach

Hugho,

Kumburu,

Thomas

et al. 2024

Front. Microbiol.

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Salmonella is one of the most frequent causes of diarrhea globally. This study used a One Health approach to identify Salmonella species in children admitted with diarrhea and tested samples from the cases’ household environment to investigate their genetic similarity using whole genome sequencing. Surveillance of hospitalized diarrhea cases among children under 5 years was conducted in rural and urban Moshi Districts in the Kilimanjaro Region of Tanzania from July 2020 through November 2022. Household visits were conducted for every child case whose parent/caregiver provided consent. Stool samples, water, domestic animal feces, meat, and milk were collected and tested for Salmonella. Isolates were sequenced on the Illumina NextSeq platform. Multilocus Sequence Typing and phylogenetic analyses were performed to map the genetic relatedness of the isolates. Salmonella was isolated from 72 (6.0%) of 1,191 samples. The prevalence of Salmonella in children with diarrhea, domestic animal feces, food, and water was 2.6% (n = 8/306), 4.6% (n = 8/174), 4.2% (n = 16/382), and 17.3% (n = 39/225), respectively. Four (1.3%) of the 306 enrolled children had a Salmonella positive sample taken from their household. The common sequence types (STs) were ST1208, ST309, ST166, and ST473. Salmonella Newport was shared by a case and a raw milk sample taken from the same household. The study revealed a high diversity of Salmonella spp., however, we detected a Salmonella clone of ST1208 isolated at least from all types of samples. These findings contribute to understanding the epidemiology of Salmonella in the region and provide insight into potential control of foodborne diseases through a One Health approach.

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The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia

Beyene,

Nigatu,

Archila-Godinez

et al. 2024

Journal of Food Protection

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Study on Salmonella Isolates from Fresh Milk of Dairy Cows in Selected Districts of Wolaita Zone, Southern Ethiopia

Cited by 6 publications

References 30 publications

Bacteriological quality and antimicrobial resistance patterns of isolates from raw milk in dairy farms in Ethiopia

Bacteriological quality and antimicrobial resistance patterns of isolates from raw milk in dairy farms in Ethiopia

High diversity of Salmonella spp. from children with diarrhea, food, and environmental sources in Kilimanjaro – Tanzania: one health approach

The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia

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