In order to analyze the application effect of bridge pile foundation in Yushu permafrost area, this paper uses the research methods of theoretical analysis, model testing, and numerical simulation to collect soil samples in the Yushu area, study various indicators of frozen soil, simulate different frozen soil foundations in the Yushu area, and study different seismic waves under the influence of different temperatures. The seismic response mechanism of bridge pile foundations in the Yushu permafrost region under the influence of loading mode, soil material, and single pile in different permafrost regions is analyzed, and the dynamic response and mechanical nonlinear characteristics of displacement and deformation under different seismic forces are obtained. The simulation results show that under the influence of different temperatures, different seismic wave loading methods, and different soil quality single piles, the bridge pile foundation in the Yushu permafrost area has obvious seismic response laws and obvious mechanical nonlinear characteristics. Under the influence of permafrost material, most single piles have response coefficients of between 0 and 1.