Study on Selective Single Cell Isolation Method for Blood Cells

Abstract

In this study a method of selective isolation of a human leukemic cell was described. The method consisted of (1) use of a micro-picking-up chamber with a very small micropipette (18µ in inner diameter), and coverslip pressor, and (2) use of a 70x phase objective lens with oil immersion condenser. As an evidence of viability of the isolated cell, the nuclear rotation was demonstrated in a conditioned medium for five days culture.