Study on the anticancer effect and mechanism of action of reactive species in plasma‐activated solution against colorectal cancer cells

Interactions between pulsed electrical discharges and liquid dielectric materials is a growing research field with interests in fundamental discharge physics as well as in applications. Herein, we present an experimental study on the dynamics of nanosecond discharges in air with the presence of a water droplet with various electrical conductivities (EC) and at different applied voltages (Va). The discharges are characterized optically, by employing time-resolved ICCD imaging and optical emission spectroscopy, as well as electrically, by acquiring the current-voltage waveforms for every discharge. The results show that three modes of discharges can be obtained: i) streamer discharge between the cathode and the droplet, ii) streamer discharge between the cathode and the droplet as well as between the anode and the droplet, and iii) spark discharge that connects the two electrodes and propagates over the droplet. We find that the probability to obtain one of the three discharge modes is strongly related to the droplet’s EC as well as to Va. Although the ignition of the streamer is relatively insensitive to EC, its transition to a spark can be finely controlled by the droplet’s EC. The time-resolved ICCD images show that the discharge initiates in the gap between the cathode and the droplet, followed by ignition between the anode/ground electrode and the droplet. Then, an extinction phase is observed before the ignition of a secondary streamer, and depending on the conditions, the discharge may transition to a spark, i.e. a channel with high emission intensity. We find that the duration of each stage of discharge propagation as well as the corresponding emission (path and intensity) are sensitive to droplet’s EC. Finally, emissions from streamers (primary and secondary) as well as from sparks are analyzed using optical spectroscopy. We find that the emission from the streamers is dominated by the second positive system of N2, and that the droplet’s EC does not significantly influence the emission spectra nor the estimated rotational temperature of N2.

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Synergistic inhibitory effect of atmospheric pressure plasma and berberine on non‑small cell lung cancer cells via inducing apoptosis

Ming,

Lu,

Zhou

et al. 2024

Preprint

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Background Non-small cell lung cancer (NSCLC) is a type of lung cancer, the incidence and mortality rate have been high, and the use of monotherapy is easy to make patients develop tolerance. Atmospheric pressure plasma (APP) is an emerging technology for killing cancer cells in recent years, and there are fewer studies related to the combination of berberine and the mechanism of action has not been fully elucidated for NSCLC. The article primary goal is to investigate the effect of combination on NSCLC and its associated characterization. Methods and results Antiproliferative effects were detected by MTT assay and colony formation, and flow cytometry analysis of apoptosis and cycling showed that the combination synergistically induced apoptosis. Then, extracellular ROS levels and DCFH-DA-based kits examined intracellular ROS levels, and their effects on mitochondrial membrane potential were measured. Study reveals that co-induced apoptosis is associated with ROS accumulation. Subsequently, Western blotting (WB) detected the expression of EGFR, and the important signaling pathway proteins Ras / ERK and AKT / mTOR. Results showed that it could downregulation of EGFR protein expression and inhibit of activation of ERK/ AKT signaling pathways. Simultaneous wound healing assay and EMT marker detection were performed for the assessment of migration and EMT ability of NSCLC cells. Combination therapy inhibited migration and EMT of NSCLC cells. Conclusion The results of this study show that the combination can synergistically induce apoptosis of NSCLC by regulating ROS production. EGFR downregulation and AKT/ERK signaling pathway inhibition are linked to the synergistic effect.

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Study on the anticancer effect and mechanism of action of reactive species in plasma‐activated solution against colorectal cancer cells

Cited by 3 publications

References 36 publications

Synergistic inhibitory effect of atmospheric pressure plasma and berberine on non‑small cell lung cancer cells via inducing apoptosis

Synergistic inhibitory effect of atmospheric pressure plasma and berberine on non‑small cell lung cancer cells via inducing apoptosis

Electrical and optical characteristics of a nanosecond pulsed electrical discharge in air in contact with a water droplet for various electrical conductivities

Synergistic inhibitory effect of atmospheric pressure plasma and berberine on non‑small cell lung cancer cells via inducing apoptosis

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