Study on the association of p53 codon 72 polymorphisms with risk of gastric cancer in high incidence Hexi area of Gansu Province in China

Abstract

To investigate the possible association of P53 codon 72 Arg/Pro polymorphisms with risk of gastric cancer in the high incidence Hexi area of Gansu province in China. Blood samples from 140 patients with gastric carcinoma and 125 healthy controls were collected in Hexi area of Gansu province. Polymorphism of P53Arg72Pro was genotyped by PCR-TaqMan. For detection Helicobacter pylori infection, Warhin-Starry staining was used. Three kinds of polymorphisms of P53Arg72Pro were Arg/Arg, Arg/Pro, Pro/Pro. The frequencies in gastric cancer group were 15.7, 60.0, 24.3%, and the frequencies in healthy controls were 25.6, 54.4, 20.0%, respectively. P53 codon 72 Pro carrier genotype (Arg/Pro+Pro/Pro) increased risk of gastric carcinoma with an odds ratio 1.840 (95% CI: 1.006-3.387). Helicobacter pylori infection rate was 68.6% in patients group and 50.4% in healthy controls. Helicobacter pylori infection rate in gastric cancer patients was remarkably higher than that in the controls (OR: 2.147, 95% CI: 1.302-3.541, P=0.003). Stratification analysis showed that P53 codon 72 Pro carrier genotype with Helicobacter pylori infection was significantly higher in cases than that in the controls (OR: 4.182, 95% CI: 1.850-9.454). P53Arg72Pro polymorphisms could be a risk factor for gastric cancer in high incidence Hexi area of Gansu Province in China. P53 codon 72 Pro carrier genotype and Helicobacter pylori positive infection may have a synergistic effect on gastric cancer in high incidence Hexi area of Gansu Province in China.

p53 Codon 72 Polymorphism Interactions with Dietary and Tobacco Related Habits and Risk of Stomach Cancer in Mizoram, India

“…Zang et al, (2003) reported that Arg/Arg genotype is associated with high risk of gastric cancer. Ke-Xiang et al, (2012) in a Chinese population showed that Arg/Pro+Pro/Pro genotypes increased risk of gastric cancer. No significant association between p53 alleles and gastrointestinal cancers was reported by Mojtahedi et al, (2010).…”

Relationship betweenEGF,TGFA, andEGFRGene Polymorphisms and Traditional Chinese Medicine ZHENG in Gastric Cancer

“…The study of genetic variation could provide future implications for preventive and early intervention strategies. Many studies have suggested that GC development, treatment, and clinical outcome are associated with variations in several genes, including EGFR [48, 49], glutathione-S-transferase M1 and T1 [50], p53 [51], E-cadherin( CDH1 ) [52], and cyclooxygenase-2 [53]. We showed that lifestyle (such as meal duration) and clinical examination (such as the status of glutamic pyruvic transaminase) were significantly associated with GC ZHENG, and rs13689 in CDH1 is correlated with the GC ZHENG type [54].…”

Alterations of theTP53Gene in Gastric and Esophageal Carcinogenesis

“…In contrast, studies in Korea reported that Pro/Pro genotype was associated with increased risk of this neoplasm [95, 126]. While in a Chinese population, Ke-Xiang et al [123] showed that Arg/Pro + Pro/Pro genotypes increased risk of GC. Corroborating these data, a recent meta-analysis suggests that the Pro/Pro genotype was associated with several types of cancer, including GC [93].…”

“…The genetic susceptibility to gastric carcinogenesis related to TP53 polymorphisms may be attributed to several factors, including the accumulation and interaction of SNPs [124, 129], gene-environmental interactions [93], age [128, 131], H. pylori infection [123], bile or acid reflux, and smoking [137]. …”