Study on the Biocontrol Potential of Antifungal Peptides Produced by Bacillus velezensis against Fusarium solani That Infects the Passion Fruit Passiflora edulis

Wang, Caicheng; Ye, Xiujuan; Ng, Tzi Bun; Zhang, Wenjing

doi:10.1021/acs.jafc.0c06106

Cited by 25 publications

(21 citation statements)

References 50 publications

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“…Besides, W1 showed higher tolerance to a variety of proteases against F. oxysporum . These characteristics were better than the antifungal peptides produced by B. amyloliquefaciens S185, PT14, and B. velezensis HNAH 17806 ( Kim et al, 2015 ; Singh et al, 2021 ; Wang et al, 2021 ), indicating that W1 has superior application potential in the biocontrol of plant diseases.…”

Section: Discussionmentioning

confidence: 97%

Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases

et al. 2022

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A bacterium, Bacillus amyloliquefaciens W0101, isolated from the Arctic Ocean, showed potent antifungal activity against several plant pathogenic fungi. An antifungal peptide W1, with a molecular weight of approximately 2.4 kDa, was purified from the culture supernatant of the strain W0101 using ion-exchange chromatography and high-performance liquid chromatography. By analysis of Liquid Chromatograph-Mass Spectrometer, the peptide W1 was identified as a new antifungal peptide derived from the fragment of preprotein translocase subunit YajC. Further analysis revealed that W1 could disrupt the hyphae and spores of Sclerotinia sclerotiorum and inhibit its growth. W1 suppressed S. sclerotiorum and Fusarium oxysporum at a minimum inhibitory concentration of 140 and 58 μg/ml, respectively. The antifungal activity of W1 remained stable at 20–80°C or pH 6–11, with reduced activity at 100–110°C and pH 4–5, and under three protease treatments. Additionally, W1 also had a certain extent of metal ion resistance. These results therefore suggest that the peptide W1 from marine B. amyloliquefaciens W0101 may represent a new antifungal peptide with potential application in the biocontrol of plant diseases.

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Section: Discussionmentioning

confidence: 97%

Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases

et al. 2022

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“…citri (Etchegaray et al 2008 ). Fengycin from Bacillus velezensis achieved better inhibition of mycelial growth in Fusarium solani than chemical fungicides, and its activity remained stable even at high temperatures and low pH values (Wang et al 2021 ). Rahnella aquatilis JZ-GX1 produces volatile gases that antagonize Colletotrichum gloeosporioides , which causes Liriodendron chinense × tulipifera black spot, by containing 3-methyl-1-butanol and 2-methyl phenyl ether ethyl ether (Kong et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Medium optimization to analyze the protein composition of Bacillus pumilus HR10 antagonizing Sphaeropsis sapinea

Dai

Wang

et al. 2022

AMB Expr

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A previous study found that a biocontrol bacterium, Bacillus pumilus HR10, inhibited the Sphaeropsis shoot blight disease of pine, and the fermentation broth of HR10 strain contained protein antifungal substances. The optimal formulation of the fermentation medium for the antagonistic substance of B. pumilus HR10 was finally obtained by single-factor test, Packett-Burman test, steepest ascent test and Box-Behnken Design (BBD) response surface test, and the best formulation of the fermentation medium for the antagonistic substance of B. pumilus HR10 was 12 g/L corn meal, 15 g/L beef extract and 13 g/L magnesium sulfate, with a predicted bacterial inhibition rate of 89%. The fermentation filtrate of B. pumilus HR10 cultured with the optimized medium formulation was verified to have an inhibition rate of (87.04 ± 3.2) % on the growth of Sphaeropsis sapinea by three replicate tests. The antagonistic crude protein of B. pumilus HR10 were further isolated and identified using HiTrap Capto Q strong Ion-Exchange Chromatography and LC–MS-MS, and it was speculated that glycoside hydrolase (Ghy), beta-glucanase (Beta), arabinogalactan endonuclease β-1,4-galactanase (Arab), and immunosuppressant A (ImA) are proteins with antagonistic activity against S. sapinea in the B. pumilus HR10.

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“…A recent study indicated that B. velezensis effectively prevented F. solani infection in passion fruits ( Wang et al, 2021 ). B. velezensis strains with secondary-metabolite-secretion ability could inhibit F. solani and oomycetes, and these strains were reported to serve plant growth–promoting rhizobacteria ( Cheffi et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease

Wang

et al. 2021

Front. Microbiol.

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Wilt disease of roselle (Hibiscus sabdariffa L.) is common in Taiwan; however, the causative agent remains unknown. The stems of wilted roselle are browned, slightly constricted, and covered by white aerial hyphae, suggesting that potential pathogens may originate from soil. To identify the potential pathogens, we conducted a rhizosphere microbiota survey in phenotypically healthy and diseased plants through fungal internal transcribed spacer (ITS) and bacterial 16S rRNA amplicon sequencing for uncovering the microbial compositions in the roselle rhizosphere. The fungal family Nectriaceae exhibited significantly higher abundance in diseased rhizospheres than in healthy rhizospheres, and this bacterial community was more specific to geography (i.e., plot-dependent) than to rhizosphere disease status. However, a few bacterial groups such as Bacilli were associated with the healthy rhizosphere. Fusarium species were the most dominant species of Nectriaceae in the survey and became the main target for potential pathogen isolation. We successfully isolated 119 strains from diseased plants in roselle fields. Koch’s postulates were used to evaluate the pathogenicity of these strains; our results indicated that Fusarium solani K1 (FsK1) can cause wilting and a rotted pith in roselles, which was consistent with observations in the fields. This is the first demonstration that F. solani can cause roselle wilt in Taiwan. Furthermore, these newly isolated strains are the most dominant operational taxonomic units detected in ITS amplicon sequencing in diseased rhizospheres, which serves as further evidence that F. solani is the main pathogen causing the roselle wilt disease. Administration of Bacillus velezensis SOI-3374, a strain isolated from a healthy roselle rhizosphere, caused considerable anti-FsK1 activity, and it can serve as a potential biocontrol agent against roselle wilt disease.

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Study on the Biocontrol Potential of Antifungal Peptides Produced by Bacillus velezensis against Fusarium solani That Infects the Passion Fruit Passiflora edulis

Cited by 25 publications

References 50 publications

Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases

Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases

Medium optimization to analyze the protein composition of Bacillus pumilus HR10 antagonizing Sphaeropsis sapinea

Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease

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