Study on the biodegradation of crude oil by free and immobilized bacterial consortium in marine environment

Abstract

Five strains of bacteria, namely, Exiguobacterium sp. ASW-1, Pseudomonas aeruginosa strain ASW-2, Alcaligenes sp. ASW-3, Alcaligenes sp. ASS-1, and Bacillus sp. ASS-2, were isolated from the Zhejiang coast in China. The mixed flora of the five strains performed well with degrading 75.1% crude oil (1%, w/v) in 7 days. The calcium alginate—activated carbon embedding carrier was used to immobilize bacterial consortium. Immobilized cells performed better than free ones in variations of environmental factors containing incubated temperature, initial pH, salinity of the medium and crude oil concentration. The degradation process of crude oil by immobilized bacteria was accelerated compared with that of the free ones. Bacterial consortium showed better performance on biodegradation of normal alkanes than that of PAHs. Improvement of immobilization on the biodegradation efficiency of normal alkanes (31.9%) was apparently high than that of PAHs (1.9%).

Biological removal of crude oil with the use of native bacterial consortia isolated from the shorelines of the Caspian Sea

“…There was 51.9% crude oil (2% v/v) degradation after 7 days of incubation according to Adam [51]. A study by Chen et al [52] showed about 68% of biodegradation efficiency of crude oil (2% v/v) after 1 week of cultivation. Diesel degradation abilities by Staphylococcus sp., Pseudomonas sp.…”

Enhancement of Crude Oil Biodegradation by Pseudomonas reinekei MT1

“…Also Al-Wasify and Hamed (2014) obtained 77.8% of crude oil degradation by using Pseudomonas auroginosa after 28 days at 30ºC. Chen et al, (2017) found that optimum temperature for oil degradation with free and immobilized cells of Pseudomonas auroginosa ASW-2 was 30ºC.…”

Comparison of 2- and 3-compartment electrodialytic remediation cells for oil polluted soil from northwest Russia

“…The observed differences in the influence of variables and removal efficiencies between the 2-compartment and 3-compartment cells could be related to differences in the EDR process in the two designs. Acidification occurs faster in the 2-compartment cell, resulting in lower pH levels, that in turn may retard the natural bioremediation of oil in the soil [32]. This may also indicate that the observed removal of THC from the soil is related to degradation rather than solubilisation into the liquid phase.…”