Study on the bioelectrochemistry of a horseradish peroxidase-gold nanoclusters bionanocomposite

He, Fang; Bu, Lijuan; Fu, Yingchun; Tan, Yueming; Chen, Chao; Li, Yunlong; Xie, Qingji; Yao, Shouzhuo

doi:10.1016/j.jelechem.2017.03.033

Cited by 12 publications

(2 citation statements)

References 53 publications

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“…It is noteworthy that many researchers have developed enzyme-stabilized AuNC-based electrochemical biosensors. 27,28 Glucose is one of the most important and widely used targets in enzyme-based biosensors as altered glucose levels can cause fatal diseases such as diabetes mellitus. 29,30 Glucose is oxidized by glucose oxidase (GO X ) to produce gluconic acid and hydrogen peroxide (H 2 O 2 ).…”

Section: Introductionmentioning

confidence: 99%

“…Enzyme-stabilized AuNCs have higher conductivity than bare enzymes since they can increase the electron transfer rate between the enzyme and electrode, even when many enzymes are immobilized on the electrode. , From this perspective, enzyme-stabilized AuNCs are very interesting and excellent materials for the development and/or optimization of electrochemical biosensors. It is noteworthy that many researchers have developed enzyme-stabilized AuNC-based electrochemical biosensors. , …”

Section: Introductionmentioning

confidence: 99%

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Gold Nanoclusters with Two Sets of Embedded Enzyme Nanoparticles for Applications as Electrochemical Sensors for Glucose

Lee

Choi

Shin

et al. 2023

ACS Appl. Nano Mater.

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Enzyme-based electrochemical biosensors have been widely used because of their sensitivity, rapidity, and highly target-specific reactions. However, the immobilized enzymes can hinder electron transfer on the surface of the electrode and can decrease the sensitivity of electrochemical biosensors. To overcome this problem, we synthesized conductive gold nanoclusters with two sets of embedded enzyme nanoparticles, which are called gold nanocluster-embedded dual-enzyme nanoparticles (AuNC-DENPs) composed of a glucose oxidase-stabilized gold nanocluster (GOX-AuNC) and a horseradish peroxide-stabilized gold nanocluster (HRP-AuNC). Moreover, the application of these nanoparticles as an enzyme-based highly sensitive electrochemical sensor was investigated. Owing to the effect of AuNCs, these nanoparticles have good conductivity compared to bare protein nanoparticles. In addition, the synthesized AuNC-DENPs enabled the combination of a two-enzyme cascade reaction, in which the GOX-AuNC component of the nanoparticle oxidized glucose to generate hydrogen peroxide, which then reacted with the adjacent HRP-AuNC component on the nanoparticle. Given the proximity of the two enzyme components in a single nanoparticle, the AuNC-DENPs markedly reduced the diffusion and decomposition of H2O2 during the cascade reaction and showed an enhanced catalytic reaction compared to a mixture of enzymes. As a result, the biosensor exhibited high sensitivity (18,944 μA/mM cm2) and could detect very low concentrations of glucose ranging from 5 to 320 nM and a LOD of 2.58 nM. In addition, by analyzing nontarget materials and serum together with glucose, it was confirmed that the sensor has good selectivity for glucose.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gold Nanoclusters with Two Sets of Embedded Enzyme Nanoparticles for Applications as Electrochemical Sensors for Glucose

Lee

Choi

Shin

et al. 2023

ACS Appl. Nano Mater.

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Sensor‐Based Devices for Trace Evidence

Dey

Rao

Rawtani

2023

Modern Forensic Tools and Devices

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Kinetics of Oxygen Reduction by a Beta Barrel Heme Protein on Hyrid Bioelectrodes

2020

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Efficient catalysts are required to activate O2 at low overpotentials with improved reaction kinetics. The kinetics of electrocatalytic oxygen reduction by a β‐barrel, the nitrobindin heme protein (NBHP), is investigated using protein‐film electrochemistry. Structure‐guided strategies were developed to covalently attach NBHP and enable efficient interfacial electron transfer (ET) between the protein and electrode. The reduction of Fe(III) to Fe(II) with a rate constant ks of 25 s−1 is invoked as the rate‐limiting step of the catalytic process, as opposed to a proton‐coupled electron transfer (PCET) process. Further kinetic analyses revealed a complete 4e− reduction of O2 to H2O at lower pH, attributable to a higher H+ availability and more facile reduction of the heme in acidic conditions compared to the basic conditions where an incomplete 2e− reduction to H2O2 was observed. The rate constants kORR, k0, and the turnover numbers demonstrate that NBHP is an efficient biocatalyst for O2 electrolysis.

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Study on the bioelectrochemistry of a horseradish peroxidase-gold nanoclusters bionanocomposite

Cited by 12 publications

References 53 publications

Gold Nanoclusters with Two Sets of Embedded Enzyme Nanoparticles for Applications as Electrochemical Sensors for Glucose

Gold Nanoclusters with Two Sets of Embedded Enzyme Nanoparticles for Applications as Electrochemical Sensors for Glucose

Sensor‐Based Devices for Trace Evidence

Kinetics of Oxygen Reduction by a Beta Barrel Heme Protein on Hyrid Bioelectrodes

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