STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides

Dewi, Ariyanti Suhita; Hadi, Tri Aryono; Januar, Hedi Indra; Pratitis, Asri; Chasanah, Ekowati

doi:10.15578/squalen.v7i3.4

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…Meanwhile, similar activities were exhibited by the CH 3 OH extracts of the sponges from the genera Aaptos, Dactylia, Dictyonellida, Acanthella, Axinella, Cribrochalina, Neofibularia, and Rhabdastrella. Various compounds have been detected in Indonesian sponges, particularly those from Karimunjawa and Seribu Islands Marine National Parks, such as the cytotoxic aaptamine from Aaptos sp., and betaines, and/or various brominated compounds from Axinella sp. (Januar et al 2007;Dewi et al 2012), the source of which may in fact be their symbiotic bacteria (Uria & Piel, 2009).…”

Section: Bioactivity Potential Of Sponges and Soft Coralsmentioning

confidence: 99%

Influence of Anthropogenic Pressures on the Bioactivity Potential of Sponges and Soft Corals in the Coral Reef Environment

Januar

Chasanah²,

Tapiolas³

et al. 2015

Squalen Bull. Marine Fisheries Postharvest Biotech.

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The wealth of marine sponges and soft corals in Indonesian waters represents a rich source of natural products. However, anthropogenic pressures potentially decrease diversity in coral reefs. Presented here are trends for tropical sponge and soft coral biodiversity and their bioactivity potential under the influence of increasing anthropogenic pressures. Samples were collected along transects (near, mid, and far) at Karimunjawa and Seribu Islands Marine National Parks and environmental parameters (salinity, pH, dissolved oxygen (DO), phosphate, nitrate, and ammonia), sponge and soft coral biodiversity, and the bioactivity potential of those organisms (50% Growth Inhibition (GI50) of cancer cell lines H460-Lung, MCF7-Breast, and SF268-CNS) are compared. The environmental conditions and biodiversity were found to be significantly different between groups of sampling sites (P<0.05). Canonical Discriminant Analysis (CDA) revealed DO was the discriminant factor driving the separation between groups (90.1%). Diversity tended to be higher in the Far group with strong and significant relation to DO (R= 0.611, P<0.05) and ammonia (R = -0.812, P<0.05). The CDA also showed that an increase in bioactivity (low % GI50) of sponge and soft coral extracts was related to a canonical function (57.21%) consisting of high DO, high pH, and low nutrients. These findings indicate the production of bioactive compounds is related to diversity and complexity of coral reef systems. Therefore, strategies for marine protection by mitigating the impacts of anthropogenic pressures needs to be optimized in order to conserve the overall environment and sustain its natural bioactivity potential indefinitely.

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Section: Bioactivity Potential Of Sponges and Soft Coralsmentioning

confidence: 99%

Influence of Anthropogenic Pressures on the Bioactivity Potential of Sponges and Soft Corals in the Coral Reef Environment

Januar

Chasanah²,

Tapiolas³

et al. 2015

Squalen Bull. Marine Fisheries Postharvest Biotech.

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“…Eluent used was 20% acetonitrite/H O (1% TFA) at a flow rate 0.2 mL 2 -1 min . Concentration of aaptamine and isoaaptamine were determined according to Dewi et al (2012) 53 and 4.78, respectively. This was supported by Simpson's Index, in which the D value of the bacterial population from Aaptos sp.…”

Section: Max Maxmentioning

confidence: 99%

Analysis of Bacterial Community Associated with Aaptos sp. from Rote and Seribu Islands

et al. 2013

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Aaptos sp., one of marine sponges that can be found abundantly in Indonesian waters, has been reported to produce bioactive metabolite against tumor, pathogenic microbes, and Herpes Simplex Virus type I (HSV-1) (Coutinho et al. 2002). It was detected that the compounds produced were alkaloids, e.g aaptamin, aaptosin and isoaaptamine; the latter compound showed potent activity against Human Immunodeficiency Virus type I (HIV-1) (Gul et al. 2006). Aaptos sp. was also been reported to produce sterol compounds (Rachmat and Muniarsih 2001), and other novel aaptamine alkaloids possesing various biological activities, including cytotoxic against murine lymphoma L5178Y cell line, antiviral, antimicrobial, antifungal, antiparasitic, α-adrenergic antagonistic, radical scavenging, and antifouling activity (Pham et al. 2013).Sponge such as Aaptos sp. is very rich in microorganism including bacteria associated within their body. About 40 % of sponge body is bacteria, and its role is very significant in the sponge metabolism (Taylor et al. 2007). The sponge produces similar metabolites from their associated bacteria (Radjasa et Aaptos sp. is a marine sponge that could produce bioactive compounds such as aaptamin, aaptosin, and isoaaptamin which have activities as antitumor, antimicrobial, and antiviral. Community of bacteria associated with the sponge might correlate with production of those bioactive compounds and be affected by water environment where the sponge grow. The presence of anthropogenic stressor such as pollutans might become a burden to the waters where the biota grown and could affect the microbial biodiversity in the sponge and its active metabolite produced. The objective of this research was to analyze bacterial community associated with Aaptos sp. from Rote Island and Seribu Islands, using T-RFLP method. The results showed that bacterial community associated with Aaptos sp. from both sampling sites shared 40.81% similarity in which they were dominated by the same bacteria class of Actinobacteria, Flavobacteria, α-proteobacteria, δ-proteobacteria, and γ-proteobacteria. The bacteria collected from Rote island were more highly distributed and diverse than those from Seribu Islands. A total of 23 classes of microorganism were identified in Rote Island waters, while in Seribu Islands was 14 classes of microorganism. The presence of Actinobacteria and Proteobacteria in Aaptos sp., is allegedly involved in the production of secondary metabolites.

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STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides

Cited by 2 publications

References 18 publications

Influence of Anthropogenic Pressures on the Bioactivity Potential of Sponges and Soft Corals in the Coral Reef Environment

Influence of Anthropogenic Pressures on the Bioactivity Potential of Sponges and Soft Corals in the Coral Reef Environment

Analysis of Bacterial Community Associated with Aaptos sp. from Rote and Seribu Islands

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