Study on the effect of magnetic field treatment of newly isolated Paenibacillus sp.

Abstract

BackgroundSymbiotic nitrogen fixation in plants occurs in roots with the help of some bacteria which help in soil nitrogen fertility management. Isolation of significant environment friendly bacteria for nitrogen fixation is very important to enhance yield in plants.ResultsIn this study effect of different magnetic field intensity and treatment time was studied on the morphology, physiology and nitrogen fixing capacity of newly isolated Paenibaccilus sp. from brown soil. The bacterium was identified by 16S rDNA sequence having highest similarity (99%) with Paenibacillus sp as revealed by BLAST. Different magnetic intensities such as 100mT, 300mT and 500mT were applied with processing time of 0, 5, 10, 20 and 30 minutes. Of all these treatment 300mT with processing time of 10 minutes was found to be most suitable treatment. Results revealed that magnetic treatment improve the growth rate with shorter generation time leading to increased enzyme activities (catalase, peroxidase and superoxide dismutase) and nitrogen fixing efficiencies. High magnetic field intensity (500mT) caused ruptured cell morphology and decreased enzyme activities which lead to less nitrogen fixation.ConclusionIt is concluded that appropriate magnetic field intensity and treatment time play a vital role in the growth of soil bacteria which increases the nitrogen fixing ability which affects the yield of plant. These results were very helpful in future breading programs to enhance the yield of soybean.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-015-0083-9) contains supplementary material, which is available to authorized users.

Using of Magnetic Water Technology for the Management of Brown Rot Disease of Potato

“…B11. In addition, Li et al (2015) indicated that the treatment of 300 mT for 10 minutes improved Paenibacillus sp. growth.…”

“…(Fijałkowski et al, 2015) mentioned that the enhancement of bacterial viability may be due to the activation of bacterial metabolic activity that stimulates the growth rate and biofilm formation. Li et al (2015) and Ren et al (2018) revealed that the stimulation of bacterial growth may also due to the increasing of bacterial enzymatic activities.…”

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“…Medan magnet dilaporkan dapat mempengaruhi aktivitas enzim peroxidase, catalase dan superoxide dismutase (Lie, 2015).…”

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