Study on the efficacy of Leptospira vaccines developed from serovars isolated from Trinidad and comparison with commercial vaccines using a hamster model

Suepaul, Sharianne; Carrington, Christine V. F.; Campbell, Michael H.; Borde, G.; Adesiyun, Abiodun A.

doi:10.1016/j.vaccine.2010.06.019

Cited by 21 publications

(30 citation statements)

References 32 publications

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“…Contar con aislamientos de cepas pertenecientes a diferentes serogrupos y serovares como candidatos vacunales, caracterizados por una alta virulencia, representativos de los serogrupos que estén circulando en una región determinada, simplificaría gradualmente la confección de una vacuna antileptospirósica hecha a la medida (6,8,13,29,30). Por tanto una evaluación más completa de la capacidad de candidatos vacunales para inducir una respuesta inmunoprotectora.…”

Section: Discussionunclassified

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Estudio anatomopatológico de aislados de Leptospira spp., provenientes de Nicaragua en Mesocricetus auratus como biomodelo

et al. 2013

Rev MVZ Córdoba

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RESUMENObjetivo. El objetivo de este trabajo fue caracterizar en el biomodelo Mesocricetus auratus la sintomatología y lesiones anatomopatológicas que provocan 5 aislados clínicos de Leptospira spp., provenientes de Nicaragua. Materiales y métodos. Con este fin se inocularon 50 hámster por vía i.p con 1mL del cultivo de cada una de las cepas en fase exponencial teniendo una concentración celular de 7.5 x 10 6 leptospira/mL (10 animales por cepa), evaluándose signos de la enfermedad, mortalidad durante 14 días, lesiones anatomopatológicas macroscópicas y microscópicas mediante tinción con hematoxilina-eosina y tinción de Warthyn Starryn. Resultados. Todas las cepas presentaron alta mortalidad, mostrando un cuadro tanto clínico, como lesional característico de la infección experimental. Además, causaron la muerte al 100% de los animales entre el tercer y décimo día postinfección. En el estudio anatomopatológico la cepa del serogrupo Ballum y la del serogrupo Pomona produjeron focos de hemorragias específicamente en el riñón y pulmones. De forma similar ocurrió una congestión hepática y renal, mientras que la hemorragia renal fue observada con mayor frecuencia en la cepa del serogrupo Pomona, diferenciándose del resto de las cepas que mostraron esta lesión con menos frecuencia. Conclusiones. Este trabajo permitió una mayor caracterización de estas cepas siendo utilizadas como futuras candidatas vacunales frente a una nueva epidemia de Leptospirosis en Nicaragua. Rev.MVZ Córdoba 18(2):3484-3491, 2013. ORIGINAL 3485 ABSTRACTObjective. The aim of this study was to characterize the symptomatology and anatomopathological lesions caused by 5 clinical isolated Leptospira spp. from Nicaragua in a Mesocricetus auratus biomodel. Materials and methods. 50 hamsters were inoculated via i.p with 1mL of the culture of each strain in exponential phase having a cellular concentration of 7.5 x 10 6 leptospira/mL, (10 animals per strain). Signs of the disease, mortality during 14 days, and macroscopic and microscopic anatomopathological lesions by haematoxylin-eosin and Warthyn Starryn stain technique were evaluated. Results. All the strains presented high mortality, showing clinical lesions of the experimental infection. Death to 100% of the animals was caused between the third and tenth day post-infection. In the anatomopathologic study, the strains of the Ballum and Pomona serogroup produced haemorrhaging specifically in the kidney and lungs. The animals manifested hepatic and renal congestion, while the renal haemorrhage was observed with more frequency in the strain of the Pomona serogroup, differing from the other strains, which presented this lesion less frequently. Conclusions. This work allowed a better characterization of these strains in order to use them as future vaccine candidates for future Leptospirosis epidemics in Nicaragua.

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Section: Discussionunclassified

“…Por tanto una evaluación más completa de la capacidad de candidatos vacunales para inducir una respuesta inmunoprotectora. Esto requiere un panel bien caracterizado de cepas virulentas que representen los serovares de importancia tanto desde el punto de vista clínico y epidemiológico (6,8,13,29,30).…”

Section: Discussionunclassified

Estudio anatomopatológico de aislados de Leptospira spp., provenientes de Nicaragua en Mesocricetus auratus como biomodelo

et al. 2013

Rev MVZ Córdoba

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“…This is while; the available vaccines neither perused immunological memory nor confer cross-protection [1][2][3][4][5]. Nowadays the available commercial animal vaccines currently used worldwide are killed, whole-cell vaccines [6]. Adjuvant free form of this vaccine may fail to prevent renal shedding in infected animals [7].…”

Section: Introductionmentioning

confidence: 99%

Development of Leptospira Killed Whole Culture Vaccine Using Different Adjuvants and Evaluation of Humoral Immune Response in Hamsters

Banihashemi¹,

Baradaran²,

Tebianian³

et al. 2013

J Vaccines Vaccin

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Leptospirosis is an acute infectious disease caused by Leptospira spp. that produces a broad range of health problems mainly in developing world. In several affected countries, a major attempt has been begun to find a potent and effective vaccine. This is while; the available vaccines neither perused immunological memory nor confer cross-protection. Four-week-old male hamsters were subcutaneously inoculated with four-leptospiral vaccine and four-control adjuvant through a triple-injection regiment at fortnightly intervals. Animals were bled through the saphenous vein weekly until the seventh week. We have demonstrated that adjuvant plays a fundamental role in the type and extent of the immune responses raised by leptospiral vaccination. We strongly recommend selection of alum in any formulation of leptospiral vaccine.

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“…To assess cross-protection, standardized strains of different species and serogroups of Leptospira are needed, and few are available. [19][20][21][22][23] This work attempts to fill this void by describing the pathogenesis and LD50 of a Leptospira isolate, which is not only highly virulent but also belongs to a serogroup of growing importance in recent years. [10][11][12][13] Experimental reproduction of leptospirosis was successfully achieved in the hamster model using strain 4E.…”

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confidence: 99%

Highly Virulent Leptospira borgpetersenii Strain Characterized in the Hamster Model

Diniz

Félix²,

Bonel-Raposo³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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A recent study by our group reported the isolation and partial serological and molecular characterization of four Leptospira borgpetersenii serogroup Ballum strains. Here, we reproduced experimental leptospirosis in golden Syrian hamsters (Mesocricetus auratus) and carried out standardization of lethal dose 50% (LD50) of one of these strains (4E). Clinical disease features and histopathologic analyses of tissue lesions were also observed. As results, strain 4E induced lethality in the hamster model with inocula lower than 10 leptospires, and histopathological examination of animals showed typical lesions found in severe leptospirosis. Gross pathological findings were peculiar; animals that died early had more chance of presenting severe jaundice and less chance of presenting pulmonary hemorrhages (P < 0.01). L. borgpetersenii serogroup Ballum has had a considerable growth in human leptospirosis cases in recent years. This strain has now been thoroughly characterized and can be used in more studies, especially evaluations of vaccine candidates.

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Study on the efficacy of Leptospira vaccines developed from serovars isolated from Trinidad and comparison with commercial vaccines using a hamster model

Cited by 21 publications

References 32 publications

Estudio anatomopatológico de aislados de Leptospira spp., provenientes de Nicaragua en Mesocricetus auratus como biomodelo

Estudio anatomopatológico de aislados de Leptospira spp., provenientes de Nicaragua en Mesocricetus auratus como biomodelo

Development of Leptospira Killed Whole Culture Vaccine Using Different Adjuvants and Evaluation of Humoral Immune Response in Hamsters

Highly Virulent Leptospira borgpetersenii Strain Characterized in the Hamster Model

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