Study on the haplotypes of MICA and MICB microsatellite and HLA‐B locus in the Guangzhou Han population

Abstract

The purpose of this study was to investigate the genetic polymorphisms and haplotypes of microsatellite locus in exon 5 of the MICA gene and intron 1 of the MICB gene and human leukocyte antigen-B (HLA-B) gene based on 106 samples of the Guangzhou Han population through means of polymerase chain reaction and the fluorescent technique (6-FAM). The corresponding haplotype frequencies, linkage disequilibrium values and relative linkage disequilibrium values were estimated based on population data. The results show that the genotype distributions of MICA and MICB microsatellite and HLA-B satisfy the Hardy-Weinberg equilibrium. In total, five alleles of MICA microsatellite locus and 14 alleles of MICB microsatellite locus were observed. MICA A5 was the most common allele (0.2877), whereas A4 was the least common (0.1321). MICB CA14 was the most common allele (0.3255), and CA19 and CA28 were the two least common (0.0047). CA27 was not observed at all. Five kinds of MICA-MICB haplotypes, 18 kinds of MICA-HLA-B haplotypes and 12 kinds of MICB-HLA-B haplotypes occurred at frequencies of more than 1%. The common haplotypes of MICA-MICB, MICA-HLA-B and MICB-HLA-B were A5-CA14, A5.1-CA18, A4-CA26, A9-CA15, A5-B*15(62), A5.1-B*1301/1302, A4-B*1301/1302, A6-B*51, A6-B*4403, A9-B*3802, CA14-B*4601, CA18-B*1301/1302 and CA26-B*1301/1302, and these haplotypes showed strong linkage disequilibrium. The polymorphisms and haplotype distributions of MICA and MICB microsatellite and HLA-B locus in the Guangzhou Han population have their own distinct genetic characteristics. The microsatellite locus of exon 5 of the MICA gene and intron 1 of the MICB gene could therefore be used as genetic markers in the studies of anthropology, gene linkage analysis in genetic diseases, individual identification and paternity testing in forensic medicine.

HLA-B*1301 as a Biomarker for Genetic Susceptibility to Hypersensitivity Dermatitis Induced by Trichloroethylene among Workers in China

“…HLA-B*13 is one of a common allelic group (phenotype frequency ranges from 10 to 30%) found in the Chinese population, and the distribution of HLA-B alleles in the controls of the present study was similar to that of the general population in southern China (Feng et al 2004; Liang et al 2005; Shi et al 2006; Yang et al 2006). In addition, the HLA-B*1301 allele, a member of the HLA- B*13 group in the southern Chinese population, is a specific allele in Asians, but is absent in whites and blacks (Cao et al 2001; Lin et al 1995; Williams et al 2001).…”

“…The frequencies of HLA-B*1301 and HLA-B*1302 are variably distributed in different regions of China. The frequency of HLA-B*1301 in southern Chinese is higher than that in northern Chinese (Feng et al 2004; Middleton et al 2004; Shi et al 2006; Yang et al 2006). Rapid economic development, as occurring in China, could generate a highly mobile work force.…”

MHC microsatellites in a Southern Brazilian population

“…The strong association between STR_MICA and D6S2810 with the HLA‐B gene, described in previous population studies (Bolognesi et al. , 2001; Feng et al. , 2004; Grubic et al.…”

“…Nevertheless, the haplotypes STR_MICA*A4–HLA‐B*27 ; STR_MICA*A5–HLA‐B*35 ; STR_MICA*A5.1–HLA‐B*44, reported in these studies, were not observed or were not in significant LD in the present analysis. When our results were compared with those obtained from a Chinese population (Feng et al. , 2004; Tian et al.…”

Distribution of MICA haplotypes in a Chinese Han population

“…Similarly, discrepancies were detected in the distribution of MICA-STR (Table 5) [25]. However, these discrepancies were most likely the result of different techniques used in each study.…”