Study on the inhibition of hyperthermic CO2 pneumoperitoneum on the proliferation and migration of colon cancer cells and its mechanism

Abstract

Abstract. The present study explored the inhibitory effect of hyperthermic CO 2 pneumoperitoneum on the proliferation and migration of colon cancer cells, and its mechanism. Colon cancer cell line SW-480 was sealed into a urine collection bag to simulate pneumoperitoneum with 100% CO 2 under a pressure of 14 mmHg. The growth and morphology of cells were observed under a microscope, the inhibition on cell proliferation was measured using WST-8 test, cell apoptosis and the cell cycle were monitored using fluorescence-activated cell sorting analysis, the migration of cells was tested using the scratch assay, and the expression of HSP-70, caspase-3, hypoxiainducible factor-1α and matrix metalloproteinase-9 (MMP-9) proteins and genes was investigated using western blotting and reverse transcription polymerase chain reaction. Compared with the control group, there was no significant difference in the CO 2 group (P>0.05), while the apoptosis and necrosis rates in the hyperthermo-CO 2 group was significantly increased (P<0.05). Compared with the control group, the number of cells at G0/G1 phase significantly increased and the number of cells at S phase significantly decreased in the hyperthermo-CO 2 group (P<0.05), indicating that hyperthermo-CO 2 could arrest the cell cycle. It was suggested by the results of the scratch assay that cell migration ability enhanced in the CO 2 group, but decreased in the hyperthermo-CO 2 group compared with the control. CO 2 pneumoperitoneum promoted cell migration by upregulating HIF-1α and MMP-9 expression. However, the CO 2 pneumoperitoneum with hyperthermia enhanced apoptosis and inhibited migration by upregulating the expression of HSP-70, HIF-1α and caspase-3, but downregulating the expression of MMP-9.

Water‐Responsive Hybrid Nanoparticles Codelivering ICG and DOX Effectively Treat Breast Cancer via Hyperthermia‐aided DOX Functionality and Drug Penetration

“…However, the cell migration activities measured by the scratch and Transwell migration assay showed some interesting results. As shown in Figure S8B, C in the Supporting Information, results indicated that PL/ACC‐DOX&ICG plus irradiation could achieve distinct inhibitory effects on cell migration, likely owing to the inhibitory effects of mild hyperthermia on the expression of a variety of metastasis‐related factors . These results indicated that PTT combined with chemotherapy was capable of suppressing cell migration before inducing cell death.…”

Identification of Potential WSB1 Inhibitors by AlphaFold Modeling, Virtual Screening, and Molecular Dynamics Simulation Studies

“…HIF-l has been reported to regulate various related signaling pathways during the adaptation of tumor cells to the hypoxic environment and plays a crucial role in tumor cell proliferation, angiogenesis, malignant invasion, and metastasis [6][7][8][9]. For example, high expression of HIF-l has been associated with local in ltration and distal metastasis of tumors, including colon, prostate, and lung cancers [10,11].…”