Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Abstract

Traditional Chinese medicine (TCM), as a unique form of natural medicine, has been used in Chinese traditional therapeutic systems over two thousand years. Active components in Chinese herbal medicine are the material basis for the prevention and treatment of diseases. Research on drug-protein binding is one of the important contents in the study of early stage clinical pharmacokinetics of drugs. Plasma protein binding study has far-reaching influence on the pharmacokinetics and pharmacodynamics of drugs and helps to understand the basic rule of drug effects. It is important to study the binding characteristics of the active components in Chinese herbal medicine with plasma proteins for the medical science and modernization of TCM. This review summarizes the common analytical methods which are used to study the active herbal components-protein binding and gives the examples to illustrate their application. Rules and influence factors of the binding between different types of active herbal components and plasma proteins are summarized in the end. Finally, a suggestion on choosing the suitable technique for different types of active herbal components is provided, and the prospect of the drug-protein binding used in the area of TCM research is also discussed.

Study on the Interactions Between Caffeoylquinic Acids With Bovine Serum Albumin: Spectroscopy, Antioxidant Activity, LC-MSn, and Molecular Docking Approach

“…Therefore, the investigations of HSA binding with small molecules are of important significance. Bovine serum albumin (BSA) was the most common substitution model for HSA due to its highly similar tertiary structure (76.52%) (Gelamo and Tabak, 2000; Jiao et al, 2018).…”

“…Bin Tang et al thought that the binding capability of CGAs increased with the increased caffeoyl group and the substituted positions of caffeoyl group and the formation of ester bond in CQAs also could moderately effect the binding process (Tang et al, 2016). The binding of drug and protein is influenced by multiple factors, such as the pH, ionic concentration of solvents, temperatures, trace elements, and solvent types (Jiao et al, 2018). In generally, to accurately simulate the human body environment, PBS and Tris-HCl buffer are two most commonly used buffers in drug-protein binding experiment to keep the pH of the BSA solution at 7.40.…”

Molecular evidence of herbal formula: a network‐based analysis of Si‐Wu decoction

“…Among these compounds, the paeonol, gallic acid, paeonoside, etc. from BS, 24 scopoletin from DS, 25 and coniferyl ferulate from both DG and CX 26 are potential bioactive as reported. But, how these compounds work synergistically to regulate the balance of the human body still needs further exploration.…”

Synthesis and biological properties of a series of aryl alkyl disulfide derivatives

“…Fluorescence spectroscopy can provide information regarding the quenching mechanism, the binding constant and the number of binding sites involved in the binding interaction between plasma protein and a target molecule [19]. Fluorescence quenching can occur either by dynamic mechanism which involves diffusive encounters or by static mechanism which involves complex formation [20].…”